Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems

Abstract Mammalian genomes encode multiple layers of regulation, including a class of RNA molecules known as long non-coding RNAs (lncRNAs). These are >200 nucleotides in length and similar to mRNAs, they are capped, polyadenylated, and spliced. In contrast to mRNAs, lncRNAs are less abundant and...

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Autores principales:	Kathy Ushakov, Tal Koffler-Brill, Aviv Rom, Kobi Perl, Igor Ulitsky, Karen B. Avraham
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b034fbffe7de4cf5a380d2d43bb8a638
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spelling	oai:doaj.org-article:b034fbffe7de4cf5a380d2d43bb8a6382021-12-02T15:05:18ZGenome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems10.1038/s41598-017-08320-32045-2322https://doaj.org/article/b034fbffe7de4cf5a380d2d43bb8a6382017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08320-3https://doaj.org/toc/2045-2322Abstract Mammalian genomes encode multiple layers of regulation, including a class of RNA molecules known as long non-coding RNAs (lncRNAs). These are >200 nucleotides in length and similar to mRNAs, they are capped, polyadenylated, and spliced. In contrast to mRNAs, lncRNAs are less abundant and have higher tissue specificity, and have been linked to development, epigenetic processes, and disease. However, little is known about lncRNA function in the auditory and vestibular systems, or how they play a role in deafness and vestibular dysfunction. To help address this need, we performed a whole-genome identification of lncRNAs using RNA-seq at two developmental stages of the mouse inner ear sensory epithelium of the cochlea and vestibule. We identified 3,239 lncRNA genes, most of which were intergenic (lincRNAs) and 721 are novel. We examined temporal and tissue specificity by analyzing the developmental profiles on embryonic day 16.5 and at birth. The spatial and temporal patterns of three lncRNAs, two of which are in proximity to genes associated with hearing and deafness, were explored further. Our findings indicate that lncRNAs are prevalent in the sensory epithelium of the mouse inner ear and are likely to play key roles in regulating critical pathways for hearing and balance.Kathy UshakovTal Koffler-BrillAviv RomKobi PerlIgor UlitskyKaren B. AvrahamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kathy Ushakov Tal Koffler-Brill Aviv Rom Kobi Perl Igor Ulitsky Karen B. Avraham Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems
description	Abstract Mammalian genomes encode multiple layers of regulation, including a class of RNA molecules known as long non-coding RNAs (lncRNAs). These are >200 nucleotides in length and similar to mRNAs, they are capped, polyadenylated, and spliced. In contrast to mRNAs, lncRNAs are less abundant and have higher tissue specificity, and have been linked to development, epigenetic processes, and disease. However, little is known about lncRNA function in the auditory and vestibular systems, or how they play a role in deafness and vestibular dysfunction. To help address this need, we performed a whole-genome identification of lncRNAs using RNA-seq at two developmental stages of the mouse inner ear sensory epithelium of the cochlea and vestibule. We identified 3,239 lncRNA genes, most of which were intergenic (lincRNAs) and 721 are novel. We examined temporal and tissue specificity by analyzing the developmental profiles on embryonic day 16.5 and at birth. The spatial and temporal patterns of three lncRNAs, two of which are in proximity to genes associated with hearing and deafness, were explored further. Our findings indicate that lncRNAs are prevalent in the sensory epithelium of the mouse inner ear and are likely to play key roles in regulating critical pathways for hearing and balance.
format	article
author	Kathy Ushakov Tal Koffler-Brill Aviv Rom Kobi Perl Igor Ulitsky Karen B. Avraham
author_facet	Kathy Ushakov Tal Koffler-Brill Aviv Rom Kobi Perl Igor Ulitsky Karen B. Avraham
author_sort	Kathy Ushakov
title	Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems
title_short	Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems
title_full	Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems
title_fullStr	Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems
title_full_unstemmed	Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems
title_sort	genome-wide identification and expression profiling of long non-coding rnas in auditory and vestibular systems
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b034fbffe7de4cf5a380d2d43bb8a638
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Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems

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