Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish

Polybrominated diphenyl ethers are contaminants that are widely distributed in the environment and in food samples. In this study, a highly sensitive biotin–streptavidin system-based real-time immuno-polymerase chain reaction assay (BA-IPCR) was developed to detect 2,2′,4,4′-tetrabromodiphenyl ether...

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Autores principales:	Zhen Ma, Huisheng Zhuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	real-time immuno-polymerase chain reaction biotin–streptavidin system 2,2′,4,4′-tetrabromodiphenyl ether marine fish polyclonal antibody Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b038a4fb33d84af0b549e6d1afd66151
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spelling	oai:doaj.org-article:b038a4fb33d84af0b549e6d1afd661512021-11-26T11:19:46ZBiotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish0954-01051465-344310.1080/09540105.2018.1489374https://doaj.org/article/b038a4fb33d84af0b549e6d1afd661512018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1489374https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Polybrominated diphenyl ethers are contaminants that are widely distributed in the environment and in food samples. In this study, a highly sensitive biotin–streptavidin system-based real-time immuno-polymerase chain reaction assay (BA-IPCR) was developed to detect 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47). The BDE-47 immunogen and coating antigen were prepared, and a specific polyclonal anti-BDE-47 antibody was obtained. Several physiochemical factors in the immunoassay were optimised. The established BA-IPCR method was used to detect BDE-47 with a linearity ranging from 10 pg L−1 to 100 ng L−1. The limit of detection was 2.96 pg L−1. The recovery of the spiked samples was 92.97–107.19% and the coefficient of variation was 6.93–11.06%. The proposed method was applied to successfully detect BDE-47 in marine fish. The BA-IPCR data for the detection of BDE-47 were consistent with gas chromatography–mass spectrometry findings. The BA-IPCR method is accurate, sensitive and suitable for detecting trace amount of BDE-47 in food samples.Zhen MaHuisheng ZhuangTaylor & Francis Grouparticlereal-time immuno-polymerase chain reactionbiotin–streptavidin system2,2′,4,4′-tetrabromodiphenyl ethermarine fishpolyclonal antibodyAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1012-1027 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	real-time immuno-polymerase chain reaction biotin–streptavidin system 2,2′,4,4′-tetrabromodiphenyl ether marine fish polyclonal antibody Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	real-time immuno-polymerase chain reaction biotin–streptavidin system 2,2′,4,4′-tetrabromodiphenyl ether marine fish polyclonal antibody Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Zhen Ma Huisheng Zhuang Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish
description	Polybrominated diphenyl ethers are contaminants that are widely distributed in the environment and in food samples. In this study, a highly sensitive biotin–streptavidin system-based real-time immuno-polymerase chain reaction assay (BA-IPCR) was developed to detect 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47). The BDE-47 immunogen and coating antigen were prepared, and a specific polyclonal anti-BDE-47 antibody was obtained. Several physiochemical factors in the immunoassay were optimised. The established BA-IPCR method was used to detect BDE-47 with a linearity ranging from 10 pg L−1 to 100 ng L−1. The limit of detection was 2.96 pg L−1. The recovery of the spiked samples was 92.97–107.19% and the coefficient of variation was 6.93–11.06%. The proposed method was applied to successfully detect BDE-47 in marine fish. The BA-IPCR data for the detection of BDE-47 were consistent with gas chromatography–mass spectrometry findings. The BA-IPCR method is accurate, sensitive and suitable for detecting trace amount of BDE-47 in food samples.
format	article
author	Zhen Ma Huisheng Zhuang
author_facet	Zhen Ma Huisheng Zhuang
author_sort	Zhen Ma
title	Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish
title_short	Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish
title_full	Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish
title_fullStr	Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish
title_full_unstemmed	Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish
title_sort	biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/b038a4fb33d84af0b549e6d1afd66151
work_keys_str_mv	AT zhenma biotinstreptavidinsystembasedrealtimeimmunopolymerasechainreactionforsensitivedetectionof2244tetrabromodiphenyletherinmarinefish AT huishengzhuang biotinstreptavidinsystembasedrealtimeimmunopolymerasechainreactionforsensitivedetectionof2244tetrabromodiphenyletherinmarinefish
_version_	1718409555497451520

Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish

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