Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method

Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method

Xue-Feng Zhao1,2, Xiao-Dong Li3, Yun-Qing Kang4, Quan Yuan1,21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People's Republic of China; 2West China School of Stomatology, Sichuan University, Chengdu, People's Republic of China; 3Affiliated Hospital of St...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Zhao XF, Li XD, Kang YQ, Yuan Q
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2011
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/b03bc77830ee4f42b17d444d237cb97a
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:b03bc77830ee4f42b17d444d237cb97a
record_format dspace
spelling oai:doaj.org-article:b03bc77830ee4f42b17d444d237cb97a2021-12-02T01:07:58ZImproved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method1176-91141178-2013https://doaj.org/article/b03bc77830ee4f42b17d444d237cb97a2011-07-01T00:00:00Zhttp://www.dovepress.com/improved-biocompatibility-of-novel-polyl-lactic-acidszlig-tricalcium-p-a7794https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xue-Feng Zhao1,2, Xiao-Dong Li3, Yun-Qing Kang4, Quan Yuan1,21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People's Republic of China; 2West China School of Stomatology, Sichuan University, Chengdu, People's Republic of China; 3Affiliated Hospital of Stomatology and College of Stomatology, Chongqing Medical University, Chongqing, People's Republic of China; 4College of Materials Science and Engineering, Sichuan University, Chengdu, People's Republic of ChinaAbstract: A porous poly(L-lactic acid)/ß-tricalcium phosphate (PLLA/ß-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously interconnected porous structure with a porosity of 70% and compressive strength of 1.35 MPa. The methylthiazol tetrazolium values and alkaline phosphatase (ALP) activity of osteoblasts seeded on the solvent-free scaffold were significant higher than those of the control. Using real-time PCR, gene expressions of ALP, osteocalcin, and type 1 collagen were shown to be upregulated. As the method does not use any organic solvent, it eliminates problems associated with organic solvent residue and therefore improves the cell compatibility. It has a promising potential for the preparation of porous scaffold for bone tissue engineering.Keywords: biocompatibility, biomaterials, composites, poly(L-lactic acid), ß-tricalcium phosphateZhao XFLi XDKang YQYuan QDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1385-1390 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Zhao XF
Li XD
Kang YQ
Yuan Q
Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method
description Xue-Feng Zhao1,2, Xiao-Dong Li3, Yun-Qing Kang4, Quan Yuan1,21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People's Republic of China; 2West China School of Stomatology, Sichuan University, Chengdu, People's Republic of China; 3Affiliated Hospital of Stomatology and College of Stomatology, Chongqing Medical University, Chongqing, People's Republic of China; 4College of Materials Science and Engineering, Sichuan University, Chengdu, People's Republic of ChinaAbstract: A porous poly(L-lactic acid)/ß-tricalcium phosphate (PLLA/ß-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously interconnected porous structure with a porosity of 70% and compressive strength of 1.35 MPa. The methylthiazol tetrazolium values and alkaline phosphatase (ALP) activity of osteoblasts seeded on the solvent-free scaffold were significant higher than those of the control. Using real-time PCR, gene expressions of ALP, osteocalcin, and type 1 collagen were shown to be upregulated. As the method does not use any organic solvent, it eliminates problems associated with organic solvent residue and therefore improves the cell compatibility. It has a promising potential for the preparation of porous scaffold for bone tissue engineering.Keywords: biocompatibility, biomaterials, composites, poly(L-lactic acid), ß-tricalcium phosphate
format article
author Zhao XF
Li XD
Kang YQ
Yuan Q
author_facet Zhao XF
Li XD
Kang YQ
Yuan Q
author_sort Zhao XF
title Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method
title_short Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method
title_full Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method
title_fullStr Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method
title_full_unstemmed Improved biocompatibility of novel poly(L-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method
title_sort improved biocompatibility of novel poly(l-lactic acid)/ß-tricalcium phosphate scaffolds prepared by an organic solvent-free method
publisher Dove Medical Press
publishDate 2011
url https://doaj.org/article/b03bc77830ee4f42b17d444d237cb97a
work_keys_str_mv AT zhaoxf improvedbiocompatibilityofnovelpolyllacticacidampszligtricalciumphosphatescaffoldspreparedbyanorganicsolventfreemethod
AT lixd improvedbiocompatibilityofnovelpolyllacticacidampszligtricalciumphosphatescaffoldspreparedbyanorganicsolventfreemethod
AT kangyq improvedbiocompatibilityofnovelpolyllacticacidampszligtricalciumphosphatescaffoldspreparedbyanorganicsolventfreemethod
AT yuanq improvedbiocompatibilityofnovelpolyllacticacidampszligtricalciumphosphatescaffoldspreparedbyanorganicsolventfreemethod
_version_ 1718403278872510464

Ejemplares similares