Ultrasensitive immunochromatographic strip for detection of cyproheptadine

Using immunological and cell fusion technology, group-specific monoclonal antibodies were produced against cyproheptadine. The 50% inhibitory concentration of the antibody for cyproheptadine was 0.37 ng/mL, the linear range was 0.16–0.85 ng/mL, and recoveries of antibody in spiked negative pig urine...

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Autores principales:	Mengyuan Guo, Li Sun, Liqiang Liu, Shanshan Song, Hua Kuang, Gang Cui
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	cyproheptadine indirect competitive enzyme-linked immunosorbent assay (ic-elisa) immunochromatographic test strip urine Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b03f7302b7ad4d049525ce3e778582bf
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spelling	oai:doaj.org-article:b03f7302b7ad4d049525ce3e778582bf2021-11-26T11:19:46ZUltrasensitive immunochromatographic strip for detection of cyproheptadine0954-01051465-344310.1080/09540105.2018.1490395https://doaj.org/article/b03f7302b7ad4d049525ce3e778582bf2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1490395https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Using immunological and cell fusion technology, group-specific monoclonal antibodies were produced against cyproheptadine. The 50% inhibitory concentration of the antibody for cyproheptadine was 0.37 ng/mL, the linear range was 0.16–0.85 ng/mL, and recoveries of antibody in spiked negative pig urine were 80–120%. An ultrasensitive immunochromatographic strip, based on the antibody, was developed for the rapid detection of cyproheptadine in pig urine. The test strip had a cut-off value of 5 ng/mL.Mengyuan GuoLi SunLiqiang LiuShanshan SongHua KuangGang CuiTaylor & Francis Grouparticlecyproheptadineindirect competitive enzyme-linked immunosorbent assay (ic-elisa)immunochromatographic test stripurineAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 941-952 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyproheptadine indirect competitive enzyme-linked immunosorbent assay (ic-elisa) immunochromatographic test strip urine Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	cyproheptadine indirect competitive enzyme-linked immunosorbent assay (ic-elisa) immunochromatographic test strip urine Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Mengyuan Guo Li Sun Liqiang Liu Shanshan Song Hua Kuang Gang Cui Ultrasensitive immunochromatographic strip for detection of cyproheptadine
description	Using immunological and cell fusion technology, group-specific monoclonal antibodies were produced against cyproheptadine. The 50% inhibitory concentration of the antibody for cyproheptadine was 0.37 ng/mL, the linear range was 0.16–0.85 ng/mL, and recoveries of antibody in spiked negative pig urine were 80–120%. An ultrasensitive immunochromatographic strip, based on the antibody, was developed for the rapid detection of cyproheptadine in pig urine. The test strip had a cut-off value of 5 ng/mL.
format	article
author	Mengyuan Guo Li Sun Liqiang Liu Shanshan Song Hua Kuang Gang Cui
author_facet	Mengyuan Guo Li Sun Liqiang Liu Shanshan Song Hua Kuang Gang Cui
author_sort	Mengyuan Guo
title	Ultrasensitive immunochromatographic strip for detection of cyproheptadine
title_short	Ultrasensitive immunochromatographic strip for detection of cyproheptadine
title_full	Ultrasensitive immunochromatographic strip for detection of cyproheptadine
title_fullStr	Ultrasensitive immunochromatographic strip for detection of cyproheptadine
title_full_unstemmed	Ultrasensitive immunochromatographic strip for detection of cyproheptadine
title_sort	ultrasensitive immunochromatographic strip for detection of cyproheptadine
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/b03f7302b7ad4d049525ce3e778582bf
work_keys_str_mv	AT mengyuanguo ultrasensitiveimmunochromatographicstripfordetectionofcyproheptadine AT lisun ultrasensitiveimmunochromatographicstripfordetectionofcyproheptadine AT liqiangliu ultrasensitiveimmunochromatographicstripfordetectionofcyproheptadine AT shanshansong ultrasensitiveimmunochromatographicstripfordetectionofcyproheptadine AT huakuang ultrasensitiveimmunochromatographicstripfordetectionofcyproheptadine AT gangcui ultrasensitiveimmunochromatographicstripfordetectionofcyproheptadine
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Ultrasensitive immunochromatographic strip for detection of cyproheptadine

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