Application of cryopreservation to tooth germ transplantation for root development and tooth eruption

Abstract We cryopreserved mouse tooth germs with widely open cervical margins of the enamel organ to overcome difficulties in cryoprotectant permeation and tested their efficacy by transplanting them into recipient mice. The upper right first molar germs of 8-day-old donor mice were extracted and ca...

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Autores principales:	Xinghan Li, Megumi Nakamura, Weidong Tian, Yasuyuki Sasano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b045c480fca34149b7f6f837efa8e99c
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spelling	oai:doaj.org-article:b045c480fca34149b7f6f837efa8e99c2021-12-02T14:49:26ZApplication of cryopreservation to tooth germ transplantation for root development and tooth eruption10.1038/s41598-021-88975-12045-2322https://doaj.org/article/b045c480fca34149b7f6f837efa8e99c2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88975-1https://doaj.org/toc/2045-2322Abstract We cryopreserved mouse tooth germs with widely open cervical margins of the enamel organ to overcome difficulties in cryoprotectant permeation and tested their efficacy by transplanting them into recipient mice. The upper right first molar germs of 8-day-old donor mice were extracted and categorized into the following four groups according to cryopreservation time: no cryopreservation, 1 week, 1 month, and 3 months. The donor tooth germs were transplanted into the upper right first molar germ sockets of the 8-day-old recipient mice. The upper left first molars of the recipient mice were used as controls. The outcome of the transplantation was assessed at 1, 2, and 3 weeks after transplantation. Stereomicroscopic evaluation revealed that most of the transplanted teeth erupted by 3 weeks after transplantation. Micro-computed tomography analysis revealed root elongation in the transplanted groups as well as in the controls. There was no significant difference between the cryopreserved and non-cryopreserved transplanted teeth, but the roots of the cryopreserved teeth were significantly shorter than those of the control teeth. Histological examination revealed root and periodontal ligament formations in all the transplanted groups. These results suggest that the transplantation of cryopreserved tooth germs facilitates subsequent root elongation and tooth eruption.Xinghan LiMegumi NakamuraWeidong TianYasuyuki SasanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xinghan Li Megumi Nakamura Weidong Tian Yasuyuki Sasano Application of cryopreservation to tooth germ transplantation for root development and tooth eruption
description	Abstract We cryopreserved mouse tooth germs with widely open cervical margins of the enamel organ to overcome difficulties in cryoprotectant permeation and tested their efficacy by transplanting them into recipient mice. The upper right first molar germs of 8-day-old donor mice were extracted and categorized into the following four groups according to cryopreservation time: no cryopreservation, 1 week, 1 month, and 3 months. The donor tooth germs were transplanted into the upper right first molar germ sockets of the 8-day-old recipient mice. The upper left first molars of the recipient mice were used as controls. The outcome of the transplantation was assessed at 1, 2, and 3 weeks after transplantation. Stereomicroscopic evaluation revealed that most of the transplanted teeth erupted by 3 weeks after transplantation. Micro-computed tomography analysis revealed root elongation in the transplanted groups as well as in the controls. There was no significant difference between the cryopreserved and non-cryopreserved transplanted teeth, but the roots of the cryopreserved teeth were significantly shorter than those of the control teeth. Histological examination revealed root and periodontal ligament formations in all the transplanted groups. These results suggest that the transplantation of cryopreserved tooth germs facilitates subsequent root elongation and tooth eruption.
format	article
author	Xinghan Li Megumi Nakamura Weidong Tian Yasuyuki Sasano
author_facet	Xinghan Li Megumi Nakamura Weidong Tian Yasuyuki Sasano
author_sort	Xinghan Li
title	Application of cryopreservation to tooth germ transplantation for root development and tooth eruption
title_short	Application of cryopreservation to tooth germ transplantation for root development and tooth eruption
title_full	Application of cryopreservation to tooth germ transplantation for root development and tooth eruption
title_fullStr	Application of cryopreservation to tooth germ transplantation for root development and tooth eruption
title_full_unstemmed	Application of cryopreservation to tooth germ transplantation for root development and tooth eruption
title_sort	application of cryopreservation to tooth germ transplantation for root development and tooth eruption
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b045c480fca34149b7f6f837efa8e99c
work_keys_str_mv	AT xinghanli applicationofcryopreservationtotoothgermtransplantationforrootdevelopmentandtootheruption AT meguminakamura applicationofcryopreservationtotoothgermtransplantationforrootdevelopmentandtootheruption AT weidongtian applicationofcryopreservationtotoothgermtransplantationforrootdevelopmentandtootheruption AT yasuyukisasano applicationofcryopreservationtotoothgermtransplantationforrootdevelopmentandtootheruption
_version_	1718389467513880576

Application of cryopreservation to tooth germ transplantation for root development and tooth eruption

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