Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr

Abstract Tilia species are valuable woody species due to their beautiful shape and role as honey trees. Somatic embryogenesis can be an alternative method for mass propagation of T. amurensis. However, the molecular mechanisms of T. amurensis somatic embryogenesis are yet to be known. Here, we condu...

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Autores principales:	Hye-In Kang, Chae-Bin Lee, Soon-Ho Kwon, Ji-Min Park, Kyu-Suk Kang, Donghwan Shim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b05679bc9f2a48a7bb7a33bb65c6fc0e
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spelling	oai:doaj.org-article:b05679bc9f2a48a7bb7a33bb65c6fc0e2021-12-02T11:39:27ZComparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr10.1038/s41598-021-85886-z2045-2322https://doaj.org/article/b05679bc9f2a48a7bb7a33bb65c6fc0e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85886-zhttps://doaj.org/toc/2045-2322Abstract Tilia species are valuable woody species due to their beautiful shape and role as honey trees. Somatic embryogenesis can be an alternative method for mass propagation of T. amurensis. However, the molecular mechanisms of T. amurensis somatic embryogenesis are yet to be known. Here, we conducted comparative transcriptional analysis during somatic embryogenesis of T. amurensis. RNA-Seq identified 1505 differentially expressed genes, including developmental regulatory genes. Auxin related genes such as YUC, AUX/IAA and ARF and signal transduction pathway related genes including LEA and SERK were differentially regulated during somatic embryogenesis. Also, B3 domain family (LEC2, FUS3), VAL and PKL, the regulatory transcription factors, were differentially expressed by somatic embryo developmental stages. Our results could provide plausible pathway of signaling somatic embryogenesis of T. amurensis, and serve an important resource for further studies in direct somatic embryogenesis in woody plants.Hye-In KangChae-Bin LeeSoon-Ho KwonJi-Min ParkKyu-Suk KangDonghwan ShimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hye-In Kang Chae-Bin Lee Soon-Ho Kwon Ji-Min Park Kyu-Suk Kang Donghwan Shim Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr
description	Abstract Tilia species are valuable woody species due to their beautiful shape and role as honey trees. Somatic embryogenesis can be an alternative method for mass propagation of T. amurensis. However, the molecular mechanisms of T. amurensis somatic embryogenesis are yet to be known. Here, we conducted comparative transcriptional analysis during somatic embryogenesis of T. amurensis. RNA-Seq identified 1505 differentially expressed genes, including developmental regulatory genes. Auxin related genes such as YUC, AUX/IAA and ARF and signal transduction pathway related genes including LEA and SERK were differentially regulated during somatic embryogenesis. Also, B3 domain family (LEC2, FUS3), VAL and PKL, the regulatory transcription factors, were differentially expressed by somatic embryo developmental stages. Our results could provide plausible pathway of signaling somatic embryogenesis of T. amurensis, and serve an important resource for further studies in direct somatic embryogenesis in woody plants.
format	article
author	Hye-In Kang Chae-Bin Lee Soon-Ho Kwon Ji-Min Park Kyu-Suk Kang Donghwan Shim
author_facet	Hye-In Kang Chae-Bin Lee Soon-Ho Kwon Ji-Min Park Kyu-Suk Kang Donghwan Shim
author_sort	Hye-In Kang
title	Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr
title_short	Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr
title_full	Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr
title_fullStr	Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr
title_full_unstemmed	Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr
title_sort	comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in tilia amurensis rupr
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b05679bc9f2a48a7bb7a33bb65c6fc0e
work_keys_str_mv	AT hyeinkang comparativetranscriptomeanalysisduringdevelopmentalstagesofdirectsomaticembryogenesisintiliaamurensisrupr AT chaebinlee comparativetranscriptomeanalysisduringdevelopmentalstagesofdirectsomaticembryogenesisintiliaamurensisrupr AT soonhokwon comparativetranscriptomeanalysisduringdevelopmentalstagesofdirectsomaticembryogenesisintiliaamurensisrupr AT jiminpark comparativetranscriptomeanalysisduringdevelopmentalstagesofdirectsomaticembryogenesisintiliaamurensisrupr AT kyusukkang comparativetranscriptomeanalysisduringdevelopmentalstagesofdirectsomaticembryogenesisintiliaamurensisrupr AT donghwanshim comparativetranscriptomeanalysisduringdevelopmentalstagesofdirectsomaticembryogenesisintiliaamurensisrupr
_version_	1718395720260648960

Comparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr

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