Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease

Celiac disease (CD) is a chronic intestinal inflammation caused by gluten ingestion in genetically predisposed individuals. Overt-CD and potential-CD are the two main forms of gluten intolerance in pediatric patients with different grades of intestinal mucosa lesion and clinical management. For over...

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Autores principales:	Serena Vitale, Mariantonia Maglio, Stefania Picascia, Ilaria Mottola, Erasmo Miele, Riccardo Troncone, Renata Auricchio, Carmen Gianfrani
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pediatric celiac disease biomarkers villous atrophy TCRγδ+ T cells IL4 translational research Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/b05c17d4b88e4b8c9e65597280e87dbb
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spelling	oai:doaj.org-article:b05c17d4b88e4b8c9e65597280e87dbb2021-11-25T18:42:33ZIntestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease10.3390/pharmaceutics131119711999-4923https://doaj.org/article/b05c17d4b88e4b8c9e65597280e87dbb2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1971https://doaj.org/toc/1999-4923Celiac disease (CD) is a chronic intestinal inflammation caused by gluten ingestion in genetically predisposed individuals. Overt-CD and potential-CD are the two main forms of gluten intolerance in pediatric patients with different grades of intestinal mucosa lesion and clinical management. For overt-CD patients the gluten-free diet is mandatory, while for potential-CD the dietary therapy is recommended only for those subjects becoming clinically symptomatic overtime. To date, specific early biomarkers of evolution to villous atrophy in potential-CD are lacking. We recently observed an expansion of TCRγδ+ T cells and a concomitant disappearance of IL4-producing T cells in the intestinal mucosa of overt-CD patients compared to potential-CD children, suggesting the involvement of these two cells subsets in the transition from potential-CD to overt-CD. In this study, we demonstrated that the intestinal densities of IL4+ T cells inversely correlated with TCRγδ+ T cell expansion (<i>p</i> < 0.005) and with the serum levels of anti-tissue transglutaminase antibodies (<i>p</i> < 0.01). The changes of these two cell subsets strongly correlated with mucosal lesions, according to the histological Marsh classification, as the transition from M0 to M3 lesions was associated with a significant reduction of IL4+ T cells (M0 vs. M1 <i>p</i> < 0.04, M0 vs. M3 <i>p</i> < 0.007) and an increase of TCRγδ+ T cells (M0 vs. M1 <i>p</i> < 0.05, M0 vs. M3 <i>p</i> < 0.0006). These findings strongly suggest that the detection of TCRγδ+ and IL4+ T cells could serve as cellular biomarkers of mucosal lesion and targets of novel immunomodulatory therapies for CD.Serena VitaleMariantonia MaglioStefania PicasciaIlaria MottolaErasmo MieleRiccardo TronconeRenata AuricchioCarmen GianfraniMDPI AGarticlepediatric celiac diseasebiomarkersvillous atrophyTCRγδ+ T cellsIL4translational researchPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1971, p 1971 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pediatric celiac disease biomarkers villous atrophy TCRγδ+ T cells IL4 translational research Pharmacy and materia medica RS1-441
spellingShingle	pediatric celiac disease biomarkers villous atrophy TCRγδ+ T cells IL4 translational research Pharmacy and materia medica RS1-441 Serena Vitale Mariantonia Maglio Stefania Picascia Ilaria Mottola Erasmo Miele Riccardo Troncone Renata Auricchio Carmen Gianfrani Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
description	Celiac disease (CD) is a chronic intestinal inflammation caused by gluten ingestion in genetically predisposed individuals. Overt-CD and potential-CD are the two main forms of gluten intolerance in pediatric patients with different grades of intestinal mucosa lesion and clinical management. For overt-CD patients the gluten-free diet is mandatory, while for potential-CD the dietary therapy is recommended only for those subjects becoming clinically symptomatic overtime. To date, specific early biomarkers of evolution to villous atrophy in potential-CD are lacking. We recently observed an expansion of TCRγδ+ T cells and a concomitant disappearance of IL4-producing T cells in the intestinal mucosa of overt-CD patients compared to potential-CD children, suggesting the involvement of these two cells subsets in the transition from potential-CD to overt-CD. In this study, we demonstrated that the intestinal densities of IL4+ T cells inversely correlated with TCRγδ+ T cell expansion (<i>p</i> < 0.005) and with the serum levels of anti-tissue transglutaminase antibodies (<i>p</i> < 0.01). The changes of these two cell subsets strongly correlated with mucosal lesions, according to the histological Marsh classification, as the transition from M0 to M3 lesions was associated with a significant reduction of IL4+ T cells (M0 vs. M1 <i>p</i> < 0.04, M0 vs. M3 <i>p</i> < 0.007) and an increase of TCRγδ+ T cells (M0 vs. M1 <i>p</i> < 0.05, M0 vs. M3 <i>p</i> < 0.0006). These findings strongly suggest that the detection of TCRγδ+ and IL4+ T cells could serve as cellular biomarkers of mucosal lesion and targets of novel immunomodulatory therapies for CD.
format	article
author	Serena Vitale Mariantonia Maglio Stefania Picascia Ilaria Mottola Erasmo Miele Riccardo Troncone Renata Auricchio Carmen Gianfrani
author_facet	Serena Vitale Mariantonia Maglio Stefania Picascia Ilaria Mottola Erasmo Miele Riccardo Troncone Renata Auricchio Carmen Gianfrani
author_sort	Serena Vitale
title	Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_short	Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_full	Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_fullStr	Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_full_unstemmed	Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease
title_sort	intestinal cellular biomarkers of mucosal lesion progression in pediatric celiac disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b05c17d4b88e4b8c9e65597280e87dbb
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Intestinal Cellular Biomarkers of Mucosal Lesion Progression in Pediatric Celiac Disease

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