Genome evolution of a tertiary dinoflagellate plastid.

The dinoflagellates have repeatedly replaced their ancestral peridinin-plastid by plastids derived from a variety of algal lineages ranging from green algae to diatoms. Here, we have characterized the genome of a dinoflagellate plastid of tertiary origin in order to understand the evolutionary proce...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tove M Gabrielsen, Marianne A Minge, Mari Espelund, Ave Tooming-Klunderud, Vishwanath Patil, Alexander J Nederbragt, Christian Otis, Monique Turmel, Kamran Shalchian-Tabrizi, Claude Lemieux, Kjetill S Jakobsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b064666a127c4a6faa83081591837b18
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b064666a127c4a6faa83081591837b18
record_format	dspace
spelling	oai:doaj.org-article:b064666a127c4a6faa83081591837b182021-11-18T06:55:09ZGenome evolution of a tertiary dinoflagellate plastid.1932-620310.1371/journal.pone.0019132https://doaj.org/article/b064666a127c4a6faa83081591837b182011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21541332/?tool=EBIhttps://doaj.org/toc/1932-6203The dinoflagellates have repeatedly replaced their ancestral peridinin-plastid by plastids derived from a variety of algal lineages ranging from green algae to diatoms. Here, we have characterized the genome of a dinoflagellate plastid of tertiary origin in order to understand the evolutionary processes that have shaped the organelle since it was acquired as a symbiont cell. To address this, the genome of the haptophyte-derived plastid in Karlodinium veneficum was analyzed by Sanger sequencing of library clones and 454 pyrosequencing of plastid enriched DNA fractions. The sequences were assembled into a single contig of 143 kb, encoding 70 proteins, 3 rRNAs and a nearly full set of tRNAs. Comparative genomics revealed massive rearrangements and gene losses compared to the haptophyte plastid; only a small fraction of the gene clusters usually found in haptophytes as well as other types of plastids are present in K. veneficum. Despite the reduced number of genes, the K. veneficum plastid genome has retained a large size due to expanded intergenic regions. Some of the plastid genes are highly diverged and may be pseudogenes or subject to RNA editing. Gene losses and rearrangements are also features of the genomes of the peridinin-containing plastids, apicomplexa and Chromera, suggesting that the evolutionary processes that once shaped these plastids have occurred at multiple independent occasions over the history of the Alveolata.Tove M GabrielsenMarianne A MingeMari EspelundAve Tooming-KlunderudVishwanath PatilAlexander J NederbragtChristian OtisMonique TurmelKamran Shalchian-TabriziClaude LemieuxKjetill S JakobsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e19132 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tove M Gabrielsen Marianne A Minge Mari Espelund Ave Tooming-Klunderud Vishwanath Patil Alexander J Nederbragt Christian Otis Monique Turmel Kamran Shalchian-Tabrizi Claude Lemieux Kjetill S Jakobsen Genome evolution of a tertiary dinoflagellate plastid.
description	The dinoflagellates have repeatedly replaced their ancestral peridinin-plastid by plastids derived from a variety of algal lineages ranging from green algae to diatoms. Here, we have characterized the genome of a dinoflagellate plastid of tertiary origin in order to understand the evolutionary processes that have shaped the organelle since it was acquired as a symbiont cell. To address this, the genome of the haptophyte-derived plastid in Karlodinium veneficum was analyzed by Sanger sequencing of library clones and 454 pyrosequencing of plastid enriched DNA fractions. The sequences were assembled into a single contig of 143 kb, encoding 70 proteins, 3 rRNAs and a nearly full set of tRNAs. Comparative genomics revealed massive rearrangements and gene losses compared to the haptophyte plastid; only a small fraction of the gene clusters usually found in haptophytes as well as other types of plastids are present in K. veneficum. Despite the reduced number of genes, the K. veneficum plastid genome has retained a large size due to expanded intergenic regions. Some of the plastid genes are highly diverged and may be pseudogenes or subject to RNA editing. Gene losses and rearrangements are also features of the genomes of the peridinin-containing plastids, apicomplexa and Chromera, suggesting that the evolutionary processes that once shaped these plastids have occurred at multiple independent occasions over the history of the Alveolata.
format	article
author	Tove M Gabrielsen Marianne A Minge Mari Espelund Ave Tooming-Klunderud Vishwanath Patil Alexander J Nederbragt Christian Otis Monique Turmel Kamran Shalchian-Tabrizi Claude Lemieux Kjetill S Jakobsen
author_facet	Tove M Gabrielsen Marianne A Minge Mari Espelund Ave Tooming-Klunderud Vishwanath Patil Alexander J Nederbragt Christian Otis Monique Turmel Kamran Shalchian-Tabrizi Claude Lemieux Kjetill S Jakobsen
author_sort	Tove M Gabrielsen
title	Genome evolution of a tertiary dinoflagellate plastid.
title_short	Genome evolution of a tertiary dinoflagellate plastid.
title_full	Genome evolution of a tertiary dinoflagellate plastid.
title_fullStr	Genome evolution of a tertiary dinoflagellate plastid.
title_full_unstemmed	Genome evolution of a tertiary dinoflagellate plastid.
title_sort	genome evolution of a tertiary dinoflagellate plastid.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/b064666a127c4a6faa83081591837b18
work_keys_str_mv	AT tovemgabrielsen genomeevolutionofatertiarydinoflagellateplastid AT marianneaminge genomeevolutionofatertiarydinoflagellateplastid AT mariespelund genomeevolutionofatertiarydinoflagellateplastid AT avetoomingklunderud genomeevolutionofatertiarydinoflagellateplastid AT vishwanathpatil genomeevolutionofatertiarydinoflagellateplastid AT alexanderjnederbragt genomeevolutionofatertiarydinoflagellateplastid AT christianotis genomeevolutionofatertiarydinoflagellateplastid AT moniqueturmel genomeevolutionofatertiarydinoflagellateplastid AT kamranshalchiantabrizi genomeevolutionofatertiarydinoflagellateplastid AT claudelemieux genomeevolutionofatertiarydinoflagellateplastid AT kjetillsjakobsen genomeevolutionofatertiarydinoflagellateplastid
_version_	1718424249594544128

Genome evolution of a tertiary dinoflagellate plastid.

Ejemplares similares