The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

Glioma is the most common primary intracranial malignant tumour in adults. It has a high incidence and poses a serious threat to human health. Circular RNA is a hotspot of cancer research. In this study, we aimed to explore the role of circ_0001367 in gliomagenesis and the underlying mechanism. Firs...

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Autores principales:	Xuchen Dong, Peng Zhang, Liang Liu, Haoran Li, Shan Cheng, Suwen Li, Yuan Wang, Chaonan Zheng, Jun Dong, Li Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	circRNA hsa_circ_0001367 miR-545-3p LUZP1 glioma Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/b06d6ac110d5489fb4b795d628490167
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spelling	oai:doaj.org-article:b06d6ac110d5489fb4b795d6284901672021-11-18T08:58:14ZThe Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells2234-943X10.3389/fonc.2021.781471https://doaj.org/article/b06d6ac110d5489fb4b795d6284901672021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.781471/fullhttps://doaj.org/toc/2234-943XGlioma is the most common primary intracranial malignant tumour in adults. It has a high incidence and poses a serious threat to human health. Circular RNA is a hotspot of cancer research. In this study, we aimed to explore the role of circ_0001367 in gliomagenesis and the underlying mechanism. First, qRT-PCR was conducted, which showed that circ_0001367 level was downregulated in glioma tissues and cells. Next, gain-of-function and loss-of-function assays were performed, which indicated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells. Subsequent bioinformatics analysis, dual-luciferase reporter assays, RNA immunoprecipitation assays and cell function assays demonstrated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells by absorbing miR-545-3p and thereby regulating the expression of leucine zipper protein (LUZP1). Finally, an in vivo experiment was conducted, which demonstrated that circ_0001367 inhibited glioma growth in vivo by modulating miR-545-3p and LUZP1. Taken together, the results of this study demonstrate that the circ_0001367/miR-545-3p/LUZP1 axis may be a novel target for glioma therapy.Xuchen DongXuchen DongPeng ZhangPeng ZhangLiang LiuHaoran LiShan ChengSuwen LiYuan WangChaonan ZhengJun DongLi ZhangFrontiers Media S.A.articlecircRNAhsa_circ_0001367miR-545-3pLUZP1gliomaNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circRNA hsa_circ_0001367 miR-545-3p LUZP1 glioma Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	circRNA hsa_circ_0001367 miR-545-3p LUZP1 glioma Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Xuchen Dong Xuchen Dong Peng Zhang Peng Zhang Liang Liu Haoran Li Shan Cheng Suwen Li Yuan Wang Chaonan Zheng Jun Dong Li Zhang The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells
description	Glioma is the most common primary intracranial malignant tumour in adults. It has a high incidence and poses a serious threat to human health. Circular RNA is a hotspot of cancer research. In this study, we aimed to explore the role of circ_0001367 in gliomagenesis and the underlying mechanism. First, qRT-PCR was conducted, which showed that circ_0001367 level was downregulated in glioma tissues and cells. Next, gain-of-function and loss-of-function assays were performed, which indicated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells. Subsequent bioinformatics analysis, dual-luciferase reporter assays, RNA immunoprecipitation assays and cell function assays demonstrated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells by absorbing miR-545-3p and thereby regulating the expression of leucine zipper protein (LUZP1). Finally, an in vivo experiment was conducted, which demonstrated that circ_0001367 inhibited glioma growth in vivo by modulating miR-545-3p and LUZP1. Taken together, the results of this study demonstrate that the circ_0001367/miR-545-3p/LUZP1 axis may be a novel target for glioma therapy.
format	article
author	Xuchen Dong Xuchen Dong Peng Zhang Peng Zhang Liang Liu Haoran Li Shan Cheng Suwen Li Yuan Wang Chaonan Zheng Jun Dong Li Zhang
author_facet	Xuchen Dong Xuchen Dong Peng Zhang Peng Zhang Liang Liu Haoran Li Shan Cheng Suwen Li Yuan Wang Chaonan Zheng Jun Dong Li Zhang
author_sort	Xuchen Dong
title	The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells
title_short	The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells
title_full	The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells
title_fullStr	The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells
title_full_unstemmed	The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells
title_sort	circ_0001367/mir-545-3p/luzp1 axis regulates cell proliferation, migration and invasion in glioma cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b06d6ac110d5489fb4b795d628490167
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The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

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