Effect of Water-Pipe Smoking on the Normal Development of Zebrafish

Background: Among all types of tobacco consumption, Water-Pipe Smoking (WPS) is the most widely used in the Middle East and second-most in several other countries. The effect of WPS on normal development is not yet fully understood, thus the aim of this study is to explore the acute toxicity effects...

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Autores principales:	Zain Zaki Zakaria, Shaima Ahmad Aladwi, Fatiha Benslimane, Enas S. Al-Absi, Mashael Al-Shafai, Huseyin C. Yalcin, Ashraf Khalil, Ala-Eddin Al Moustafa, Maha Al-Asmakh
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	zebrafish waterpipe smoking toxicity testing developmental biology Medicine R
Acceso en línea:	https://doaj.org/article/b077a17f501b4f62b5806552a025df06
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spelling	oai:doaj.org-article:b077a17f501b4f62b5806552a025df062021-11-11T16:45:26ZEffect of Water-Pipe Smoking on the Normal Development of Zebrafish10.3390/ijerph1821116591660-46011661-7827https://doaj.org/article/b077a17f501b4f62b5806552a025df062021-11-01T00:00:00Zhttps://www.mdpi.com/1660-4601/18/21/11659https://doaj.org/toc/1661-7827https://doaj.org/toc/1660-4601Background: Among all types of tobacco consumption, Water-Pipe Smoking (WPS) is the most widely used in the Middle East and second-most in several other countries. The effect of WPS on normal development is not yet fully understood, thus the aim of this study is to explore the acute toxicity effects of WPS extract on zebrafish larvae. Methods: In this study, we compared the effects of WPS smoke condensates at concentrations varying from 50 to 200 µg/mL on developmental, cardiac, and behavioural (neurotoxicity) functions. Gene expression patterns of cardiac biomarkers were also evaluated by RT-qPCR. Results: Exposing zebrafish embryos to 50, 100, 150 and 200 µg/mL WPS for three days did not affect the normal morphology of Zebrafish embryos, as the tail flicking, behavioural and locomotion assays did not show any change. However, WPS deregulated cardiac markers including atrial natriuretic peptide (ANP/NPPA) and brain natriuretic peptide (BNP/NPPB). Furthermore, it induced apoptosis in a dose-dependent manner. Conclusion: Our data demonstrate that WPS can significantly affect specific cardiac parameters during the normal development of zebrafish. Further investigations are necessary to elucidate the pathogenic outcome of WPS on different aspects of human life, including pregnancy.Zain Zaki ZakariaShaima Ahmad AladwiFatiha BenslimaneEnas S. Al-AbsiMashael Al-ShafaiHuseyin C. YalcinAshraf KhalilAla-Eddin Al MoustafaMaha Al-AsmakhMDPI AGarticlezebrafishwaterpipe smokingtoxicity testingdevelopmental biologyMedicineRENInternational Journal of Environmental Research and Public Health, Vol 18, Iss 11659, p 11659 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zebrafish waterpipe smoking toxicity testing developmental biology Medicine R
spellingShingle	zebrafish waterpipe smoking toxicity testing developmental biology Medicine R Zain Zaki Zakaria Shaima Ahmad Aladwi Fatiha Benslimane Enas S. Al-Absi Mashael Al-Shafai Huseyin C. Yalcin Ashraf Khalil Ala-Eddin Al Moustafa Maha Al-Asmakh Effect of Water-Pipe Smoking on the Normal Development of Zebrafish
description	Background: Among all types of tobacco consumption, Water-Pipe Smoking (WPS) is the most widely used in the Middle East and second-most in several other countries. The effect of WPS on normal development is not yet fully understood, thus the aim of this study is to explore the acute toxicity effects of WPS extract on zebrafish larvae. Methods: In this study, we compared the effects of WPS smoke condensates at concentrations varying from 50 to 200 µg/mL on developmental, cardiac, and behavioural (neurotoxicity) functions. Gene expression patterns of cardiac biomarkers were also evaluated by RT-qPCR. Results: Exposing zebrafish embryos to 50, 100, 150 and 200 µg/mL WPS for three days did not affect the normal morphology of Zebrafish embryos, as the tail flicking, behavioural and locomotion assays did not show any change. However, WPS deregulated cardiac markers including atrial natriuretic peptide (ANP/NPPA) and brain natriuretic peptide (BNP/NPPB). Furthermore, it induced apoptosis in a dose-dependent manner. Conclusion: Our data demonstrate that WPS can significantly affect specific cardiac parameters during the normal development of zebrafish. Further investigations are necessary to elucidate the pathogenic outcome of WPS on different aspects of human life, including pregnancy.
format	article
author	Zain Zaki Zakaria Shaima Ahmad Aladwi Fatiha Benslimane Enas S. Al-Absi Mashael Al-Shafai Huseyin C. Yalcin Ashraf Khalil Ala-Eddin Al Moustafa Maha Al-Asmakh
author_facet	Zain Zaki Zakaria Shaima Ahmad Aladwi Fatiha Benslimane Enas S. Al-Absi Mashael Al-Shafai Huseyin C. Yalcin Ashraf Khalil Ala-Eddin Al Moustafa Maha Al-Asmakh
author_sort	Zain Zaki Zakaria
title	Effect of Water-Pipe Smoking on the Normal Development of Zebrafish
title_short	Effect of Water-Pipe Smoking on the Normal Development of Zebrafish
title_full	Effect of Water-Pipe Smoking on the Normal Development of Zebrafish
title_fullStr	Effect of Water-Pipe Smoking on the Normal Development of Zebrafish
title_full_unstemmed	Effect of Water-Pipe Smoking on the Normal Development of Zebrafish
title_sort	effect of water-pipe smoking on the normal development of zebrafish
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b077a17f501b4f62b5806552a025df06
work_keys_str_mv	AT zainzakizakaria effectofwaterpipesmokingonthenormaldevelopmentofzebrafish AT shaimaahmadaladwi effectofwaterpipesmokingonthenormaldevelopmentofzebrafish AT fatihabenslimane effectofwaterpipesmokingonthenormaldevelopmentofzebrafish AT enassalabsi effectofwaterpipesmokingonthenormaldevelopmentofzebrafish AT mashaelalshafai effectofwaterpipesmokingonthenormaldevelopmentofzebrafish AT huseyincyalcin effectofwaterpipesmokingonthenormaldevelopmentofzebrafish AT ashrafkhalil effectofwaterpipesmokingonthenormaldevelopmentofzebrafish AT alaeddinalmoustafa effectofwaterpipesmokingonthenormaldevelopmentofzebrafish AT mahaalasmakh effectofwaterpipesmokingonthenormaldevelopmentofzebrafish
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Effect of Water-Pipe Smoking on the Normal Development of Zebrafish

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