Horizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.

Horizontal gene transfer (HGT) is recognized as the major force for bacterial genome evolution. Yet, numerous questions remain about the transferred genes, their function, quantity and frequency. The extent to which genetic transformation by exogenous DNA has occurred over evolutionary time was init...

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Autores principales:	Saliou Fall, Anne Mercier, Franck Bertolla, Alexandra Calteau, Laurent Gueguen, Guy Perrière, Timothy M Vogel, Pascal Simonet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b07bf60064b3468d98b06f7e11a2e990
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spelling	oai:doaj.org-article:b07bf60064b3468d98b06f7e11a2e9902021-11-25T06:10:40ZHorizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.1932-620310.1371/journal.pone.0001055https://doaj.org/article/b07bf60064b3468d98b06f7e11a2e9902007-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001055https://doaj.org/toc/1932-6203Horizontal gene transfer (HGT) is recognized as the major force for bacterial genome evolution. Yet, numerous questions remain about the transferred genes, their function, quantity and frequency. The extent to which genetic transformation by exogenous DNA has occurred over evolutionary time was initially addressed by an in silico approach using the complete genome sequence of the Ralstonia solanacearum GMI1000 strain. Methods based on phylogenetic reconstruction of prokaryote homologous genes families detected 151 genes (13.3%) of foreign origin in the R. solanacearum genome and tentatively identified their bacterial origin. These putative transfers were analyzed in comparison to experimental transformation tests involving 18 different genomic DNA positions in the genome as sites for homologous or homeologous recombination. Significant transformation frequency differences were observed among these positions tested regardless of the overall genomic divergence of the R. solanacearum strains tested as recipients. The genomic positions containing the putative exogenous DNA were not systematically transformed at the highest frequencies. The two genomic "hot spots", which contain recA and mutS genes, exhibited transformation frequencies from 2 to more than 4 orders of magnitude higher than positions associated with other genes depending on the recipient strain. These results support the notion that the bacterial cell is equipped with active mechanisms to modulate acquisition of new DNA in different genomic positions. Bio-informatics study correlated recombination "hot-spots" to the presence of Chi-like signature sequences with which recombination might be preferentially initiated. The fundamental role of HGT is certainly not limited to the critical impact that the very rare foreign genes acquired mainly by chance can have on the bacterial adaptation potential. The frequency to which HGT with homologous and homeologous DNA happens in the environment might have led the bacteria to hijack DNA repair mechanisms in order to generate genetic diversity without losing too much genomic stability.Saliou FallAnne MercierFranck BertollaAlexandra CalteauLaurent GueguenGuy PerrièreTimothy M VogelPascal SimonetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 10, p e1055 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saliou Fall Anne Mercier Franck Bertolla Alexandra Calteau Laurent Gueguen Guy Perrière Timothy M Vogel Pascal Simonet Horizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.
description	Horizontal gene transfer (HGT) is recognized as the major force for bacterial genome evolution. Yet, numerous questions remain about the transferred genes, their function, quantity and frequency. The extent to which genetic transformation by exogenous DNA has occurred over evolutionary time was initially addressed by an in silico approach using the complete genome sequence of the Ralstonia solanacearum GMI1000 strain. Methods based on phylogenetic reconstruction of prokaryote homologous genes families detected 151 genes (13.3%) of foreign origin in the R. solanacearum genome and tentatively identified their bacterial origin. These putative transfers were analyzed in comparison to experimental transformation tests involving 18 different genomic DNA positions in the genome as sites for homologous or homeologous recombination. Significant transformation frequency differences were observed among these positions tested regardless of the overall genomic divergence of the R. solanacearum strains tested as recipients. The genomic positions containing the putative exogenous DNA were not systematically transformed at the highest frequencies. The two genomic "hot spots", which contain recA and mutS genes, exhibited transformation frequencies from 2 to more than 4 orders of magnitude higher than positions associated with other genes depending on the recipient strain. These results support the notion that the bacterial cell is equipped with active mechanisms to modulate acquisition of new DNA in different genomic positions. Bio-informatics study correlated recombination "hot-spots" to the presence of Chi-like signature sequences with which recombination might be preferentially initiated. The fundamental role of HGT is certainly not limited to the critical impact that the very rare foreign genes acquired mainly by chance can have on the bacterial adaptation potential. The frequency to which HGT with homologous and homeologous DNA happens in the environment might have led the bacteria to hijack DNA repair mechanisms in order to generate genetic diversity without losing too much genomic stability.
format	article
author	Saliou Fall Anne Mercier Franck Bertolla Alexandra Calteau Laurent Gueguen Guy Perrière Timothy M Vogel Pascal Simonet
author_facet	Saliou Fall Anne Mercier Franck Bertolla Alexandra Calteau Laurent Gueguen Guy Perrière Timothy M Vogel Pascal Simonet
author_sort	Saliou Fall
title	Horizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.
title_short	Horizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.
title_full	Horizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.
title_fullStr	Horizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.
title_full_unstemmed	Horizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.
title_sort	horizontal gene transfer regulation in bacteria as a "spandrel" of dna repair mechanisms.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/b07bf60064b3468d98b06f7e11a2e990
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Horizontal gene transfer regulation in bacteria as a "spandrel" of DNA repair mechanisms.

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