Identification of circRNA_001846 as putative non–small cell lung cancer biomarker

CircRNAs play diverse roles in the regulation of oncogenic processes, yet the roles of these circRNAs in non–small cell lung cancer (NSCLC) remain to be fully clarified. Herein, patterns of circRNA expression in NSCLC tissues and paracancerous tissues were assessed, and the relationships between the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fan Yang, Chunlan Ma, Jing Qiu, Xiaoli Feng, Kai Yang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	circrna circrna_001846 prognosis biomarker nsclc Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/b088374f018a413bafa12e55c609d545
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b088374f018a413bafa12e55c609d545
record_format	dspace
spelling	oai:doaj.org-article:b088374f018a413bafa12e55c609d5452021-11-04T15:51:54ZIdentification of circRNA_001846 as putative non–small cell lung cancer biomarker2165-59792165-598710.1080/21655979.2021.1991161https://doaj.org/article/b088374f018a413bafa12e55c609d5452021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1991161https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987CircRNAs play diverse roles in the regulation of oncogenic processes, yet the roles of these circRNAs in non–small cell lung cancer (NSCLC) remain to be fully clarified. Herein, patterns of circRNA expression in NSCLC tissues and paracancerous tissues were assessed, and the relationships between these circRNAs and NSCLC patient clinical findings were assessed. NSCLC tissues were evaluated using a circRNA microarray approach, with Quantitative real‑time polymerase chain reaction (qPCR) qPCR being used to validate candidate circRNA expression levels in NSCLC patients peripheral blood samples. GEO2R was used to analyze the array data. SPSS23.0, GraphPad Prism, and Sigmaplot were utilized for statistical analyses. Overall, 114 circRNAs that were differentially expressed in NSCLC tissue relative to paracancerous tissue levels were identified, of which 77 and 37 were downregulated and upregulated, respectively. CircRNA_001846 were then chosen based on its differential expression score. Consistent with array findings, serum samples from NSCLC patients exhibited circRNA_001846 upregulation relative to those from healthy individuals. The circRNA_001846 was associated with tumor differentiation, lymph node metastasis, and node metastasis stage. Receiver operating characteristic (ROC) curves analyses revealed that levels of circRNA_001846 in the serum were capable of differentiating between individuals diagnosed with NSCLC and controls at a cut off of 3.9496, yielding respective sensitivity and specificity values of 78.2% and 81.1%, with an area under the curve (AUC) value of 0.872. When combined with carcinoembryonic antigen (CEA), this circRNA achieved an improved AUC value of 0.925. CircRNA_001846 may represent a promising diagnostic biomarker for NSCLC.Fan YangChunlan MaJing QiuXiaoli FengKai YangTaylor & Francis Grouparticlecircrnacircrna_001846prognosis biomarkernsclcBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8690-8697 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circrna circrna_001846 prognosis biomarker nsclc Biotechnology TP248.13-248.65
spellingShingle	circrna circrna_001846 prognosis biomarker nsclc Biotechnology TP248.13-248.65 Fan Yang Chunlan Ma Jing Qiu Xiaoli Feng Kai Yang Identification of circRNA_001846 as putative non–small cell lung cancer biomarker
description	CircRNAs play diverse roles in the regulation of oncogenic processes, yet the roles of these circRNAs in non–small cell lung cancer (NSCLC) remain to be fully clarified. Herein, patterns of circRNA expression in NSCLC tissues and paracancerous tissues were assessed, and the relationships between these circRNAs and NSCLC patient clinical findings were assessed. NSCLC tissues were evaluated using a circRNA microarray approach, with Quantitative real‑time polymerase chain reaction (qPCR) qPCR being used to validate candidate circRNA expression levels in NSCLC patients peripheral blood samples. GEO2R was used to analyze the array data. SPSS23.0, GraphPad Prism, and Sigmaplot were utilized for statistical analyses. Overall, 114 circRNAs that were differentially expressed in NSCLC tissue relative to paracancerous tissue levels were identified, of which 77 and 37 were downregulated and upregulated, respectively. CircRNA_001846 were then chosen based on its differential expression score. Consistent with array findings, serum samples from NSCLC patients exhibited circRNA_001846 upregulation relative to those from healthy individuals. The circRNA_001846 was associated with tumor differentiation, lymph node metastasis, and node metastasis stage. Receiver operating characteristic (ROC) curves analyses revealed that levels of circRNA_001846 in the serum were capable of differentiating between individuals diagnosed with NSCLC and controls at a cut off of 3.9496, yielding respective sensitivity and specificity values of 78.2% and 81.1%, with an area under the curve (AUC) value of 0.872. When combined with carcinoembryonic antigen (CEA), this circRNA achieved an improved AUC value of 0.925. CircRNA_001846 may represent a promising diagnostic biomarker for NSCLC.
format	article
author	Fan Yang Chunlan Ma Jing Qiu Xiaoli Feng Kai Yang
author_facet	Fan Yang Chunlan Ma Jing Qiu Xiaoli Feng Kai Yang
author_sort	Fan Yang
title	Identification of circRNA_001846 as putative non–small cell lung cancer biomarker
title_short	Identification of circRNA_001846 as putative non–small cell lung cancer biomarker
title_full	Identification of circRNA_001846 as putative non–small cell lung cancer biomarker
title_fullStr	Identification of circRNA_001846 as putative non–small cell lung cancer biomarker
title_full_unstemmed	Identification of circRNA_001846 as putative non–small cell lung cancer biomarker
title_sort	identification of circrna_001846 as putative non–small cell lung cancer biomarker
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/b088374f018a413bafa12e55c609d545
work_keys_str_mv	AT fanyang identificationofcircrna001846asputativenonsmallcelllungcancerbiomarker AT chunlanma identificationofcircrna001846asputativenonsmallcelllungcancerbiomarker AT jingqiu identificationofcircrna001846asputativenonsmallcelllungcancerbiomarker AT xiaolifeng identificationofcircrna001846asputativenonsmallcelllungcancerbiomarker AT kaiyang identificationofcircrna001846asputativenonsmallcelllungcancerbiomarker
_version_	1718444734719983616

Identification of circRNA_001846 as putative non–small cell lung cancer biomarker

Ejemplares similares