Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata

Abstract Background Sucrose (Suc) hydrolysis is directly associated with plants tolerance to multiple abiotic stresses. Invertase (INV) enzymes irreversibly catalyze Suc degradation to produce glucose (Glc) and fructose (Frc). However, genome-wide identification and function of individual members of...

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Autores principales:	Bachar Dahro, Yue Wang, Ahmed Alhag, Chunlong Li, Dayong Guo, Ji-Hong Liu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	P. trifoliata Invertase Genome-wide identification Sucrose hydrolysis Gene expression Abiotic stresses Botany QK1-989
Acceso en línea:	https://doaj.org/article/b08e2554dc744152963dd35177163b6a
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spelling	oai:doaj.org-article:b08e2554dc744152963dd35177163b6a2021-11-28T12:07:19ZGenome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata10.1186/s12870-021-03337-31471-2229https://doaj.org/article/b08e2554dc744152963dd35177163b6a2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03337-3https://doaj.org/toc/1471-2229Abstract Background Sucrose (Suc) hydrolysis is directly associated with plants tolerance to multiple abiotic stresses. Invertase (INV) enzymes irreversibly catalyze Suc degradation to produce glucose (Glc) and fructose (Frc). However, genome-wide identification and function of individual members of the INV gene family in Poncirus trifoliata or its Citrus relatives in response to abiotic stresses are not fully understood. Results In this report, fourteen non-redundant PtrINV family members were identified in P. trifoliata including seven alkaline/neutral INV genes (PtrA/NINV1–7), two vacuolar INV genes (PtrVINV1–2), and five cell wall INV isoforms (PtrCWINV1–5). A comprehensive analysis based on the biochemical characteristics, the chromosomal location, the exon–intron structures and the evolutionary relationships demonstrated the conservation and the divergence of PtrINVs. In addition, expression analysis of INV genes during several abiotic stresses in various tissues indicated the central role of A/NINV7 among INV family members in response to abiotic stresses. Furthermore, our data demonstrated that high accumulation of Suc, Glc, Frc and total sugar contents were directly correlated with the elevated activities of soluble INV enzymes in the cold-tolerant P. trifoliata, C. ichangensis and C. sinensis, demonstrating the potential role of soluble INV enzymes for the cold tolerance of Citrus. Conclusions This work offered a framework for understanding the physiological role of INV genes and laid a foundation for future functional studies of these genes in response to abiotic stresses.Bachar DahroYue WangAhmed AlhagChunlong LiDayong GuoJi-Hong LiuBMCarticleP. trifoliataInvertaseGenome-wide identificationSucrose hydrolysisGene expressionAbiotic stressesBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	P. trifoliata Invertase Genome-wide identification Sucrose hydrolysis Gene expression Abiotic stresses Botany QK1-989
spellingShingle	P. trifoliata Invertase Genome-wide identification Sucrose hydrolysis Gene expression Abiotic stresses Botany QK1-989 Bachar Dahro Yue Wang Ahmed Alhag Chunlong Li Dayong Guo Ji-Hong Liu Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata
description	Abstract Background Sucrose (Suc) hydrolysis is directly associated with plants tolerance to multiple abiotic stresses. Invertase (INV) enzymes irreversibly catalyze Suc degradation to produce glucose (Glc) and fructose (Frc). However, genome-wide identification and function of individual members of the INV gene family in Poncirus trifoliata or its Citrus relatives in response to abiotic stresses are not fully understood. Results In this report, fourteen non-redundant PtrINV family members were identified in P. trifoliata including seven alkaline/neutral INV genes (PtrA/NINV1–7), two vacuolar INV genes (PtrVINV1–2), and five cell wall INV isoforms (PtrCWINV1–5). A comprehensive analysis based on the biochemical characteristics, the chromosomal location, the exon–intron structures and the evolutionary relationships demonstrated the conservation and the divergence of PtrINVs. In addition, expression analysis of INV genes during several abiotic stresses in various tissues indicated the central role of A/NINV7 among INV family members in response to abiotic stresses. Furthermore, our data demonstrated that high accumulation of Suc, Glc, Frc and total sugar contents were directly correlated with the elevated activities of soluble INV enzymes in the cold-tolerant P. trifoliata, C. ichangensis and C. sinensis, demonstrating the potential role of soluble INV enzymes for the cold tolerance of Citrus. Conclusions This work offered a framework for understanding the physiological role of INV genes and laid a foundation for future functional studies of these genes in response to abiotic stresses.
format	article
author	Bachar Dahro Yue Wang Ahmed Alhag Chunlong Li Dayong Guo Ji-Hong Liu
author_facet	Bachar Dahro Yue Wang Ahmed Alhag Chunlong Li Dayong Guo Ji-Hong Liu
author_sort	Bachar Dahro
title	Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata
title_short	Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata
title_full	Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata
title_fullStr	Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata
title_full_unstemmed	Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata
title_sort	genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in poncirus trifoliata
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b08e2554dc744152963dd35177163b6a
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_version_	1718408219785691136

Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata

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