Rhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial

Abstract Rhamnan sulphate (RS), a sulphated polysaccharide from Monostroma nitidum, possesses several biological properties that help in treating diseases such as viral infection, thrombosis, and obesity. In the present study, we first administered RS (0.25 mg/g food volume) orally to high-fat diet-...

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Main Authors:	Yasuhito Shimada, Masahiro Terasawa, Fumiyoshi Okazaki, Hiroko Nakayama, Liqing Zang, Kaoru Nishiura, Koichi Matsuda, Norihiro Nishimura
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/b08e620e011c43188f8d2dc63f8f74ec
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spelling	oai:doaj.org-article:b08e620e011c43188f8d2dc63f8f74ec2021-12-02T18:34:20ZRhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial10.1038/s41598-021-92459-72045-2322https://doaj.org/article/b08e620e011c43188f8d2dc63f8f74ec2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92459-7https://doaj.org/toc/2045-2322Abstract Rhamnan sulphate (RS), a sulphated polysaccharide from Monostroma nitidum, possesses several biological properties that help in treating diseases such as viral infection, thrombosis, and obesity. In the present study, we first administered RS (0.25 mg/g food volume) orally to high-fat diet-treated mice for 4 weeks. RS increased the faecal volume and calorie excretion with decreased plasma lipids, which was in accordance with the results of our previous zebrafish study. Notably, as the excretion amount by RS increased in the mice, we hypothesised that RS could decrease the chance of constipation in mice and also in human subjects because RS is considered as a dietary fibre. We administrated RS (100 mg/day) to subjects with low defaecation frequencies (3–5 times/week) for 2 weeks in double-blind placebo-controlled manner. As a result, RS administration significantly increased the frequency of dejection without any side effects, although no effect was observed on the body weight and blood lipids. Moreover, we performed 16s rRNA-seq analysis of the gut microbiota in these subjects. Metagenomics profiling using PICRUSt revealed functional alternation of the KEGG pathways, which could be involved in the therapeutic effect of RS for constipation.Yasuhito ShimadaMasahiro TerasawaFumiyoshi OkazakiHiroko NakayamaLiqing ZangKaoru NishiuraKoichi MatsudaNorihiro NishimuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yasuhito Shimada Masahiro Terasawa Fumiyoshi Okazaki Hiroko Nakayama Liqing Zang Kaoru Nishiura Koichi Matsuda Norihiro Nishimura Rhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial
description	Abstract Rhamnan sulphate (RS), a sulphated polysaccharide from Monostroma nitidum, possesses several biological properties that help in treating diseases such as viral infection, thrombosis, and obesity. In the present study, we first administered RS (0.25 mg/g food volume) orally to high-fat diet-treated mice for 4 weeks. RS increased the faecal volume and calorie excretion with decreased plasma lipids, which was in accordance with the results of our previous zebrafish study. Notably, as the excretion amount by RS increased in the mice, we hypothesised that RS could decrease the chance of constipation in mice and also in human subjects because RS is considered as a dietary fibre. We administrated RS (100 mg/day) to subjects with low defaecation frequencies (3–5 times/week) for 2 weeks in double-blind placebo-controlled manner. As a result, RS administration significantly increased the frequency of dejection without any side effects, although no effect was observed on the body weight and blood lipids. Moreover, we performed 16s rRNA-seq analysis of the gut microbiota in these subjects. Metagenomics profiling using PICRUSt revealed functional alternation of the KEGG pathways, which could be involved in the therapeutic effect of RS for constipation.
format	article
author	Yasuhito Shimada Masahiro Terasawa Fumiyoshi Okazaki Hiroko Nakayama Liqing Zang Kaoru Nishiura Koichi Matsuda Norihiro Nishimura
author_facet	Yasuhito Shimada Masahiro Terasawa Fumiyoshi Okazaki Hiroko Nakayama Liqing Zang Kaoru Nishiura Koichi Matsuda Norihiro Nishimura
author_sort	Yasuhito Shimada
title	Rhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial
title_short	Rhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial
title_full	Rhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial
title_fullStr	Rhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial
title_full_unstemmed	Rhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial
title_sort	rhamnan sulphate from green algae monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b08e620e011c43188f8d2dc63f8f74ec
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Rhamnan sulphate from green algae Monostroma nitidum improves constipation with gut microbiome alteration in double-blind placebo-controlled trial

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