Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake

Abstract Lactobacillus delbrueckii JCM 1002T grows on highly polymerized inulin-type fructans as its sole carbon source. When it was grown on inulin, a > 10 kb long gene cluster inuABCDEF (Ldb1381-1386) encoding a plausible ABC transporter was suggested to be induced, since a transcriptome analys...

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Autores principales:	Yuji Tsujikawa, Shu Ishikawa, Iwao Sakane, Ken-ichi Yoshida, Ro Osawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b090aa418e4b49998249eb3f16014a9e
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spelling	oai:doaj.org-article:b090aa418e4b49998249eb3f16014a9e2021-12-02T17:06:09ZIdentification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake10.1038/s41598-021-95356-12045-2322https://doaj.org/article/b090aa418e4b49998249eb3f16014a9e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95356-1https://doaj.org/toc/2045-2322Abstract Lactobacillus delbrueckii JCM 1002T grows on highly polymerized inulin-type fructans as its sole carbon source. When it was grown on inulin, a > 10 kb long gene cluster inuABCDEF (Ldb1381-1386) encoding a plausible ABC transporter was suggested to be induced, since a transcriptome analysis revealed that the fourth gene inuD (Ldb1384) was up-regulated most prominently. Although Bacillus subtilis 168 is originally unable to utilize inulin, it became to grow on inulin upon heterologous expression of inuABCDEF. When freshly cultured cells of the recombinant B. subtilis were then densely suspended in buffer containing inulin polymers and incubated, inulin gradually disappeared from the buffer and accumulated in the cells without being degraded, whereas levan-type fructans did not disappear. The results imply that inuABCDEF might encode a novel ABC transporter in L. delbrueckii to “monopolize” inulin polymers selectively, thereby, providing a possible advantage in competition with other concomitant inulin-utilizing bacteria.Yuji TsujikawaShu IshikawaIwao SakaneKen-ichi YoshidaRo OsawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuji Tsujikawa Shu Ishikawa Iwao Sakane Ken-ichi Yoshida Ro Osawa Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake
description	Abstract Lactobacillus delbrueckii JCM 1002T grows on highly polymerized inulin-type fructans as its sole carbon source. When it was grown on inulin, a > 10 kb long gene cluster inuABCDEF (Ldb1381-1386) encoding a plausible ABC transporter was suggested to be induced, since a transcriptome analysis revealed that the fourth gene inuD (Ldb1384) was up-regulated most prominently. Although Bacillus subtilis 168 is originally unable to utilize inulin, it became to grow on inulin upon heterologous expression of inuABCDEF. When freshly cultured cells of the recombinant B. subtilis were then densely suspended in buffer containing inulin polymers and incubated, inulin gradually disappeared from the buffer and accumulated in the cells without being degraded, whereas levan-type fructans did not disappear. The results imply that inuABCDEF might encode a novel ABC transporter in L. delbrueckii to “monopolize” inulin polymers selectively, thereby, providing a possible advantage in competition with other concomitant inulin-utilizing bacteria.
format	article
author	Yuji Tsujikawa Shu Ishikawa Iwao Sakane Ken-ichi Yoshida Ro Osawa
author_facet	Yuji Tsujikawa Shu Ishikawa Iwao Sakane Ken-ichi Yoshida Ro Osawa
author_sort	Yuji Tsujikawa
title	Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake
title_short	Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake
title_full	Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake
title_fullStr	Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake
title_full_unstemmed	Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake
title_sort	identification of genes encoding a novel abc transporter in lactobacillus delbrueckii for inulin polymers uptake
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b090aa418e4b49998249eb3f16014a9e
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_version_	1718381751224500224

Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake

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