Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis

Various agricultural products used in food fermentation are polluted by heavy metals, especially copper, which seriously endangers human health. Methods to remove copper with microbial strategies have gained interests. A novel Meyerozyma guilliermondii GXDK6 could survive independently under high st...

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Autores principales:	Ru Bu, Bing Yan, Huijie Sun, Mengcheng Zhou, Huashan Bai, Xinghua Cai, Xueyan Mo, Guijiao Su, Chengjian Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	M. guilliermondii copper tolerance mechanism antioxidant enzyme genes integrated OMICs analysis non-enzymatic antioxidant genes Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b0917771f01a4ae0805740e23628370f
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spelling	oai:doaj.org-article:b0917771f01a4ae0805740e23628370f2021-11-18T08:18:19ZCopper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis1664-302X10.3389/fmicb.2021.771878https://doaj.org/article/b0917771f01a4ae0805740e23628370f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.771878/fullhttps://doaj.org/toc/1664-302XVarious agricultural products used in food fermentation are polluted by heavy metals, especially copper, which seriously endangers human health. Methods to remove copper with microbial strategies have gained interests. A novel Meyerozyma guilliermondii GXDK6 could survive independently under high stress of copper (1400 ppm). The copper tolerance mechanism of GXDK6 was revealed by integrated omics in this work. Whole-genome analysis showed that nine genes (i.e., CCC2, CTR3, FRE2, GGT, GST, CAT, SOD2, PXMP4, and HSP82) were related to GXDK6 copper tolerance. Copper stress elevated glutathione metabolism-related gene expression, glutathione content, and glutathione sulfur transferase activity, suggesting enhanced copper conjugation and detoxification in cells. The inhibited copper uptake by Ctr3 and enhanced copper efflux by Ccc2 contributed to the decrease in intracellular copper concentration. The improved expression of antioxidant enzyme genes (PXMP4, SOD2, and CAT), accompanied by the enhanced activities of antioxidant enzymes (peroxidase, superoxide dismutase, and catalase), decreased copper-induced reactive oxygen species production, protein carbonylation, lipid peroxidation, and cell death. The metabolite D-mannose against harsh stress conditions was beneficial to improving copper tolerance. This study contributed to understanding the copper tolerance mechanism of M. guilliermondii and its application in removing copper during fermentation.Ru BuBing YanHuijie SunMengcheng ZhouHuashan BaiXinghua CaiXueyan MoGuijiao SuChengjian JiangChengjian JiangFrontiers Media S.A.articleM. guilliermondiicopper tolerance mechanismantioxidant enzyme genesintegrated OMICs analysisnon-enzymatic antioxidant genesMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	M. guilliermondii copper tolerance mechanism antioxidant enzyme genes integrated OMICs analysis non-enzymatic antioxidant genes Microbiology QR1-502
spellingShingle	M. guilliermondii copper tolerance mechanism antioxidant enzyme genes integrated OMICs analysis non-enzymatic antioxidant genes Microbiology QR1-502 Ru Bu Bing Yan Huijie Sun Mengcheng Zhou Huashan Bai Xinghua Cai Xueyan Mo Guijiao Su Chengjian Jiang Chengjian Jiang Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis
description	Various agricultural products used in food fermentation are polluted by heavy metals, especially copper, which seriously endangers human health. Methods to remove copper with microbial strategies have gained interests. A novel Meyerozyma guilliermondii GXDK6 could survive independently under high stress of copper (1400 ppm). The copper tolerance mechanism of GXDK6 was revealed by integrated omics in this work. Whole-genome analysis showed that nine genes (i.e., CCC2, CTR3, FRE2, GGT, GST, CAT, SOD2, PXMP4, and HSP82) were related to GXDK6 copper tolerance. Copper stress elevated glutathione metabolism-related gene expression, glutathione content, and glutathione sulfur transferase activity, suggesting enhanced copper conjugation and detoxification in cells. The inhibited copper uptake by Ctr3 and enhanced copper efflux by Ccc2 contributed to the decrease in intracellular copper concentration. The improved expression of antioxidant enzyme genes (PXMP4, SOD2, and CAT), accompanied by the enhanced activities of antioxidant enzymes (peroxidase, superoxide dismutase, and catalase), decreased copper-induced reactive oxygen species production, protein carbonylation, lipid peroxidation, and cell death. The metabolite D-mannose against harsh stress conditions was beneficial to improving copper tolerance. This study contributed to understanding the copper tolerance mechanism of M. guilliermondii and its application in removing copper during fermentation.
format	article
author	Ru Bu Bing Yan Huijie Sun Mengcheng Zhou Huashan Bai Xinghua Cai Xueyan Mo Guijiao Su Chengjian Jiang Chengjian Jiang
author_facet	Ru Bu Bing Yan Huijie Sun Mengcheng Zhou Huashan Bai Xinghua Cai Xueyan Mo Guijiao Su Chengjian Jiang Chengjian Jiang
author_sort	Ru Bu
title	Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis
title_short	Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis
title_full	Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis
title_fullStr	Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis
title_full_unstemmed	Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis
title_sort	copper tolerance mechanism of the novel marine multi-stress tolerant yeast meyerozyma guilliermondii gxdk6 as revealed by integrated omics analysis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b0917771f01a4ae0805740e23628370f
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Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis

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