Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.

A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derive...

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Autores principales:	Emmanuel Bréard, Estelle Lara, Loïc Comtet, Cyril Viarouge, Virginie Doceul, Alexandra Desprat, Damien Vitour, Nathalie Pozzi, Ann Brigitte Cay, Nick De Regge, Philippe Pourquier, Horst Schirrmeier, Bernd Hoffmann, Martin Beer, Corinne Sailleau, Stéphan Zientara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0964c08c56347c2ba6fc11771f8c196
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spelling	oai:doaj.org-article:b0964c08c56347c2ba6fc11771f8c1962021-11-18T08:01:26ZValidation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.1932-620310.1371/journal.pone.0053446https://doaj.org/article/b0964c08c56347c2ba6fc11771f8c1962013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23335964/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.Emmanuel BréardEstelle LaraLoïc ComtetCyril ViarougeVirginie DoceulAlexandra DespratDamien VitourNathalie PozziAnn Brigitte CayNick De ReggePhilippe PourquierHorst SchirrmeierBernd HoffmannMartin BeerCorinne SailleauStéphan ZientaraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53446 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emmanuel Bréard Estelle Lara Loïc Comtet Cyril Viarouge Virginie Doceul Alexandra Desprat Damien Vitour Nathalie Pozzi Ann Brigitte Cay Nick De Regge Philippe Pourquier Horst Schirrmeier Bernd Hoffmann Martin Beer Corinne Sailleau Stéphan Zientara Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
description	A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.
format	article
author	Emmanuel Bréard Estelle Lara Loïc Comtet Cyril Viarouge Virginie Doceul Alexandra Desprat Damien Vitour Nathalie Pozzi Ann Brigitte Cay Nick De Regge Philippe Pourquier Horst Schirrmeier Bernd Hoffmann Martin Beer Corinne Sailleau Stéphan Zientara
author_facet	Emmanuel Bréard Estelle Lara Loïc Comtet Cyril Viarouge Virginie Doceul Alexandra Desprat Damien Vitour Nathalie Pozzi Ann Brigitte Cay Nick De Regge Philippe Pourquier Horst Schirrmeier Bernd Hoffmann Martin Beer Corinne Sailleau Stéphan Zientara
author_sort	Emmanuel Bréard
title	Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
title_short	Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
title_full	Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
title_fullStr	Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
title_full_unstemmed	Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
title_sort	validation of a commercially available indirect elisa using a nucleocapside recombinant protein for detection of schmallenberg virus antibodies.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b0964c08c56347c2ba6fc11771f8c196
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Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.

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