Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi

Abstract Chagas disease is caused by Trypanosoma cruzi and affects thousands of people. Drugs currently used in therapy are toxic and have therapeutic limitations. In addition, the genetic diversity of T. cruzi represents an important variable and challenge in treatment. Sodium diethyldithiocarbamat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Johny Wysllas de Freitas Oliveira, Taffarel Melo Torres, Cláudia Jassica Gonçalves Moreno, Bruno Amorim-Carmo, Igor Zumba Damasceno, Ana Katarina Menezes Cruz Soares, Jefferson da Silva Barbosa, Hugo Alexandre Oliveira Rocha, Marcelo Sousa Silva
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0a03aed4c164be18941f3580310a16c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b0a03aed4c164be18941f3580310a16c
record_format	dspace
spelling	oai:doaj.org-article:b0a03aed4c164be18941f3580310a16c2021-12-02T15:49:39ZInsights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi10.1038/s41598-021-90719-02045-2322https://doaj.org/article/b0a03aed4c164be18941f3580310a16c2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90719-0https://doaj.org/toc/2045-2322Abstract Chagas disease is caused by Trypanosoma cruzi and affects thousands of people. Drugs currently used in therapy are toxic and have therapeutic limitations. In addition, the genetic diversity of T. cruzi represents an important variable and challenge in treatment. Sodium diethyldithiocarbamate (DETC) is a compound with pharmacological versatility acting as metal chelators and ROS generation. Thus, the objective was to characterize the antiparasitic action of DETC against different strains and forms of T. cruzi and their mechanism. The different strains of T. cruzi were grown in LIT medium. To evaluate the antiparasitic activity of DETC, epimastigote and trypomastigote forms of T. cruzi were used by resazurin reduction methods and by counting. Different response patterns were obtained between the strains and an IC50 of DETC ranging from 9.44 ± 3,181 to 60.49 ± 7.62 µM. Cell cytotoxicity against 3T3 and RAW cell lines and evaluated by MTT, demonstrated that DETC in high concentration (2222.00 µM) presents low toxicity. Yet, DETC causes mitochondrial damage in T. cruzi, as well as disruption in parasite membrane. DETC has antiparasitic activity against different genotypes and forms of T. cruzi, therefore, representing a promising molecule as a drug for the treatment of Chagas disease.Johny Wysllas de Freitas OliveiraTaffarel Melo TorresCláudia Jassica Gonçalves MorenoBruno Amorim-CarmoIgor Zumba DamascenoAna Katarina Menezes Cruz SoaresJefferson da Silva BarbosaHugo Alexandre Oliveira RochaMarcelo Sousa SilvaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johny Wysllas de Freitas Oliveira Taffarel Melo Torres Cláudia Jassica Gonçalves Moreno Bruno Amorim-Carmo Igor Zumba Damasceno Ana Katarina Menezes Cruz Soares Jefferson da Silva Barbosa Hugo Alexandre Oliveira Rocha Marcelo Sousa Silva Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi
description	Abstract Chagas disease is caused by Trypanosoma cruzi and affects thousands of people. Drugs currently used in therapy are toxic and have therapeutic limitations. In addition, the genetic diversity of T. cruzi represents an important variable and challenge in treatment. Sodium diethyldithiocarbamate (DETC) is a compound with pharmacological versatility acting as metal chelators and ROS generation. Thus, the objective was to characterize the antiparasitic action of DETC against different strains and forms of T. cruzi and their mechanism. The different strains of T. cruzi were grown in LIT medium. To evaluate the antiparasitic activity of DETC, epimastigote and trypomastigote forms of T. cruzi were used by resazurin reduction methods and by counting. Different response patterns were obtained between the strains and an IC50 of DETC ranging from 9.44 ± 3,181 to 60.49 ± 7.62 µM. Cell cytotoxicity against 3T3 and RAW cell lines and evaluated by MTT, demonstrated that DETC in high concentration (2222.00 µM) presents low toxicity. Yet, DETC causes mitochondrial damage in T. cruzi, as well as disruption in parasite membrane. DETC has antiparasitic activity against different genotypes and forms of T. cruzi, therefore, representing a promising molecule as a drug for the treatment of Chagas disease.
format	article
author	Johny Wysllas de Freitas Oliveira Taffarel Melo Torres Cláudia Jassica Gonçalves Moreno Bruno Amorim-Carmo Igor Zumba Damasceno Ana Katarina Menezes Cruz Soares Jefferson da Silva Barbosa Hugo Alexandre Oliveira Rocha Marcelo Sousa Silva
author_facet	Johny Wysllas de Freitas Oliveira Taffarel Melo Torres Cláudia Jassica Gonçalves Moreno Bruno Amorim-Carmo Igor Zumba Damasceno Ana Katarina Menezes Cruz Soares Jefferson da Silva Barbosa Hugo Alexandre Oliveira Rocha Marcelo Sousa Silva
author_sort	Johny Wysllas de Freitas Oliveira
title	Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi
title_short	Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi
title_full	Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi
title_fullStr	Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi
title_full_unstemmed	Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi
title_sort	insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of trypanosoma cruzi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0a03aed4c164be18941f3580310a16c
work_keys_str_mv	AT johnywysllasdefreitasoliveira insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi AT taffarelmelotorres insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi AT claudiajassicagoncalvesmoreno insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi AT brunoamorimcarmo insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi AT igorzumbadamasceno insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi AT anakatarinamenezescruzsoares insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi AT jeffersondasilvabarbosa insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi AT hugoalexandreoliveirarocha insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi AT marcelosousasilva insightsofantiparasiticactivityofsodiumdiethyldithiocarbamateagainstdifferentstrainsoftrypanosomacruzi
_version_	1718385712416423936

Insights of antiparasitic activity of sodium diethyldithiocarbamate against different strains of Trypanosoma cruzi

Ejemplares similares