Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model

Omega-3 polyunsaturated fatty acids (PUFA n3) ameliorate inflammation in different diseases and potentially improve neurological function after neuronal injury. Following spinal cord injury (SCI), inflammatory events result in caspase-1 mediated activation of interleukin-1 beta (IL-1b) and 18. We ai...

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Autores principales:	Maryam Baazm, Victoria Behrens, Cordian Beyer, Omid Nikoubashman, Adib Zendedel
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	omega-3 fatty acids spinal cord injury inflammasome Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b0a25addda534cffa7ec58a326d4aa03
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spelling	oai:doaj.org-article:b0a25addda534cffa7ec58a326d4aa032021-11-25T17:11:58ZRegulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model10.3390/cells101131472073-4409https://doaj.org/article/b0a25addda534cffa7ec58a326d4aa032021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3147https://doaj.org/toc/2073-4409Omega-3 polyunsaturated fatty acids (PUFA n3) ameliorate inflammation in different diseases and potentially improve neurological function after neuronal injury. Following spinal cord injury (SCI), inflammatory events result in caspase-1 mediated activation of interleukin-1 beta (IL-1b) and 18. We aim to evaluate the neuroprotective potency of PUFA n3 in suppressing the formation and activation of inflammasomes following SCI. Male Wistar rats were divided into four groups: control, SCI, SCI+PUFA n3, and SCI+Lipofundin MCT (medium-chain triglyceride; vehicle). PUFA n3 or vehicle was intravenously administered immediately after SCI and every 24 h for the next three days. We analyzed the expression of NLRP3, NLRP1, ASC, caspase-1, IL-1b, and 18 in the spinal cord. The distribution of microglia, oligodendrocytes, and astrocytes was assessed by immunohistochemistry analysis. Behavioral testing showed significantly improved locomotor recovery in PUFA n3-treated animals and the SCI-induced upregulation of inflammasome components was reduced. Histopathological evaluation confirmed the suppression of microgliosis, increased numbers of oligodendrocytes, and the prevention of demyelination by PUFA n3. Our data support the neuroprotective role of PUFA n3 by targeting the NLRP3 inflammasome. These findings provide evidence that PUFA n3 has therapeutic effects which potentially attenuate neuronal damage in SCI and possibly also in other neuronal injuries.Maryam BaazmVictoria BehrensCordian BeyerOmid NikoubashmanAdib ZendedelMDPI AGarticleomega-3 fatty acidsspinal cord injuryinflammasomeBiology (General)QH301-705.5ENCells, Vol 10, Iss 3147, p 3147 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	omega-3 fatty acids spinal cord injury inflammasome Biology (General) QH301-705.5
spellingShingle	omega-3 fatty acids spinal cord injury inflammasome Biology (General) QH301-705.5 Maryam Baazm Victoria Behrens Cordian Beyer Omid Nikoubashman Adib Zendedel Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model
description	Omega-3 polyunsaturated fatty acids (PUFA n3) ameliorate inflammation in different diseases and potentially improve neurological function after neuronal injury. Following spinal cord injury (SCI), inflammatory events result in caspase-1 mediated activation of interleukin-1 beta (IL-1b) and 18. We aim to evaluate the neuroprotective potency of PUFA n3 in suppressing the formation and activation of inflammasomes following SCI. Male Wistar rats were divided into four groups: control, SCI, SCI+PUFA n3, and SCI+Lipofundin MCT (medium-chain triglyceride; vehicle). PUFA n3 or vehicle was intravenously administered immediately after SCI and every 24 h for the next three days. We analyzed the expression of NLRP3, NLRP1, ASC, caspase-1, IL-1b, and 18 in the spinal cord. The distribution of microglia, oligodendrocytes, and astrocytes was assessed by immunohistochemistry analysis. Behavioral testing showed significantly improved locomotor recovery in PUFA n3-treated animals and the SCI-induced upregulation of inflammasome components was reduced. Histopathological evaluation confirmed the suppression of microgliosis, increased numbers of oligodendrocytes, and the prevention of demyelination by PUFA n3. Our data support the neuroprotective role of PUFA n3 by targeting the NLRP3 inflammasome. These findings provide evidence that PUFA n3 has therapeutic effects which potentially attenuate neuronal damage in SCI and possibly also in other neuronal injuries.
format	article
author	Maryam Baazm Victoria Behrens Cordian Beyer Omid Nikoubashman Adib Zendedel
author_facet	Maryam Baazm Victoria Behrens Cordian Beyer Omid Nikoubashman Adib Zendedel
author_sort	Maryam Baazm
title	Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model
title_short	Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model
title_full	Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model
title_fullStr	Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model
title_full_unstemmed	Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model
title_sort	regulation of inflammasomes by application of omega-3 polyunsaturated fatty acids in a spinal cord injury model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b0a25addda534cffa7ec58a326d4aa03
work_keys_str_mv	AT maryambaazm regulationofinflammasomesbyapplicationofomega3polyunsaturatedfattyacidsinaspinalcordinjurymodel AT victoriabehrens regulationofinflammasomesbyapplicationofomega3polyunsaturatedfattyacidsinaspinalcordinjurymodel AT cordianbeyer regulationofinflammasomesbyapplicationofomega3polyunsaturatedfattyacidsinaspinalcordinjurymodel AT omidnikoubashman regulationofinflammasomesbyapplicationofomega3polyunsaturatedfattyacidsinaspinalcordinjurymodel AT adibzendedel regulationofinflammasomesbyapplicationofomega3polyunsaturatedfattyacidsinaspinalcordinjurymodel
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Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model

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