BigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress

Abstract To cope with toxic levels of H2S, the plant pathogens Xylella fastidiosa and Agrobacterium tumefaciens employ the bigR operon to oxidize H2S into sulfite. The bigR operon is regulated by the transcriptional repressor BigR and it encodes a bifunctional sulfur transferase (ST) and sulfur diox...

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Autores principales:	Nayara Patricia Vieira de Lira, Bianca Alves Pauletti, Ana Carolina Marques, Carlos Alberto Perez, Raquel Caserta, Alessandra Alves de Souza, Aníbal Eugênio Vercesi, Adriana Franco Paes Leme, Celso Eduardo Benedetti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0a56edd1d12406d99e801a4e3706942
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spelling	oai:doaj.org-article:b0a56edd1d12406d99e801a4e37069422021-12-02T16:07:52ZBigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress10.1038/s41598-018-21974-x2045-2322https://doaj.org/article/b0a56edd1d12406d99e801a4e37069422018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21974-xhttps://doaj.org/toc/2045-2322Abstract To cope with toxic levels of H2S, the plant pathogens Xylella fastidiosa and Agrobacterium tumefaciens employ the bigR operon to oxidize H2S into sulfite. The bigR operon is regulated by the transcriptional repressor BigR and it encodes a bifunctional sulfur transferase (ST) and sulfur dioxygenase (SDO) enzyme, Blh, required for H2S oxidation and bacterial growth under hypoxia. However, how Blh operates to enhance bacterial survival under hypoxia and how BigR is deactivated to derepress operon transcription is unknown. Here, we show that the ST and SDO activities of Blh are in vitro coupled and necessary to oxidize sulfide into sulfite, and that Blh is critical to maintain the oxygen flux during A. tumefaciens respiration when oxygen becomes limited to cells. We also show that H2S and polysulfides inactivate BigR leading to operon transcription. Moreover, we show that sulfite, which is produced by Blh in the ST and SDO reactions, is toxic to Citrus sinensis and that X. fastidiosa-infected plants accumulate sulfite and higher transcript levels of sulfite detoxification enzymes, suggesting that they are under sulfite stress. These results indicate that BigR acts as a sulfide sensor in the H2S oxidation mechanism that allows pathogens to colonize plant tissues where oxygen is a limiting factor.Nayara Patricia Vieira de LiraBianca Alves PaulettiAna Carolina MarquesCarlos Alberto PerezRaquel CasertaAlessandra Alves de SouzaAníbal Eugênio VercesiAdriana Franco Paes LemeCelso Eduardo BenedettiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nayara Patricia Vieira de Lira Bianca Alves Pauletti Ana Carolina Marques Carlos Alberto Perez Raquel Caserta Alessandra Alves de Souza Aníbal Eugênio Vercesi Adriana Franco Paes Leme Celso Eduardo Benedetti BigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress
description	Abstract To cope with toxic levels of H2S, the plant pathogens Xylella fastidiosa and Agrobacterium tumefaciens employ the bigR operon to oxidize H2S into sulfite. The bigR operon is regulated by the transcriptional repressor BigR and it encodes a bifunctional sulfur transferase (ST) and sulfur dioxygenase (SDO) enzyme, Blh, required for H2S oxidation and bacterial growth under hypoxia. However, how Blh operates to enhance bacterial survival under hypoxia and how BigR is deactivated to derepress operon transcription is unknown. Here, we show that the ST and SDO activities of Blh are in vitro coupled and necessary to oxidize sulfide into sulfite, and that Blh is critical to maintain the oxygen flux during A. tumefaciens respiration when oxygen becomes limited to cells. We also show that H2S and polysulfides inactivate BigR leading to operon transcription. Moreover, we show that sulfite, which is produced by Blh in the ST and SDO reactions, is toxic to Citrus sinensis and that X. fastidiosa-infected plants accumulate sulfite and higher transcript levels of sulfite detoxification enzymes, suggesting that they are under sulfite stress. These results indicate that BigR acts as a sulfide sensor in the H2S oxidation mechanism that allows pathogens to colonize plant tissues where oxygen is a limiting factor.
format	article
author	Nayara Patricia Vieira de Lira Bianca Alves Pauletti Ana Carolina Marques Carlos Alberto Perez Raquel Caserta Alessandra Alves de Souza Aníbal Eugênio Vercesi Adriana Franco Paes Leme Celso Eduardo Benedetti
author_facet	Nayara Patricia Vieira de Lira Bianca Alves Pauletti Ana Carolina Marques Carlos Alberto Perez Raquel Caserta Alessandra Alves de Souza Aníbal Eugênio Vercesi Adriana Franco Paes Leme Celso Eduardo Benedetti
author_sort	Nayara Patricia Vieira de Lira
title	BigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress
title_short	BigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress
title_full	BigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress
title_fullStr	BigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress
title_full_unstemmed	BigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress
title_sort	bigr is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b0a56edd1d12406d99e801a4e3706942
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BigR is a sulfide sensor that regulates a sulfur transferase/dioxygenase required for aerobic respiration of plant bacteria under sulfide stress

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