MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS

Abstract Background MicroRNA-146a-5p (miR-146a-5p) is a key regulator of inflammatory processes. Expression of miR-146a-5p is altered in target organs of diabetic complications and deficiency of miR-146a-5p has been implicated in their pathogenesis. We investigated if serum miR-146a-5p levels were i...

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Autores principales:	Federica Barutta, Beatrice Corbetta, Stefania Bellini, Simonetta Guarrera, Giuseppe Matullo, Michela Scandella, Casper Schalkwijk, Coen D. Stehouwer, Nish Chaturvedi, Sabita S. Soedamah-Muthu, Marilena Durazzo, Gabriella Gruden
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	MicroRNAs Type 1 diabetes Retinopathy Cardiovascular diseases Medicine R
Acceso en línea:	https://doaj.org/article/b0a7a980d04c4a2db4937bdfa7a3c455
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spelling	oai:doaj.org-article:b0a7a980d04c4a2db4937bdfa7a3c4552021-11-28T12:06:48ZMicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS10.1186/s12967-021-03142-41479-5876https://doaj.org/article/b0a7a980d04c4a2db4937bdfa7a3c4552021-11-01T00:00:00Zhttps://doi.org/10.1186/s12967-021-03142-4https://doaj.org/toc/1479-5876Abstract Background MicroRNA-146a-5p (miR-146a-5p) is a key regulator of inflammatory processes. Expression of miR-146a-5p is altered in target organs of diabetic complications and deficiency of miR-146a-5p has been implicated in their pathogenesis. We investigated if serum miR-146a-5p levels were independently associated with micro/macrovascular complications of type 1 diabetes (DM1). Methods A nested case–control study from the EURODIAB PCS of 447 DM1 patients was performed. Cases (n = 294) had one or more complications of diabetes, whereas controls (n = 153) did not have any complication. Total RNA was isolated from all subjects and miR-146a-5p levels measured by qPCR. Both the endogenous controls U6 snRNA and the spike (Cel-miR-39) were used to normalize the results. Logistic regression analysis was carried out to investigate the association of miR-146a-5p with diabetes complications. Results MiR-146a-5p levels were significantly lower in cases [1.15 (0.32–3.34)] compared to controls [1.74 (0.44–6.74) P = 0.039]. Logistic regression analysis showed that levels of miR-146a-5p in the upper quartile were inversely associated with reduced odds ratio (OR) of all complications (OR 0.34 [95% CI 0.14–0.76]) and particularly with cardiovascular diseases (CVD) (OR 0.31 [95% CI 0.11–0.84]) and diabetic retinopathy (OR 0.40 [95% CI 0.16–0.99]), independently of age, sex, diabetes duration, A1c, hypertension, AER, eGFR, NT-proBNP, and TNF-α. Conclusions In this large cohort of DM1 patients, we reported an inverse and independent association of miR-146a-5p with diabetes chronic complications and in particular with CVD and retinopathy, suggesting that miR-146a-5p may be a novel candidate biomarker of DM1 complications.Federica BaruttaBeatrice CorbettaStefania BelliniSimonetta GuarreraGiuseppe MatulloMichela ScandellaCasper SchalkwijkCoen D. StehouwerNish ChaturvediSabita S. Soedamah-MuthuMarilena DurazzoGabriella GrudenBMCarticleMicroRNAsType 1 diabetesRetinopathyCardiovascular diseasesMedicineRENJournal of Translational Medicine, Vol 19, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MicroRNAs Type 1 diabetes Retinopathy Cardiovascular diseases Medicine R
spellingShingle	MicroRNAs Type 1 diabetes Retinopathy Cardiovascular diseases Medicine R Federica Barutta Beatrice Corbetta Stefania Bellini Simonetta Guarrera Giuseppe Matullo Michela Scandella Casper Schalkwijk Coen D. Stehouwer Nish Chaturvedi Sabita S. Soedamah-Muthu Marilena Durazzo Gabriella Gruden MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS
description	Abstract Background MicroRNA-146a-5p (miR-146a-5p) is a key regulator of inflammatory processes. Expression of miR-146a-5p is altered in target organs of diabetic complications and deficiency of miR-146a-5p has been implicated in their pathogenesis. We investigated if serum miR-146a-5p levels were independently associated with micro/macrovascular complications of type 1 diabetes (DM1). Methods A nested case–control study from the EURODIAB PCS of 447 DM1 patients was performed. Cases (n = 294) had one or more complications of diabetes, whereas controls (n = 153) did not have any complication. Total RNA was isolated from all subjects and miR-146a-5p levels measured by qPCR. Both the endogenous controls U6 snRNA and the spike (Cel-miR-39) were used to normalize the results. Logistic regression analysis was carried out to investigate the association of miR-146a-5p with diabetes complications. Results MiR-146a-5p levels were significantly lower in cases [1.15 (0.32–3.34)] compared to controls [1.74 (0.44–6.74) P = 0.039]. Logistic regression analysis showed that levels of miR-146a-5p in the upper quartile were inversely associated with reduced odds ratio (OR) of all complications (OR 0.34 [95% CI 0.14–0.76]) and particularly with cardiovascular diseases (CVD) (OR 0.31 [95% CI 0.11–0.84]) and diabetic retinopathy (OR 0.40 [95% CI 0.16–0.99]), independently of age, sex, diabetes duration, A1c, hypertension, AER, eGFR, NT-proBNP, and TNF-α. Conclusions In this large cohort of DM1 patients, we reported an inverse and independent association of miR-146a-5p with diabetes chronic complications and in particular with CVD and retinopathy, suggesting that miR-146a-5p may be a novel candidate biomarker of DM1 complications.
format	article
author	Federica Barutta Beatrice Corbetta Stefania Bellini Simonetta Guarrera Giuseppe Matullo Michela Scandella Casper Schalkwijk Coen D. Stehouwer Nish Chaturvedi Sabita S. Soedamah-Muthu Marilena Durazzo Gabriella Gruden
author_facet	Federica Barutta Beatrice Corbetta Stefania Bellini Simonetta Guarrera Giuseppe Matullo Michela Scandella Casper Schalkwijk Coen D. Stehouwer Nish Chaturvedi Sabita S. Soedamah-Muthu Marilena Durazzo Gabriella Gruden
author_sort	Federica Barutta
title	MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS
title_short	MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS
title_full	MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS
title_fullStr	MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS
title_full_unstemmed	MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS
title_sort	microrna 146a is associated with diabetic complications in type 1 diabetic patients from the eurodiab pcs
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b0a7a980d04c4a2db4937bdfa7a3c455
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MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS

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