CRISPR/Cas-based Diagnostics and Gene Therapy

Clustered regularly interspaced short palindromic repeats (CRISPR) technology, an easy, rapid, cost-effective, and precise gene-editing technique, has revolutionized diagnostics and gene therapy. Fast and accurate diagnosis of diseases is essential for point-of-care-testing (POCT) and specialized me...

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Autores principales:	Meiyu Qiu, Pei Li
Formato:	article
Lenguaje:	EN
Publicado:	Compuscript Ltd 2021
Materias:	clinical trails crispr/cas diagnostics gene therapy point-of-care-testing Medicine R
Acceso en línea:	https://doaj.org/article/b0aa443351d542798090fad645ccac65
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spelling	oai:doaj.org-article:b0aa443351d542798090fad645ccac652021-11-09T08:31:07ZCRISPR/Cas-based Diagnostics and Gene Therapy2712-008210.15212/bioi-2020-0048https://doaj.org/article/b0aa443351d542798090fad645ccac652021-11-01T00:00:00Zhttps://www.ingentaconnect.com/contentone/cscript/bioi/2021/00000002/00000003/art00004https://doaj.org/toc/2712-0082Clustered regularly interspaced short palindromic repeats (CRISPR) technology, an easy, rapid, cost-effective, and precise gene-editing technique, has revolutionized diagnostics and gene therapy. Fast and accurate diagnosis of diseases is essential for point-of-care-testing (POCT) and specialized medical institutes. The CRISPR-associated (Cas) proteins system shed light on the new diagnostics methods at point-of-care (POC) owning to its advantages. In addition, CRISPR/Cas-based gene-editing technology has led to various breakthroughs in gene therapy. It has been employed in clinical trials for a variety of untreatable diseases, including cancer, blood disorders, and other syndromes. Currently, the clinical application of CRISPR/Cas has been mainly focused on ex vivo therapies. Recently, tremendous efforts have been made in the development of ex vivo gene therapy based on CRISPR-Cas9. Despite these efforts, in vivo CRISPR/Cas gene therapy is only in its initial stage. Here, we review the milestones of CRISPR/Cas technologies that advanced the field of diagnostics and gene therapy. We also highlight the recent advances of diagnostics and gene therapy based on CRISPR/Cas technology. In the last section, we discuss the strength and significant challenges of the CRISPR/Cas technology for its future clinical usage in diagnosis and gene therapy.Meiyu QiuPei LiCompuscript Ltdarticleclinical trailscrispr/casdiagnosticsgene therapypoint-of-care-testingMedicineRENBIO Integration, Vol 2, Iss 3, Pp 121-129 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	clinical trails crispr/cas diagnostics gene therapy point-of-care-testing Medicine R
spellingShingle	clinical trails crispr/cas diagnostics gene therapy point-of-care-testing Medicine R Meiyu Qiu Pei Li CRISPR/Cas-based Diagnostics and Gene Therapy
description	Clustered regularly interspaced short palindromic repeats (CRISPR) technology, an easy, rapid, cost-effective, and precise gene-editing technique, has revolutionized diagnostics and gene therapy. Fast and accurate diagnosis of diseases is essential for point-of-care-testing (POCT) and specialized medical institutes. The CRISPR-associated (Cas) proteins system shed light on the new diagnostics methods at point-of-care (POC) owning to its advantages. In addition, CRISPR/Cas-based gene-editing technology has led to various breakthroughs in gene therapy. It has been employed in clinical trials for a variety of untreatable diseases, including cancer, blood disorders, and other syndromes. Currently, the clinical application of CRISPR/Cas has been mainly focused on ex vivo therapies. Recently, tremendous efforts have been made in the development of ex vivo gene therapy based on CRISPR-Cas9. Despite these efforts, in vivo CRISPR/Cas gene therapy is only in its initial stage. Here, we review the milestones of CRISPR/Cas technologies that advanced the field of diagnostics and gene therapy. We also highlight the recent advances of diagnostics and gene therapy based on CRISPR/Cas technology. In the last section, we discuss the strength and significant challenges of the CRISPR/Cas technology for its future clinical usage in diagnosis and gene therapy.
format	article
author	Meiyu Qiu Pei Li
author_facet	Meiyu Qiu Pei Li
author_sort	Meiyu Qiu
title	CRISPR/Cas-based Diagnostics and Gene Therapy
title_short	CRISPR/Cas-based Diagnostics and Gene Therapy
title_full	CRISPR/Cas-based Diagnostics and Gene Therapy
title_fullStr	CRISPR/Cas-based Diagnostics and Gene Therapy
title_full_unstemmed	CRISPR/Cas-based Diagnostics and Gene Therapy
title_sort	crispr/cas-based diagnostics and gene therapy
publisher	Compuscript Ltd
publishDate	2021
url	https://doaj.org/article/b0aa443351d542798090fad645ccac65
work_keys_str_mv	AT meiyuqiu crisprcasbaseddiagnosticsandgenetherapy AT peili crisprcasbaseddiagnosticsandgenetherapy
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CRISPR/Cas-based Diagnostics and Gene Therapy

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