Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements

Affinity purification-mass spectrometry (AP-MS) can identify endogenous protein interactions but the need for high amounts of input material still limits its applicability. Here, the authors present a microfluidic-based AP-MS workflow that can capture protein interactions from 50─100-fold less input...

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Autores principales:	Cristina Furlan, René A. M. Dirks, Peter C. Thomas, Robert C. Jones, Jing Wang, Mark Lynch, Hendrik Marks, Michiel Vermeulen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b0ab23fc21264551ba1dea982ce9a0d1
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spelling	oai:doaj.org-article:b0ab23fc21264551ba1dea982ce9a0d12021-12-02T14:40:20ZMiniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements10.1038/s41467-019-09533-y2041-1723https://doaj.org/article/b0ab23fc21264551ba1dea982ce9a0d12019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09533-yhttps://doaj.org/toc/2041-1723Affinity purification-mass spectrometry (AP-MS) can identify endogenous protein interactions but the need for high amounts of input material still limits its applicability. Here, the authors present a microfluidic-based AP-MS workflow that can capture protein interactions from 50─100-fold less input material than conventional approaches.Cristina FurlanRené A. M. DirksPeter C. ThomasRobert C. JonesJing WangMark LynchHendrik MarksMichiel VermeulenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Cristina Furlan René A. M. Dirks Peter C. Thomas Robert C. Jones Jing Wang Mark Lynch Hendrik Marks Michiel Vermeulen Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
description	Affinity purification-mass spectrometry (AP-MS) can identify endogenous protein interactions but the need for high amounts of input material still limits its applicability. Here, the authors present a microfluidic-based AP-MS workflow that can capture protein interactions from 50─100-fold less input material than conventional approaches.
format	article
author	Cristina Furlan René A. M. Dirks Peter C. Thomas Robert C. Jones Jing Wang Mark Lynch Hendrik Marks Michiel Vermeulen
author_facet	Cristina Furlan René A. M. Dirks Peter C. Thomas Robert C. Jones Jing Wang Mark Lynch Hendrik Marks Michiel Vermeulen
author_sort	Cristina Furlan
title	Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
title_short	Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
title_full	Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
title_fullStr	Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
title_full_unstemmed	Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
title_sort	miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/b0ab23fc21264551ba1dea982ce9a0d1
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Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements

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