Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections

Abstract Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainab...

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Autores principales:	Joar E. Pinto Torres, Julie Goossens, Jianzu Ding, Zeng Li, Shaohong Lu, Didier Vertommen, Peter Naniima, Rui Chen, Serge Muyldermans, Yann G.-J. Sterckx, Stefan Magez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0ab489491d949b589362f495b917920
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spelling	oai:doaj.org-article:b0ab489491d949b589362f495b9179202021-12-02T15:07:56ZDevelopment of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections10.1038/s41598-018-26732-72045-2322https://doaj.org/article/b0ab489491d949b589362f495b9179202018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26732-7https://doaj.org/toc/2045-2322Abstract Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainable livestock rearing very strenuous. Active case-finding and the identification of infected animals prior to initiation of drug treatment requires the availability of sensitive and specific diagnostic tests. In this paper, we describe the development of two heterologous sandwich assay formats (ELISA and LFA) for T. congolense detection through the use of Nanobodies (Nbs). The immunisation of an alpaca with a secretome mix from two T. congolense strains resulted in the identification of a Nb pair (Nb44/Nb42) that specifically targets the glycolytic enzyme pyruvate kinase. We demonstrate that the Nb44/Nb42 ELISA and LFA can be employed to detect parasitaemia in plasma samples from experimentally infected mice and cattle and, additionally, that they can serve as ‘test-of-cure’ tools. Altogether, the findings in this paper present the development and evaluation of the first Nb-based antigen detection LFA to identify active T. congolense infections.Joar E. Pinto TorresJulie GoossensJianzu DingZeng LiShaohong LuDidier VertommenPeter NaniimaRui ChenSerge MuyldermansYann G.-J. SterckxStefan MagezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joar E. Pinto Torres Julie Goossens Jianzu Ding Zeng Li Shaohong Lu Didier Vertommen Peter Naniima Rui Chen Serge Muyldermans Yann G.-J. Sterckx Stefan Magez Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections
description	Abstract Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainable livestock rearing very strenuous. Active case-finding and the identification of infected animals prior to initiation of drug treatment requires the availability of sensitive and specific diagnostic tests. In this paper, we describe the development of two heterologous sandwich assay formats (ELISA and LFA) for T. congolense detection through the use of Nanobodies (Nbs). The immunisation of an alpaca with a secretome mix from two T. congolense strains resulted in the identification of a Nb pair (Nb44/Nb42) that specifically targets the glycolytic enzyme pyruvate kinase. We demonstrate that the Nb44/Nb42 ELISA and LFA can be employed to detect parasitaemia in plasma samples from experimentally infected mice and cattle and, additionally, that they can serve as ‘test-of-cure’ tools. Altogether, the findings in this paper present the development and evaluation of the first Nb-based antigen detection LFA to identify active T. congolense infections.
format	article
author	Joar E. Pinto Torres Julie Goossens Jianzu Ding Zeng Li Shaohong Lu Didier Vertommen Peter Naniima Rui Chen Serge Muyldermans Yann G.-J. Sterckx Stefan Magez
author_facet	Joar E. Pinto Torres Julie Goossens Jianzu Ding Zeng Li Shaohong Lu Didier Vertommen Peter Naniima Rui Chen Serge Muyldermans Yann G.-J. Sterckx Stefan Magez
author_sort	Joar E. Pinto Torres
title	Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections
title_short	Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections
title_full	Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections
title_fullStr	Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections
title_full_unstemmed	Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections
title_sort	development of a nanobody-based lateral flow assay to detect active trypanosoma congolense infections
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b0ab489491d949b589362f495b917920
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Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections

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