Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice

Abstract Immunotherapy is a novel treatment approach for cancers; however, its therapeutic effects are impeded by myeloid-derived suppressor cells (MDSCs). This study aimed to determine how MDSCs are expanded in cancer hosts. MDSCs were positive for Gr-1 and CD11b. Hepa1-6 hepatoma cells, EL4 lympho...

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Autores principales:	Wei-Chen Lee, Pao-Yueh Hsu, Hsiu-Ying Hsu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0ad083767e84fdfa68c5b579fa74439
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spelling	oai:doaj.org-article:b0ad083767e84fdfa68c5b579fa744392021-12-02T16:24:49ZStem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice10.1038/s41598-020-68061-82045-2322https://doaj.org/article/b0ad083767e84fdfa68c5b579fa744392020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68061-8https://doaj.org/toc/2045-2322Abstract Immunotherapy is a novel treatment approach for cancers; however, its therapeutic effects are impeded by myeloid-derived suppressor cells (MDSCs). This study aimed to determine how MDSCs are expanded in cancer hosts. MDSCs were positive for Gr-1 and CD11b. Hepa1-6 hepatoma cells, EL4 lymphoma cells, and mice bearing Hepa1-6 hepatoma or lymphoma were examined. Following the inoculation of Hepa1-6 cells into the flanks of mice, a linear correlation was evident between the frequency of MDSCs in the spleen and tumor sizes. MDSC numbers diminished gradually and returned to the normal level within 3 weeks if the tumors were excised. To identify the cytokines produced by tumor cells that allowed expansion of MDSCs, cytokines in Hepa1-6 cell culture medium and murine serum were examined using a cytokine array. Stem cell factor (SCF) was implicated as the relevant cytokine. When recombinant SCF was added to the spleen cell culture medium, MDSC expansion could occur. In the presence of c-kit blockade, this effect of SCF was partially reversed. In conclusion, MDSCs can be expanded in tumor cells in a process that involves SCF released by tumor cells.Wei-Chen LeePao-Yueh HsuHsiu-Ying HsuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei-Chen Lee Pao-Yueh Hsu Hsiu-Ying Hsu Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice
description	Abstract Immunotherapy is a novel treatment approach for cancers; however, its therapeutic effects are impeded by myeloid-derived suppressor cells (MDSCs). This study aimed to determine how MDSCs are expanded in cancer hosts. MDSCs were positive for Gr-1 and CD11b. Hepa1-6 hepatoma cells, EL4 lymphoma cells, and mice bearing Hepa1-6 hepatoma or lymphoma were examined. Following the inoculation of Hepa1-6 cells into the flanks of mice, a linear correlation was evident between the frequency of MDSCs in the spleen and tumor sizes. MDSC numbers diminished gradually and returned to the normal level within 3 weeks if the tumors were excised. To identify the cytokines produced by tumor cells that allowed expansion of MDSCs, cytokines in Hepa1-6 cell culture medium and murine serum were examined using a cytokine array. Stem cell factor (SCF) was implicated as the relevant cytokine. When recombinant SCF was added to the spleen cell culture medium, MDSC expansion could occur. In the presence of c-kit blockade, this effect of SCF was partially reversed. In conclusion, MDSCs can be expanded in tumor cells in a process that involves SCF released by tumor cells.
format	article
author	Wei-Chen Lee Pao-Yueh Hsu Hsiu-Ying Hsu
author_facet	Wei-Chen Lee Pao-Yueh Hsu Hsiu-Ying Hsu
author_sort	Wei-Chen Lee
title	Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice
title_short	Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice
title_full	Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice
title_fullStr	Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice
title_full_unstemmed	Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice
title_sort	stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b0ad083767e84fdfa68c5b579fa74439
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Stem cell factor produced by tumor cells expands myeloid-derived suppressor cells in mice

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