Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers

PMOs (phosphorodiamidate morpholino oligomers) have huge potential for antisense therapy but complex and slow synthesis limits application. Here, the authors report the development of automated flow synthesis methods which reduce nucleobase coupling times from hours to minutes removing human errors...

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Autores principales:	Chengxi Li, Alex J. Callahan, Mark D. Simon, Kyle A. Totaro, Alexander J. Mijalis, Kruttika-Suhas Phadke, Genwei Zhang, Nina Hartrampf, Carly K. Schissel, Ming Zhou, Hong Zong, Gunnar J. Hanson, Andrei Loas, Nicola L. B. Pohl, David E. Verhoeven, Bradley L. Pentelute
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b0ad40ceaf224d3c88e0a5f7d5670117
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spelling	oai:doaj.org-article:b0ad40ceaf224d3c88e0a5f7d56701172021-12-02T16:26:40ZFully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers10.1038/s41467-021-24598-42041-1723https://doaj.org/article/b0ad40ceaf224d3c88e0a5f7d56701172021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24598-4https://doaj.org/toc/2041-1723PMOs (phosphorodiamidate morpholino oligomers) have huge potential for antisense therapy but complex and slow synthesis limits application. Here, the authors report the development of automated flow synthesis methods which reduce nucleobase coupling times from hours to minutes removing human errors and allow for high-throughput production.Chengxi LiAlex J. CallahanMark D. SimonKyle A. TotaroAlexander J. MijalisKruttika-Suhas PhadkeGenwei ZhangNina HartrampfCarly K. SchisselMing ZhouHong ZongGunnar J. HansonAndrei LoasNicola L. B. PohlDavid E. VerhoevenBradley L. PenteluteNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Chengxi Li Alex J. Callahan Mark D. Simon Kyle A. Totaro Alexander J. Mijalis Kruttika-Suhas Phadke Genwei Zhang Nina Hartrampf Carly K. Schissel Ming Zhou Hong Zong Gunnar J. Hanson Andrei Loas Nicola L. B. Pohl David E. Verhoeven Bradley L. Pentelute Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
description	PMOs (phosphorodiamidate morpholino oligomers) have huge potential for antisense therapy but complex and slow synthesis limits application. Here, the authors report the development of automated flow synthesis methods which reduce nucleobase coupling times from hours to minutes removing human errors and allow for high-throughput production.
format	article
author	Chengxi Li Alex J. Callahan Mark D. Simon Kyle A. Totaro Alexander J. Mijalis Kruttika-Suhas Phadke Genwei Zhang Nina Hartrampf Carly K. Schissel Ming Zhou Hong Zong Gunnar J. Hanson Andrei Loas Nicola L. B. Pohl David E. Verhoeven Bradley L. Pentelute
author_facet	Chengxi Li Alex J. Callahan Mark D. Simon Kyle A. Totaro Alexander J. Mijalis Kruttika-Suhas Phadke Genwei Zhang Nina Hartrampf Carly K. Schissel Ming Zhou Hong Zong Gunnar J. Hanson Andrei Loas Nicola L. B. Pohl David E. Verhoeven Bradley L. Pentelute
author_sort	Chengxi Li
title	Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
title_short	Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
title_full	Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
title_fullStr	Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
title_full_unstemmed	Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
title_sort	fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0ad40ceaf224d3c88e0a5f7d5670117
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Fully automated fast-flow synthesis of antisense phosphorodiamidate morpholino oligomers

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