Deep sequencing of RNA from ancient maize kernels.

The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypot...

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Autores principales:	Sarah L Fordyce, Maria C Ávila-Arcos, Morten Rasmussen, Enrico Cappellini, J Alberto Romero-Navarro, Nathan Wales, David E Alquezar-Planas, Steven Penfield, Terence A Brown, Jean-Philippe Vielle-Calzada, Rafael Montiel, Tina Jørgensen, Nancy Odegaard, Michael Jacobs, Bernardo Arriaza, Thomas F G Higham, Christopher Bronk Ramsey, Eske Willerslev, M Thomas P Gilbert
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0b48a9c0776467e9bac0e115d298efc
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spelling	oai:doaj.org-article:b0b48a9c0776467e9bac0e115d298efc2021-11-18T08:01:55ZDeep sequencing of RNA from ancient maize kernels.1932-620310.1371/journal.pone.0050961https://doaj.org/article/b0b48a9c0776467e9bac0e115d298efc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23326310/?tool=EBIhttps://doaj.org/toc/1932-6203The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.Sarah L FordyceMaria C Ávila-ArcosMorten RasmussenEnrico CappelliniJ Alberto Romero-NavarroNathan WalesDavid E Alquezar-PlanasSteven PenfieldTerence A BrownJean-Philippe Vielle-CalzadaRafael MontielTina JørgensenNancy OdegaardMichael JacobsBernardo ArriazaThomas F G HighamChristopher Bronk RamseyEske WillerslevM Thomas P GilbertPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e50961 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah L Fordyce Maria C Ávila-Arcos Morten Rasmussen Enrico Cappellini J Alberto Romero-Navarro Nathan Wales David E Alquezar-Planas Steven Penfield Terence A Brown Jean-Philippe Vielle-Calzada Rafael Montiel Tina Jørgensen Nancy Odegaard Michael Jacobs Bernardo Arriaza Thomas F G Higham Christopher Bronk Ramsey Eske Willerslev M Thomas P Gilbert Deep sequencing of RNA from ancient maize kernels.
description	The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.
format	article
author	Sarah L Fordyce Maria C Ávila-Arcos Morten Rasmussen Enrico Cappellini J Alberto Romero-Navarro Nathan Wales David E Alquezar-Planas Steven Penfield Terence A Brown Jean-Philippe Vielle-Calzada Rafael Montiel Tina Jørgensen Nancy Odegaard Michael Jacobs Bernardo Arriaza Thomas F G Higham Christopher Bronk Ramsey Eske Willerslev M Thomas P Gilbert
author_facet	Sarah L Fordyce Maria C Ávila-Arcos Morten Rasmussen Enrico Cappellini J Alberto Romero-Navarro Nathan Wales David E Alquezar-Planas Steven Penfield Terence A Brown Jean-Philippe Vielle-Calzada Rafael Montiel Tina Jørgensen Nancy Odegaard Michael Jacobs Bernardo Arriaza Thomas F G Higham Christopher Bronk Ramsey Eske Willerslev M Thomas P Gilbert
author_sort	Sarah L Fordyce
title	Deep sequencing of RNA from ancient maize kernels.
title_short	Deep sequencing of RNA from ancient maize kernels.
title_full	Deep sequencing of RNA from ancient maize kernels.
title_fullStr	Deep sequencing of RNA from ancient maize kernels.
title_full_unstemmed	Deep sequencing of RNA from ancient maize kernels.
title_sort	deep sequencing of rna from ancient maize kernels.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b0b48a9c0776467e9bac0e115d298efc
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Deep sequencing of RNA from ancient maize kernels.

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