Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety

Abstract Cadmium (Cd) contamination of rice is a serious food safety issue that has recently been gaining significant public attention. Therefore, reduction of Cd accumulation in rice grains is an important objective of rice breeding. The use of favourable alleles of Cd accumulating genes using mark...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kai Wang, Tian-ze Yan, Shi-long Xu, Xu Yan, Qun-feng Zhou, Xin-hui Zhao, Yan-feng Li, Zhong-xiu Wu, Peng Qin, Chen-jian Fu, Jun Fu, Yan-biao Zhou, Yuan-zhu Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0bdbe5fbc6a4fc184de697847e4d58c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b0bdbe5fbc6a4fc184de697847e4d58c
record_format	dspace
spelling	oai:doaj.org-article:b0bdbe5fbc6a4fc184de697847e4d58c2021-12-02T17:04:59ZValidating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety10.1038/s41598-021-85324-02045-2322https://doaj.org/article/b0bdbe5fbc6a4fc184de697847e4d58c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85324-0https://doaj.org/toc/2045-2322Abstract Cadmium (Cd) contamination of rice is a serious food safety issue that has recently been gaining significant public attention. Therefore, reduction of Cd accumulation in rice grains is an important objective of rice breeding. The use of favourable alleles of Cd accumulating genes using marker-assisted selection (MAS) is theoretically feasible. In this study, we validated a segment covering OsHMA3-OsNramp5-OsNramp1 on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety. The OsHMA3-OsNramp5-OsNramp1 jap haplotype significantly decreased grain Cd concentration in middle-season indica genetic background. The improved 9311 carrying the OsHMA3-OsNramp5-OsNramp1 jap haplotype with recurrent parent genome recovery of up to 91.6% resulted in approximately 31.8% decrease in Cd accumulation in the grain and with no penalty on yield. There is a genetic linkage-drag between OsHMA3-OsNramp5-OsNramp1 jap and the gene conditioning heading to days (HTD) in the early-season indica genetic background. Because the OsHMA3-OsNramp5-OsNramp1-Ghd7 jap haplotype significantly increases grain Cd concentration and prolongs growth duration, the linkage-drag between OsHMA3-OsNramp5-OsNramp1 and Ghd7 should be broken down by large segregating populations or gene editing. A novel allele of OsHMA3 was identified from a wide-compatibility japonica cultivar, the expression differences of OsNramp1 and OsNramp5 in roots might contribute the Cd accumulating variation between japonica and indica variety.Kai WangTian-ze YanShi-long XuXu YanQun-feng ZhouXin-hui ZhaoYan-feng LiZhong-xiu WuPeng QinChen-jian FuJun FuYan-biao ZhouYuan-zhu YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Wang Tian-ze Yan Shi-long Xu Xu Yan Qun-feng Zhou Xin-hui Zhao Yan-feng Li Zhong-xiu Wu Peng Qin Chen-jian Fu Jun Fu Yan-biao Zhou Yuan-zhu Yang Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
description	Abstract Cadmium (Cd) contamination of rice is a serious food safety issue that has recently been gaining significant public attention. Therefore, reduction of Cd accumulation in rice grains is an important objective of rice breeding. The use of favourable alleles of Cd accumulating genes using marker-assisted selection (MAS) is theoretically feasible. In this study, we validated a segment covering OsHMA3-OsNramp5-OsNramp1 on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety. The OsHMA3-OsNramp5-OsNramp1 jap haplotype significantly decreased grain Cd concentration in middle-season indica genetic background. The improved 9311 carrying the OsHMA3-OsNramp5-OsNramp1 jap haplotype with recurrent parent genome recovery of up to 91.6% resulted in approximately 31.8% decrease in Cd accumulation in the grain and with no penalty on yield. There is a genetic linkage-drag between OsHMA3-OsNramp5-OsNramp1 jap and the gene conditioning heading to days (HTD) in the early-season indica genetic background. Because the OsHMA3-OsNramp5-OsNramp1-Ghd7 jap haplotype significantly increases grain Cd concentration and prolongs growth duration, the linkage-drag between OsHMA3-OsNramp5-OsNramp1 and Ghd7 should be broken down by large segregating populations or gene editing. A novel allele of OsHMA3 was identified from a wide-compatibility japonica cultivar, the expression differences of OsNramp1 and OsNramp5 in roots might contribute the Cd accumulating variation between japonica and indica variety.
format	article
author	Kai Wang Tian-ze Yan Shi-long Xu Xu Yan Qun-feng Zhou Xin-hui Zhao Yan-feng Li Zhong-xiu Wu Peng Qin Chen-jian Fu Jun Fu Yan-biao Zhou Yuan-zhu Yang
author_facet	Kai Wang Tian-ze Yan Shi-long Xu Xu Yan Qun-feng Zhou Xin-hui Zhao Yan-feng Li Zhong-xiu Wu Peng Qin Chen-jian Fu Jun Fu Yan-biao Zhou Yuan-zhu Yang
author_sort	Kai Wang
title	Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
title_short	Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
title_full	Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
title_fullStr	Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
title_full_unstemmed	Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
title_sort	validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0bdbe5fbc6a4fc184de697847e4d58c
work_keys_str_mv	AT kaiwang validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT tianzeyan validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT shilongxu validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT xuyan validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT qunfengzhou validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT xinhuizhao validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT yanfengli validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT zhongxiuwu validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT pengqin validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT chenjianfu validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT junfu validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT yanbiaozhou validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety AT yuanzhuyang validatingasegmentonchromosome7ofjaponicaforestablishinglowcadmiumaccumulatingindicaricevariety
_version_	1718381809161469952

Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety

Ejemplares similares