Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues
Abstract Background Single-cell RNA sequencing (scRNA-seq) provides new insights to address biological and medical questions, and it will benefit more from the ultralow input RNA or subcellular sequencing. Results Here, we present a highly sensitive library construction protocol for ultralow input R...
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Auteurs principaux: | Erteng Jia, Huajuan Shi, Ying Wang, Ying Zhou, Zhiyu Liu, Min Pan, Yunfei Bai, Xiangwei Zhao, Qinyu Ge |
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Format: | article |
Langue: | EN |
Publié: |
BMC
2021
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Accès en ligne: | https://doaj.org/article/b0be696efd3f4fac88cee77b65386bc1 |
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