Optimization of library preparation based on SMART for ultralow RNA-seq in mice brain tissues

Abstract Background Single-cell RNA sequencing (scRNA-seq) provides new insights to address biological and medical questions, and it will benefit more from the ultralow input RNA or subcellular sequencing. Results Here, we present a highly sensitive library construction protocol for ultralow input R...

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Autores principales: Erteng Jia, Huajuan Shi, Ying Wang, Ying Zhou, Zhiyu Liu, Min Pan, Yunfei Bai, Xiangwei Zhao, Qinyu Ge
Formato: article
Lenguaje:EN
Publicado: BMC 2021
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Acceso en línea:https://doaj.org/article/b0be696efd3f4fac88cee77b65386bc1
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