Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae

Klebsiella pneumoniae is a dominant cause of community-acquired and nosocomial infections, specifically among immunocompromised individuals. The increasing occurrence of multidrug-resistant (MDR) isolates has significantly impacted the effectiveness of antimicrobial agents. As antibiotic resistance...

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Autores principales:	Na Li, Yigang Zeng, Rong Bao, Tongyu Zhu, Demeng Tan, Bijie Hu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bacteriophage phage therapy Klebsiella pneumoniae phage-host interactions multidrug resistance Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b0c0e2a0b88f4b01ba132524a1507568
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spelling	oai:doaj.org-article:b0c0e2a0b88f4b01ba132524a15075682021-12-03T04:51:47ZIsolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae2235-298810.3389/fcimb.2021.792305https://doaj.org/article/b0c0e2a0b88f4b01ba132524a15075682021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.792305/fullhttps://doaj.org/toc/2235-2988Klebsiella pneumoniae is a dominant cause of community-acquired and nosocomial infections, specifically among immunocompromised individuals. The increasing occurrence of multidrug-resistant (MDR) isolates has significantly impacted the effectiveness of antimicrobial agents. As antibiotic resistance is becoming increasingly prevalent worldwide, the use of bacteriophages to treat pathogenic bacterial infections has recently gained attention. Elucidating the details of phage-bacteria interactions will provide insights into phage biology and the better development of phage therapy. In this study, a total of 22 K. pneumoniae isolates were assessed for their genetic and phenotypic relatedness by multi-locus sequence typing (MLST), endonuclease S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), and in vitro antibiotic susceptibility testing. In addition, the beta-lactamase gene (blaKPC) was characterized to determine the spread and outbreak of K. pneumoniae carbapenemase (KPC)-producing enterobacterial pathogens. Using these ST11 carbapenem-resistant K. pneumoniae isolates, three phages (NL_ZS_1, NL_ZS_2, and NL_ZS_3) from the family of Podoviridae were isolated and characterized to evaluate the application of lytic phages against the MDR K. pneumoniae isolates. In vitro inhibition assays with three phages and K. pneumoniae strain ZS15 demonstrated the strong lytic potential of the phages, however, followed by the rapid growth of phage-resistant and phage-sensitive mutants, suggesting several anti-phage mechanisms had developed in the host populations. Together, this data adds more comprehensive knowledge to known phage biology and further emphasizes their complexity and future challenges to overcome prior to using phages for controlling this important MDR bacterium.Na LiYigang ZengRong BaoTongyu ZhuDemeng TanBijie HuFrontiers Media S.A.articlebacteriophagephage therapyKlebsiella pneumoniaephage-host interactionsmultidrug resistanceMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriophage phage therapy Klebsiella pneumoniae phage-host interactions multidrug resistance Microbiology QR1-502
spellingShingle	bacteriophage phage therapy Klebsiella pneumoniae phage-host interactions multidrug resistance Microbiology QR1-502 Na Li Yigang Zeng Rong Bao Tongyu Zhu Demeng Tan Bijie Hu Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae
description	Klebsiella pneumoniae is a dominant cause of community-acquired and nosocomial infections, specifically among immunocompromised individuals. The increasing occurrence of multidrug-resistant (MDR) isolates has significantly impacted the effectiveness of antimicrobial agents. As antibiotic resistance is becoming increasingly prevalent worldwide, the use of bacteriophages to treat pathogenic bacterial infections has recently gained attention. Elucidating the details of phage-bacteria interactions will provide insights into phage biology and the better development of phage therapy. In this study, a total of 22 K. pneumoniae isolates were assessed for their genetic and phenotypic relatedness by multi-locus sequence typing (MLST), endonuclease S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), and in vitro antibiotic susceptibility testing. In addition, the beta-lactamase gene (blaKPC) was characterized to determine the spread and outbreak of K. pneumoniae carbapenemase (KPC)-producing enterobacterial pathogens. Using these ST11 carbapenem-resistant K. pneumoniae isolates, three phages (NL_ZS_1, NL_ZS_2, and NL_ZS_3) from the family of Podoviridae were isolated and characterized to evaluate the application of lytic phages against the MDR K. pneumoniae isolates. In vitro inhibition assays with three phages and K. pneumoniae strain ZS15 demonstrated the strong lytic potential of the phages, however, followed by the rapid growth of phage-resistant and phage-sensitive mutants, suggesting several anti-phage mechanisms had developed in the host populations. Together, this data adds more comprehensive knowledge to known phage biology and further emphasizes their complexity and future challenges to overcome prior to using phages for controlling this important MDR bacterium.
format	article
author	Na Li Yigang Zeng Rong Bao Tongyu Zhu Demeng Tan Bijie Hu
author_facet	Na Li Yigang Zeng Rong Bao Tongyu Zhu Demeng Tan Bijie Hu
author_sort	Na Li
title	Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae
title_short	Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae
title_full	Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae
title_fullStr	Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae
title_full_unstemmed	Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae
title_sort	isolation and characterization of novel phages targeting pathogenic klebsiella pneumoniae
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b0c0e2a0b88f4b01ba132524a1507568
work_keys_str_mv	AT nali isolationandcharacterizationofnovelphagestargetingpathogenicklebsiellapneumoniae AT yigangzeng isolationandcharacterizationofnovelphagestargetingpathogenicklebsiellapneumoniae AT rongbao isolationandcharacterizationofnovelphagestargetingpathogenicklebsiellapneumoniae AT tongyuzhu isolationandcharacterizationofnovelphagestargetingpathogenicklebsiellapneumoniae AT demengtan isolationandcharacterizationofnovelphagestargetingpathogenicklebsiellapneumoniae AT bijiehu isolationandcharacterizationofnovelphagestargetingpathogenicklebsiellapneumoniae
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Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae

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