APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES

Abstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) ar...

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Autores principales:	M. A. Plotnikova, S. A. Klotchenko, V. V. Egorov, K. P. Nikulenkov, A. V. Vasin, O. I. Kisselev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	cytokines antibodies microarray immunological analysis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b0c4e1a20bfc4d05bc7aa47d16423792
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spelling	oai:doaj.org-article:b0c4e1a20bfc4d05bc7aa47d164237922021-11-18T08:03:40ZAPPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES1563-06252313-741X10.15789/1563-0625-2012-4-5-415-418https://doaj.org/article/b0c4e1a20bfc4d05bc7aa47d164237922014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/378https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) are demonstrated with human IL-2, IL-4, IL-10, and TNF assays. This diagnostic technique may be further used both for research applications and in clinical trials.M. A. PlotnikovaS. A. KlotchenkoV. V. EgorovK. P. NikulenkovA. V. VasinO. I. KisselevSPb RAACIarticlecytokinesantibodiesmicroarrayimmunological analysisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 14, Iss 4-5, Pp 415-418 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	cytokines antibodies microarray immunological analysis Immunologic diseases. Allergy RC581-607
spellingShingle	cytokines antibodies microarray immunological analysis Immunologic diseases. Allergy RC581-607 M. A. Plotnikova S. A. Klotchenko V. V. Egorov K. P. Nikulenkov A. V. Vasin O. I. Kisselev APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
description	Abstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) are demonstrated with human IL-2, IL-4, IL-10, and TNF assays. This diagnostic technique may be further used both for research applications and in clinical trials.
format	article
author	M. A. Plotnikova S. A. Klotchenko V. V. Egorov K. P. Nikulenkov A. V. Vasin O. I. Kisselev
author_facet	M. A. Plotnikova S. A. Klotchenko V. V. Egorov K. P. Nikulenkov A. V. Vasin O. I. Kisselev
author_sort	M. A. Plotnikova
title	APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_short	APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_full	APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_fullStr	APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_full_unstemmed	APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_sort	application of solid-phase, enzyme-linked immunoassays in microarray formate for quantitative determination of cytokines
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/b0c4e1a20bfc4d05bc7aa47d16423792
work_keys_str_mv	AT maplotnikova applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines AT saklotchenko applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines AT vvegorov applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines AT kpnikulenkov applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines AT avvasin applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines AT oikisselev applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines
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APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES

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