Proteome regulation during Olea europaea fruit development.

<h4>Background</h4>Widespread in the Mediterranean basin, Olea europaea trees are gaining worldwide popularity for the nutritional and cancer-protective properties of the oil, mechanically extracted from ripe fruits. Fruit development is a physiological process with remarkable impact on...

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Autores principales:	Linda Bianco, Fiammetta Alagna, Luciana Baldoni, Christine Finnie, Birte Svensson, Gaetano Perrotta
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0d1613e988e4067813f2d33020c1a2e
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spelling	oai:doaj.org-article:b0d1613e988e4067813f2d33020c1a2e2021-11-18T08:01:06ZProteome regulation during Olea europaea fruit development.1932-620310.1371/journal.pone.0053563https://doaj.org/article/b0d1613e988e4067813f2d33020c1a2e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23349718/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Widespread in the Mediterranean basin, Olea europaea trees are gaining worldwide popularity for the nutritional and cancer-protective properties of the oil, mechanically extracted from ripe fruits. Fruit development is a physiological process with remarkable impact on the modulation of the biosynthesis of compounds affecting the quality of the drupes as well as the final composition of the olive oil. Proteomics offers the possibility to dig deeper into the major changes during fruit development, including the important phase of ripening, and to classify temporal patterns of protein accumulation occurring during these complex physiological processes.<h4>Methodology/principal findings</h4>In this work, we started monitoring the proteome variations associated with olive fruit development by using comparative proteomics coupled to mass spectrometry. Proteins extracted from drupes at three different developmental stages were separated on 2-DE and subjected to image analysis. 247 protein spots were revealed as differentially accumulated. Proteins were identified from a total of 121 spots and discussed in relation to olive drupe metabolic changes occurring during fruit development. In order to evaluate if changes observed at the protein level were consistent with changes of mRNAs, proteomic data produced in the present work were compared with transcriptomic data elaborated during previous studies.<h4>Conclusions/significance</h4>This study identifies a number of proteins responsible for quality traits of cv. Coratina, with particular regard to proteins associated to the metabolism of fatty acids, phenolic and aroma compounds. Proteins involved in fruit photosynthesis have been also identified and their pivotal contribution in oleogenesis has been discussed. To date, this study represents the first characterization of the olive fruit proteome during development, providing new insights into fruit metabolism and oil accumulation process.Linda BiancoFiammetta AlagnaLuciana BaldoniChristine FinnieBirte SvenssonGaetano PerrottaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53563 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Linda Bianco Fiammetta Alagna Luciana Baldoni Christine Finnie Birte Svensson Gaetano Perrotta Proteome regulation during Olea europaea fruit development.
description	<h4>Background</h4>Widespread in the Mediterranean basin, Olea europaea trees are gaining worldwide popularity for the nutritional and cancer-protective properties of the oil, mechanically extracted from ripe fruits. Fruit development is a physiological process with remarkable impact on the modulation of the biosynthesis of compounds affecting the quality of the drupes as well as the final composition of the olive oil. Proteomics offers the possibility to dig deeper into the major changes during fruit development, including the important phase of ripening, and to classify temporal patterns of protein accumulation occurring during these complex physiological processes.<h4>Methodology/principal findings</h4>In this work, we started monitoring the proteome variations associated with olive fruit development by using comparative proteomics coupled to mass spectrometry. Proteins extracted from drupes at three different developmental stages were separated on 2-DE and subjected to image analysis. 247 protein spots were revealed as differentially accumulated. Proteins were identified from a total of 121 spots and discussed in relation to olive drupe metabolic changes occurring during fruit development. In order to evaluate if changes observed at the protein level were consistent with changes of mRNAs, proteomic data produced in the present work were compared with transcriptomic data elaborated during previous studies.<h4>Conclusions/significance</h4>This study identifies a number of proteins responsible for quality traits of cv. Coratina, with particular regard to proteins associated to the metabolism of fatty acids, phenolic and aroma compounds. Proteins involved in fruit photosynthesis have been also identified and their pivotal contribution in oleogenesis has been discussed. To date, this study represents the first characterization of the olive fruit proteome during development, providing new insights into fruit metabolism and oil accumulation process.
format	article
author	Linda Bianco Fiammetta Alagna Luciana Baldoni Christine Finnie Birte Svensson Gaetano Perrotta
author_facet	Linda Bianco Fiammetta Alagna Luciana Baldoni Christine Finnie Birte Svensson Gaetano Perrotta
author_sort	Linda Bianco
title	Proteome regulation during Olea europaea fruit development.
title_short	Proteome regulation during Olea europaea fruit development.
title_full	Proteome regulation during Olea europaea fruit development.
title_fullStr	Proteome regulation during Olea europaea fruit development.
title_full_unstemmed	Proteome regulation during Olea europaea fruit development.
title_sort	proteome regulation during olea europaea fruit development.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b0d1613e988e4067813f2d33020c1a2e
work_keys_str_mv	AT lindabianco proteomeregulationduringoleaeuropaeafruitdevelopment AT fiammettaalagna proteomeregulationduringoleaeuropaeafruitdevelopment AT lucianabaldoni proteomeregulationduringoleaeuropaeafruitdevelopment AT christinefinnie proteomeregulationduringoleaeuropaeafruitdevelopment AT birtesvensson proteomeregulationduringoleaeuropaeafruitdevelopment AT gaetanoperrotta proteomeregulationduringoleaeuropaeafruitdevelopment
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Proteome regulation during Olea europaea fruit development.

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