Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells

Hongmei Jing1, Jing Wang1, Ping Yang1, Xiaoyan Ke1, Guohua Xia2, Baoan Chen21Department of Hematology and Lymphoma Research Center, Peking University Third Hospital, Beijing, People&rsquo;s Republic of China; 2Department of Hematology, The Affiliated Zhongda Hospital, Clinical Medical School...

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Autores principales:	Hongmei Jing, Jing Wang, Ping Yang, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b0d2894865614ee982774d6e46063a3f
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spelling	oai:doaj.org-article:b0d2894865614ee982774d6e46063a3f2021-12-02T02:14:38ZMagnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells1176-91141178-2013https://doaj.org/article/b0d2894865614ee982774d6e46063a3f2010-11-01T00:00:00Zhttp://www.dovepress.com/magnetic-fe3o4-nanoparticles-and-chemotherapy-agents-interact-synergis-a5693https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hongmei Jing1, Jing Wang1, Ping Yang1, Xiaoyan Ke1, Guohua Xia2, Baoan Chen21Department of Hematology and Lymphoma Research Center, Peking University Third Hospital, Beijing, People&rsquo;s Republic of China; 2Department of Hematology, The Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsquo;s Republic of ChinaAbstract: The purpose of this study was to investigate the potential effects of combination therapy using magnetic nanoparticles of Fe3O4 (MNP-Fe3O4) and chemotherapeutic drugs on lymphoma cells. Proliferation, inhibition, and viability of Raji cells were detected by MTT and trypan blue exclusion. The percentage of cells undergoing apoptosis was detected by flow cytometry using fluorescein isothiocyanate-annexin V and propidium iodide staining. p53 and nuclear factor-&kappa;B (NF-&kappa;B) protein levels were measured by Western blot. The results showed that proliferation of Raji cells was inhibited by adriamycin or daunorubicin in a dose-and time-dependent manner. Cell sensitivity was improved and the 50% inhibitory concentrations of adriamycin and daunorubicin decreased when combined with a MNP-Fe3O4 carrier. Interestingly, increased apoptosis in Raji lymphoma cells was accompanied by upregulation of p53 protein and downregulation of NF-&kappa;B protein. Furthermore, the combination of MNP-Fe3O4 with adriamycin or daunorubicin increased p53 protein levels and decreased NF-&kappa;B protein levels more than adriamycin or daunorubicin alone, indicating that MNP-Fe3O4 could enhance the effect of chemotherapeutic drugs on p53 and NF-&kappa;B. Similar results for cell apoptosis and protein expression were not observed for the groups treated with dexamethasone &plusmn; MNP-Fe3O4 (P &gt;0.05). These findings suggest a potential clinical application for MNP-Fe3O4 in combination with daunorubicin or adriamycin in the treatment of lymphoma.Keywords: magnetic nanoparticles, Raji cells, apoptosis, p53, NF-&kappa;B Hongmei JingJing WangPing Yanget alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 999-1004 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hongmei Jing Jing Wang Ping Yang et al Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
description	Hongmei Jing1, Jing Wang1, Ping Yang1, Xiaoyan Ke1, Guohua Xia2, Baoan Chen21Department of Hematology and Lymphoma Research Center, Peking University Third Hospital, Beijing, People&rsquo;s Republic of China; 2Department of Hematology, The Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People&rsquo;s Republic of ChinaAbstract: The purpose of this study was to investigate the potential effects of combination therapy using magnetic nanoparticles of Fe3O4 (MNP-Fe3O4) and chemotherapeutic drugs on lymphoma cells. Proliferation, inhibition, and viability of Raji cells were detected by MTT and trypan blue exclusion. The percentage of cells undergoing apoptosis was detected by flow cytometry using fluorescein isothiocyanate-annexin V and propidium iodide staining. p53 and nuclear factor-&kappa;B (NF-&kappa;B) protein levels were measured by Western blot. The results showed that proliferation of Raji cells was inhibited by adriamycin or daunorubicin in a dose-and time-dependent manner. Cell sensitivity was improved and the 50% inhibitory concentrations of adriamycin and daunorubicin decreased when combined with a MNP-Fe3O4 carrier. Interestingly, increased apoptosis in Raji lymphoma cells was accompanied by upregulation of p53 protein and downregulation of NF-&kappa;B protein. Furthermore, the combination of MNP-Fe3O4 with adriamycin or daunorubicin increased p53 protein levels and decreased NF-&kappa;B protein levels more than adriamycin or daunorubicin alone, indicating that MNP-Fe3O4 could enhance the effect of chemotherapeutic drugs on p53 and NF-&kappa;B. Similar results for cell apoptosis and protein expression were not observed for the groups treated with dexamethasone &plusmn; MNP-Fe3O4 (P &gt;0.05). These findings suggest a potential clinical application for MNP-Fe3O4 in combination with daunorubicin or adriamycin in the treatment of lymphoma.Keywords: magnetic nanoparticles, Raji cells, apoptosis, p53, NF-&kappa;B
format	article
author	Hongmei Jing Jing Wang Ping Yang et al
author_facet	Hongmei Jing Jing Wang Ping Yang et al
author_sort	Hongmei Jing
title	Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
title_short	Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
title_full	Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
title_fullStr	Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
title_full_unstemmed	Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
title_sort	magnetic fe3o4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/b0d2894865614ee982774d6e46063a3f
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_version_	1718402615209885696

Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells

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