Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose

Abstract In this study, we reported the development of Prussian blue (PB), poly(pyrrole-2-carboxylic acid) (PPCA), and glucose oxidase (GOx) biocomposite modified graphite rod (GR) electrode as a potential biocathode for single enzyme biofuel cell fuelled by glucose. In order to design the biocathod...

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Autores principales:	Asta Kausaite-Minkstimiene, Algimantas Kaminskas, Anton Popov, Arunas Ramanavicius, Almira Ramanaviciene
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0d3eee43cbb444bb5ae24d002787f6d
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spelling	oai:doaj.org-article:b0d3eee43cbb444bb5ae24d002787f6d2021-12-02T18:49:53ZDevelopment of a new biocathode for a single enzyme biofuel cell fuelled by glucose10.1038/s41598-021-97488-w2045-2322https://doaj.org/article/b0d3eee43cbb444bb5ae24d002787f6d2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97488-whttps://doaj.org/toc/2045-2322Abstract In this study, we reported the development of Prussian blue (PB), poly(pyrrole-2-carboxylic acid) (PPCA), and glucose oxidase (GOx) biocomposite modified graphite rod (GR) electrode as a potential biocathode for single enzyme biofuel cell fuelled by glucose. In order to design the biocathode, the GR electrode was coated with a composite of PB particles embedded in the PPCA shell and an additional layer of PPCA by cyclic voltammetry. Meanwhile, GOx molecules were covalently attached to the carboxyl groups of PPCA by an amide bond. The optimal conditions for the biocathode preparation were elaborated experimentally. After optimization, the developed biocathode showed excellent electrocatalytic activity toward the reduction of H2O2 formed during GOx catalyzed glucose oxidation at a low potential of 0.1 V vs Ag/AgCl, as well as good electrochemical performance. An electrocatalytic current density of 31.68 ± 2.70 μA/cm2 and open-circuit potential (OCP) of 293.34 ± 15.70 mV in O2-saturated 10 mM glucose solution at pH 6.0 were recorded. A maximal OCP of 430.15 ± 15.10 mV was recorded at 98.86 mM of glucose. In addition, the biocathode showed good operational stability, maintaining 95.53 ± 0.15% of the initial response after 14 days. These results suggest that this simply designed biocathode can be applied to the construction of a glucose-powered single enzyme biofuel cell.Asta Kausaite-MinkstimieneAlgimantas KaminskasAnton PopovArunas RamanaviciusAlmira RamanavicieneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Asta Kausaite-Minkstimiene Algimantas Kaminskas Anton Popov Arunas Ramanavicius Almira Ramanaviciene Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose
description	Abstract In this study, we reported the development of Prussian blue (PB), poly(pyrrole-2-carboxylic acid) (PPCA), and glucose oxidase (GOx) biocomposite modified graphite rod (GR) electrode as a potential biocathode for single enzyme biofuel cell fuelled by glucose. In order to design the biocathode, the GR electrode was coated with a composite of PB particles embedded in the PPCA shell and an additional layer of PPCA by cyclic voltammetry. Meanwhile, GOx molecules were covalently attached to the carboxyl groups of PPCA by an amide bond. The optimal conditions for the biocathode preparation were elaborated experimentally. After optimization, the developed biocathode showed excellent electrocatalytic activity toward the reduction of H2O2 formed during GOx catalyzed glucose oxidation at a low potential of 0.1 V vs Ag/AgCl, as well as good electrochemical performance. An electrocatalytic current density of 31.68 ± 2.70 μA/cm2 and open-circuit potential (OCP) of 293.34 ± 15.70 mV in O2-saturated 10 mM glucose solution at pH 6.0 were recorded. A maximal OCP of 430.15 ± 15.10 mV was recorded at 98.86 mM of glucose. In addition, the biocathode showed good operational stability, maintaining 95.53 ± 0.15% of the initial response after 14 days. These results suggest that this simply designed biocathode can be applied to the construction of a glucose-powered single enzyme biofuel cell.
format	article
author	Asta Kausaite-Minkstimiene Algimantas Kaminskas Anton Popov Arunas Ramanavicius Almira Ramanaviciene
author_facet	Asta Kausaite-Minkstimiene Algimantas Kaminskas Anton Popov Arunas Ramanavicius Almira Ramanaviciene
author_sort	Asta Kausaite-Minkstimiene
title	Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose
title_short	Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose
title_full	Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose
title_fullStr	Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose
title_full_unstemmed	Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose
title_sort	development of a new biocathode for a single enzyme biofuel cell fuelled by glucose
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0d3eee43cbb444bb5ae24d002787f6d
work_keys_str_mv	AT astakausaiteminkstimiene developmentofanewbiocathodeforasingleenzymebiofuelcellfuelledbyglucose AT algimantaskaminskas developmentofanewbiocathodeforasingleenzymebiofuelcellfuelledbyglucose AT antonpopov developmentofanewbiocathodeforasingleenzymebiofuelcellfuelledbyglucose AT arunasramanavicius developmentofanewbiocathodeforasingleenzymebiofuelcellfuelledbyglucose AT almiraramanaviciene developmentofanewbiocathodeforasingleenzymebiofuelcellfuelledbyglucose
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Development of a new biocathode for a single enzyme biofuel cell fuelled by glucose

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