Melanocyte progenitor cells reside in human subcutaneous adipose tissue.

Based on the assumption that some progenitor cells in an organ might reside in neighboring adipose tissue, we investigated whether melanocyte progenitor cells reside in human subcutaneous adipose tissue. First, we examined the expression of human melanoma black 45 (HMB45) and microphthalmia-associat...

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Autores principales:	Yuri Ikeda, Akino Wada, Toshio Hasegawa, Mutsumi Yokota, Masato Koike, Shigaku Ikeda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0d46761328c4aab8a4f6b97e9d6d7ff
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spelling	oai:doaj.org-article:b0d46761328c4aab8a4f6b97e9d6d7ff2021-12-02T20:19:35ZMelanocyte progenitor cells reside in human subcutaneous adipose tissue.1932-620310.1371/journal.pone.0256622https://doaj.org/article/b0d46761328c4aab8a4f6b97e9d6d7ff2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256622https://doaj.org/toc/1932-6203Based on the assumption that some progenitor cells in an organ might reside in neighboring adipose tissue, we investigated whether melanocyte progenitor cells reside in human subcutaneous adipose tissue. First, we examined the expression of human melanoma black 45 (HMB45) and microphthalmia-associated transcription factor (MITF) in undifferentiated adipose-derived stem cells (ADSCs) by immunostaining, RT-PCR, and western blotting. These two markers were detected in undifferentiated ADSCs, and their expression levels were increased in differentiated ADSCs in melanocyte-specific culture medium. Other melanocytic markers (Melan A, MATP, Mel2, Mel EM, tyrosinase, KIT, and PAX3) were also detected at variable levels in undifferentiated ADSCs, and the expression of some markers was increased during differentiation into the melanocyte lineage. We further showed that ADSCs differentiated in melanocyte-specific culture medium localized in the basal layer and expressed tyrosinase and HMB45 in a 3D epidermal culture system. Melanin deposits were also induced by ultraviolet-light-B (UVB) irradiation. These results demonstrate that melanocyte progenitor cells reside in human subcutaneous adipose tissue and that these cells might have the potential to differentiate into mature melanocytes. Melanocyte and keratinocyte progenitors residing in human subcutaneous tissue can be used for the treatment of skin diseases and skin rejuvenation in the future.Yuri IkedaAkino WadaToshio HasegawaMutsumi YokotaMasato KoikeShigaku IkedaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256622 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuri Ikeda Akino Wada Toshio Hasegawa Mutsumi Yokota Masato Koike Shigaku Ikeda Melanocyte progenitor cells reside in human subcutaneous adipose tissue.
description	Based on the assumption that some progenitor cells in an organ might reside in neighboring adipose tissue, we investigated whether melanocyte progenitor cells reside in human subcutaneous adipose tissue. First, we examined the expression of human melanoma black 45 (HMB45) and microphthalmia-associated transcription factor (MITF) in undifferentiated adipose-derived stem cells (ADSCs) by immunostaining, RT-PCR, and western blotting. These two markers were detected in undifferentiated ADSCs, and their expression levels were increased in differentiated ADSCs in melanocyte-specific culture medium. Other melanocytic markers (Melan A, MATP, Mel2, Mel EM, tyrosinase, KIT, and PAX3) were also detected at variable levels in undifferentiated ADSCs, and the expression of some markers was increased during differentiation into the melanocyte lineage. We further showed that ADSCs differentiated in melanocyte-specific culture medium localized in the basal layer and expressed tyrosinase and HMB45 in a 3D epidermal culture system. Melanin deposits were also induced by ultraviolet-light-B (UVB) irradiation. These results demonstrate that melanocyte progenitor cells reside in human subcutaneous adipose tissue and that these cells might have the potential to differentiate into mature melanocytes. Melanocyte and keratinocyte progenitors residing in human subcutaneous tissue can be used for the treatment of skin diseases and skin rejuvenation in the future.
format	article
author	Yuri Ikeda Akino Wada Toshio Hasegawa Mutsumi Yokota Masato Koike Shigaku Ikeda
author_facet	Yuri Ikeda Akino Wada Toshio Hasegawa Mutsumi Yokota Masato Koike Shigaku Ikeda
author_sort	Yuri Ikeda
title	Melanocyte progenitor cells reside in human subcutaneous adipose tissue.
title_short	Melanocyte progenitor cells reside in human subcutaneous adipose tissue.
title_full	Melanocyte progenitor cells reside in human subcutaneous adipose tissue.
title_fullStr	Melanocyte progenitor cells reside in human subcutaneous adipose tissue.
title_full_unstemmed	Melanocyte progenitor cells reside in human subcutaneous adipose tissue.
title_sort	melanocyte progenitor cells reside in human subcutaneous adipose tissue.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b0d46761328c4aab8a4f6b97e9d6d7ff
work_keys_str_mv	AT yuriikeda melanocyteprogenitorcellsresideinhumansubcutaneousadiposetissue AT akinowada melanocyteprogenitorcellsresideinhumansubcutaneousadiposetissue AT toshiohasegawa melanocyteprogenitorcellsresideinhumansubcutaneousadiposetissue AT mutsumiyokota melanocyteprogenitorcellsresideinhumansubcutaneousadiposetissue AT masatokoike melanocyteprogenitorcellsresideinhumansubcutaneousadiposetissue AT shigakuikeda melanocyteprogenitorcellsresideinhumansubcutaneousadiposetissue
_version_	1718374185588228096

Melanocyte progenitor cells reside in human subcutaneous adipose tissue.

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