Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro

Myocardial tissue undergoes steady functional decline when cultured in vitro. Here, the authors report a protocol for culture of human cardiac slices that allows maintenance of contractility for up to four months, and show that the model is suitable for evaluation of drug safety, as exemplified for...

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Autores principales:	Carola Fischer, Hendrik Milting, Evelyn Fein, Elisabeth Reiser, Kun Lu, Thomas Seidel, Camilla Schinner, Thomas Schwarzmayr, Rene Schramm, Roland Tomasi, Britta Husse, Xiaochun Cao-Ehlker, Ulrich Pohl, Andreas Dendorfer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b0d54360a0484bdfbbb91bce79c8f903
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spelling	oai:doaj.org-article:b0d54360a0484bdfbbb91bce79c8f9032021-12-02T17:01:31ZLong-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro10.1038/s41467-018-08003-12041-1723https://doaj.org/article/b0d54360a0484bdfbbb91bce79c8f9032019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08003-1https://doaj.org/toc/2041-1723Myocardial tissue undergoes steady functional decline when cultured in vitro. Here, the authors report a protocol for culture of human cardiac slices that allows maintenance of contractility for up to four months, and show that the model is suitable for evaluation of drug safety, as exemplified for drugs interfering with cardiomyocyte repolarization.Carola FischerHendrik MiltingEvelyn FeinElisabeth ReiserKun LuThomas SeidelCamilla SchinnerThomas SchwarzmayrRene SchrammRoland TomasiBritta HusseXiaochun Cao-EhlkerUlrich PohlAndreas DendorferNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Carola Fischer Hendrik Milting Evelyn Fein Elisabeth Reiser Kun Lu Thomas Seidel Camilla Schinner Thomas Schwarzmayr Rene Schramm Roland Tomasi Britta Husse Xiaochun Cao-Ehlker Ulrich Pohl Andreas Dendorfer Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro
description	Myocardial tissue undergoes steady functional decline when cultured in vitro. Here, the authors report a protocol for culture of human cardiac slices that allows maintenance of contractility for up to four months, and show that the model is suitable for evaluation of drug safety, as exemplified for drugs interfering with cardiomyocyte repolarization.
format	article
author	Carola Fischer Hendrik Milting Evelyn Fein Elisabeth Reiser Kun Lu Thomas Seidel Camilla Schinner Thomas Schwarzmayr Rene Schramm Roland Tomasi Britta Husse Xiaochun Cao-Ehlker Ulrich Pohl Andreas Dendorfer
author_facet	Carola Fischer Hendrik Milting Evelyn Fein Elisabeth Reiser Kun Lu Thomas Seidel Camilla Schinner Thomas Schwarzmayr Rene Schramm Roland Tomasi Britta Husse Xiaochun Cao-Ehlker Ulrich Pohl Andreas Dendorfer
author_sort	Carola Fischer
title	Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro
title_short	Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro
title_full	Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro
title_fullStr	Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro
title_full_unstemmed	Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro
title_sort	long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/b0d54360a0484bdfbbb91bce79c8f903
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Long-term functional and structural preservation of precision-cut human myocardium under continuous electromechanical stimulation in vitro

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