Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.

The present study demonstrates exopolysaccharide production by an osmotolerant marine isolate and also describes further application of the purified polysaccharide for production of colloidal suspension of silver nanoparticles with narrow size distribution. Phylogenetic analysis based on 16S r RNA g...

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Autores principales:	Ananya Mehta, Chandni Sidhu, Anil Kumar Pinnaka, Anirban Roy Choudhury
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0d8ffab1e8a4879b5a6902dc028e540
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spelling	oai:doaj.org-article:b0d8ffab1e8a4879b5a6902dc028e5402021-11-18T08:15:31ZExtracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.1932-620310.1371/journal.pone.0098798https://doaj.org/article/b0d8ffab1e8a4879b5a6902dc028e5402014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24932690/?tool=EBIhttps://doaj.org/toc/1932-6203The present study demonstrates exopolysaccharide production by an osmotolerant marine isolate and also describes further application of the purified polysaccharide for production of colloidal suspension of silver nanoparticles with narrow size distribution. Phylogenetic analysis based on 16S r RNA gene sequencing revealed close affinity of the isolate to Alteromonas macleodii. Unlike earlier reports, where glucose was used as the carbon source, lactose was found to be the most suitable substrate for polysaccharide production. The strain was capable of producing 23.4 gl(-1) exopolysaccharide with a productivity of 7.8 gl(-1) day(-1) when 15% (w/v) lactose was used as carbon source. Furthermore, the purified polysaccharide was able to produce spherical shaped silver nanoparticles of around 70 nm size as characterized by Uv-vis spectroscopy, Dynamic light scattering and Transmission electron microscopy. These observations suggested possible commercial potential of the isolated strain for production of a polysaccharide which has the capability of synthesizing biocompatible metal nanoparticle.Ananya MehtaChandni SidhuAnil Kumar PinnakaAnirban Roy ChoudhuryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98798 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ananya Mehta Chandni Sidhu Anil Kumar Pinnaka Anirban Roy Choudhury Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.
description	The present study demonstrates exopolysaccharide production by an osmotolerant marine isolate and also describes further application of the purified polysaccharide for production of colloidal suspension of silver nanoparticles with narrow size distribution. Phylogenetic analysis based on 16S r RNA gene sequencing revealed close affinity of the isolate to Alteromonas macleodii. Unlike earlier reports, where glucose was used as the carbon source, lactose was found to be the most suitable substrate for polysaccharide production. The strain was capable of producing 23.4 gl(-1) exopolysaccharide with a productivity of 7.8 gl(-1) day(-1) when 15% (w/v) lactose was used as carbon source. Furthermore, the purified polysaccharide was able to produce spherical shaped silver nanoparticles of around 70 nm size as characterized by Uv-vis spectroscopy, Dynamic light scattering and Transmission electron microscopy. These observations suggested possible commercial potential of the isolated strain for production of a polysaccharide which has the capability of synthesizing biocompatible metal nanoparticle.
format	article
author	Ananya Mehta Chandni Sidhu Anil Kumar Pinnaka Anirban Roy Choudhury
author_facet	Ananya Mehta Chandni Sidhu Anil Kumar Pinnaka Anirban Roy Choudhury
author_sort	Ananya Mehta
title	Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.
title_short	Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.
title_full	Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.
title_fullStr	Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.
title_full_unstemmed	Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.
title_sort	extracellular polysaccharide production by a novel osmotolerant marine strain of alteromonas macleodii and its application towards biomineralization of silver.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b0d8ffab1e8a4879b5a6902dc028e540
work_keys_str_mv	AT ananyamehta extracellularpolysaccharideproductionbyanovelosmotolerantmarinestrainofalteromonasmacleodiianditsapplicationtowardsbiomineralizationofsilver AT chandnisidhu extracellularpolysaccharideproductionbyanovelosmotolerantmarinestrainofalteromonasmacleodiianditsapplicationtowardsbiomineralizationofsilver AT anilkumarpinnaka extracellularpolysaccharideproductionbyanovelosmotolerantmarinestrainofalteromonasmacleodiianditsapplicationtowardsbiomineralizationofsilver AT anirbanroychoudhury extracellularpolysaccharideproductionbyanovelosmotolerantmarinestrainofalteromonasmacleodiianditsapplicationtowardsbiomineralizationofsilver
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Extracellular polysaccharide production by a novel osmotolerant marine strain of Alteromonas macleodii and its application towards biomineralization of silver.

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