Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields

Uniform illumination is a prerequisite for quantitative analyses in both classical fluorescence microscopy and single molecule localisation microscopy. Here, the authors introduce ASTER, an illumination technique that generates uniform illumination over large and adaptable fields of view, compatible...

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Main Authors:	Adrien Mau, Karoline Friedl, Christophe Leterrier, Nicolas Bourg, Sandrine Lévêque-Fort
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Science Q
Online Access:	https://doaj.org/article/b0da31c8ca7846b6baa6e7f45e1c492d
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spelling	oai:doaj.org-article:b0da31c8ca7846b6baa6e7f45e1c492d2021-12-02T16:53:15ZFast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields10.1038/s41467-021-23405-42041-1723https://doaj.org/article/b0da31c8ca7846b6baa6e7f45e1c492d2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23405-4https://doaj.org/toc/2041-1723Uniform illumination is a prerequisite for quantitative analyses in both classical fluorescence microscopy and single molecule localisation microscopy. Here, the authors introduce ASTER, an illumination technique that generates uniform illumination over large and adaptable fields of view, compatible with epifluorescence, HiLo and TIRF illumination schemes.Adrien MauKaroline FriedlChristophe LeterrierNicolas BourgSandrine Lévêque-FortNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Adrien Mau Karoline Friedl Christophe Leterrier Nicolas Bourg Sandrine Lévêque-Fort Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
description	Uniform illumination is a prerequisite for quantitative analyses in both classical fluorescence microscopy and single molecule localisation microscopy. Here, the authors introduce ASTER, an illumination technique that generates uniform illumination over large and adaptable fields of view, compatible with epifluorescence, HiLo and TIRF illumination schemes.
format	article
author	Adrien Mau Karoline Friedl Christophe Leterrier Nicolas Bourg Sandrine Lévêque-Fort
author_facet	Adrien Mau Karoline Friedl Christophe Leterrier Nicolas Bourg Sandrine Lévêque-Fort
author_sort	Adrien Mau
title	Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
title_short	Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
title_full	Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
title_fullStr	Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
title_full_unstemmed	Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
title_sort	fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0da31c8ca7846b6baa6e7f45e1c492d
work_keys_str_mv	AT adrienmau fastwidefieldscanprovidestunableanduniformilluminationoptimizingsuperresolutionmicroscopyonlargefields AT karolinefriedl fastwidefieldscanprovidestunableanduniformilluminationoptimizingsuperresolutionmicroscopyonlargefields AT christopheleterrier fastwidefieldscanprovidestunableanduniformilluminationoptimizingsuperresolutionmicroscopyonlargefields AT nicolasbourg fastwidefieldscanprovidestunableanduniformilluminationoptimizingsuperresolutionmicroscopyonlargefields AT sandrinelevequefort fastwidefieldscanprovidestunableanduniformilluminationoptimizingsuperresolutionmicroscopyonlargefields
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Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields

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