The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)

The spleen is an important organ in the immune function of fish, and it is also important for hematogenesis and antibody and granulocyte production. However, the effect of oxidized fish oil on the spleen of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus) is unknown. In this...

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Autores principales:	Shuisheng Long, Zhihao Li, Xiaohui Dong, Xiaobo Yan, Hao Liu, Beiping Tan, Shuang Zhang, Simiao Pan, Tao Li, Xiangxiang Suo, Yuanzhi Yang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	hybrid grouper (♀ Epinephelus fuscoguttatus × ♀E. lanceolatus) oxidized fish oil spleen oxidative stress antioxidant ability transcriptome Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/b0db4f2599984c68b37b9c7fdf9daa31
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spelling	oai:doaj.org-article:b0db4f2599984c68b37b9c7fdf9daa312021-11-30T13:45:54ZThe Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)2296-774510.3389/fmars.2021.779305https://doaj.org/article/b0db4f2599984c68b37b9c7fdf9daa312021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.779305/fullhttps://doaj.org/toc/2296-7745The spleen is an important organ in the immune function of fish, and it is also important for hematogenesis and antibody and granulocyte production. However, the effect of oxidized fish oil on the spleen of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus) is unknown. In this study, hybrid groupers were fed with oxidized fish oil and the spleen index, antioxidant ability, histology and transcriptome were investigated. Oxidized fish oil did not affect the spleen index. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the spleen were significantly increased as the amount of oxidized fish oil in the diet increased, but the vitamin E concentration was significantly decreased. The morphological organization of the spleen was damaged with increased oxidative stress. And the spleen reacted to oxidative stress by platelet activation, FOXO and notch signaling pathways, which involved amyloid beta precursor protein binding family B member 1 interacting protein (APBB1IP) gene, glucose-6-phosphatase (G6PC) gene, histone acetyltransferase p300 (EP300) gene, insulin gene and notch 2 gene. In conclusion, the oxidized fish oil caused oxidative stress and damaged its structure. Additionally, oxidized fish oil changed the transcription profile of the spleen.Shuisheng LongShuisheng LongZhihao LiZhihao LiXiaohui DongXiaohui DongXiaohui DongXiaobo YanXiaobo YanHao LiuHao LiuBeiping TanBeiping TanBeiping TanShuang ZhangShuang ZhangShuang ZhangSimiao PanSimiao PanTao LiTao LiXiangxiang SuoXiangxiang SuoYuanzhi YangFrontiers Media S.A.articlehybrid grouper (♀ Epinephelus fuscoguttatus × ♀E. lanceolatus)oxidized fish oilspleenoxidative stressantioxidant abilitytranscriptomeScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hybrid grouper (♀ Epinephelus fuscoguttatus × ♀E. lanceolatus) oxidized fish oil spleen oxidative stress antioxidant ability transcriptome Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	hybrid grouper (♀ Epinephelus fuscoguttatus × ♀E. lanceolatus) oxidized fish oil spleen oxidative stress antioxidant ability transcriptome Science Q General. Including nature conservation, geographical distribution QH1-199.5 Shuisheng Long Shuisheng Long Zhihao Li Zhihao Li Xiaohui Dong Xiaohui Dong Xiaohui Dong Xiaobo Yan Xiaobo Yan Hao Liu Hao Liu Beiping Tan Beiping Tan Beiping Tan Shuang Zhang Shuang Zhang Shuang Zhang Simiao Pan Simiao Pan Tao Li Tao Li Xiangxiang Suo Xiangxiang Suo Yuanzhi Yang The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)
description	The spleen is an important organ in the immune function of fish, and it is also important for hematogenesis and antibody and granulocyte production. However, the effect of oxidized fish oil on the spleen of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus) is unknown. In this study, hybrid groupers were fed with oxidized fish oil and the spleen index, antioxidant ability, histology and transcriptome were investigated. Oxidized fish oil did not affect the spleen index. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the spleen were significantly increased as the amount of oxidized fish oil in the diet increased, but the vitamin E concentration was significantly decreased. The morphological organization of the spleen was damaged with increased oxidative stress. And the spleen reacted to oxidative stress by platelet activation, FOXO and notch signaling pathways, which involved amyloid beta precursor protein binding family B member 1 interacting protein (APBB1IP) gene, glucose-6-phosphatase (G6PC) gene, histone acetyltransferase p300 (EP300) gene, insulin gene and notch 2 gene. In conclusion, the oxidized fish oil caused oxidative stress and damaged its structure. Additionally, oxidized fish oil changed the transcription profile of the spleen.
format	article
author	Shuisheng Long Shuisheng Long Zhihao Li Zhihao Li Xiaohui Dong Xiaohui Dong Xiaohui Dong Xiaobo Yan Xiaobo Yan Hao Liu Hao Liu Beiping Tan Beiping Tan Beiping Tan Shuang Zhang Shuang Zhang Shuang Zhang Simiao Pan Simiao Pan Tao Li Tao Li Xiangxiang Suo Xiangxiang Suo Yuanzhi Yang
author_facet	Shuisheng Long Shuisheng Long Zhihao Li Zhihao Li Xiaohui Dong Xiaohui Dong Xiaohui Dong Xiaobo Yan Xiaobo Yan Hao Liu Hao Liu Beiping Tan Beiping Tan Beiping Tan Shuang Zhang Shuang Zhang Shuang Zhang Simiao Pan Simiao Pan Tao Li Tao Li Xiangxiang Suo Xiangxiang Suo Yuanzhi Yang
author_sort	Shuisheng Long
title	The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)
title_short	The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)
title_full	The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)
title_fullStr	The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)
title_full_unstemmed	The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)
title_sort	effect of oxidized fish oil on the spleen index, antioxidant activity, histology and transcriptome in juvenile hybrid grouper (♀ epinephelus fuscoguttatus × ♂ epinephelus lanceolatus)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b0db4f2599984c68b37b9c7fdf9daa31
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The Effect of Oxidized Fish Oil on the Spleen Index, Antioxidant Activity, Histology and Transcriptome in Juvenile Hybrid Grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)

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