Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis

Abstract Infectious keratitis is a potentially sight threatening ophthalmological emergency. Contact lens wear is a common risk factor. Diagnostic advances such as MALDI-TOF MS provides new insights into the spectrum of corneal pathogens and on microbes previously considered as commensals. Corynebac...

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Autores principales:	Susanna Sagerfors, Anja Poehlein, Mastaneh Afshar, Birgitta Ejdervik Lindblad, Holger Brüggemann, Bo Söderquist
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0e1d2813d824aa8983f3f09775001da
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spelling	oai:doaj.org-article:b0e1d2813d824aa8983f3f09775001da2021-12-02T17:05:46ZClinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis10.1038/s41598-021-85336-w2045-2322https://doaj.org/article/b0e1d2813d824aa8983f3f09775001da2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85336-whttps://doaj.org/toc/2045-2322Abstract Infectious keratitis is a potentially sight threatening ophthalmological emergency. Contact lens wear is a common risk factor. Diagnostic advances such as MALDI-TOF MS provides new insights into the spectrum of corneal pathogens and on microbes previously considered as commensals. Corynebacterium macginleyi was described in 1995, and in 2018, the genomic features of three isolates were reported after whole-genome sequencing. Here we describe the clinical characteristics of patients with infectious keratitis (n = 29) presumably caused by Corynebacterium macginleyi, and analyze the genomic features of C. macginleyi (n = 22) isolated from the corneal ulcers of these patients. The disease course was uneventful apart from minor interventions such as corneal cross-linking and amniotic membrane transplant. Genome sequencing and comparison revealed a highly conserved core genome of C. macginleyi. Based on the analyses of single nucleotide polymorphisms, the population could be divided into two main clades that also differed in a few clade-specific genomic islands. Patients infected with an isolate belonging to the minor clade (n = 7) presented a more severe disease. Comparisons with other corynebacterial species clearly separated C. macginleyi. C. macginleyi may be considered a corneal pathogen; genomic analysis provided insights into its population structure and disease-causing potential.Susanna SagerforsAnja PoehleinMastaneh AfsharBirgitta Ejdervik LindbladHolger BrüggemannBo SöderquistNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susanna Sagerfors Anja Poehlein Mastaneh Afshar Birgitta Ejdervik Lindblad Holger Brüggemann Bo Söderquist Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis
description	Abstract Infectious keratitis is a potentially sight threatening ophthalmological emergency. Contact lens wear is a common risk factor. Diagnostic advances such as MALDI-TOF MS provides new insights into the spectrum of corneal pathogens and on microbes previously considered as commensals. Corynebacterium macginleyi was described in 1995, and in 2018, the genomic features of three isolates were reported after whole-genome sequencing. Here we describe the clinical characteristics of patients with infectious keratitis (n = 29) presumably caused by Corynebacterium macginleyi, and analyze the genomic features of C. macginleyi (n = 22) isolated from the corneal ulcers of these patients. The disease course was uneventful apart from minor interventions such as corneal cross-linking and amniotic membrane transplant. Genome sequencing and comparison revealed a highly conserved core genome of C. macginleyi. Based on the analyses of single nucleotide polymorphisms, the population could be divided into two main clades that also differed in a few clade-specific genomic islands. Patients infected with an isolate belonging to the minor clade (n = 7) presented a more severe disease. Comparisons with other corynebacterial species clearly separated C. macginleyi. C. macginleyi may be considered a corneal pathogen; genomic analysis provided insights into its population structure and disease-causing potential.
format	article
author	Susanna Sagerfors Anja Poehlein Mastaneh Afshar Birgitta Ejdervik Lindblad Holger Brüggemann Bo Söderquist
author_facet	Susanna Sagerfors Anja Poehlein Mastaneh Afshar Birgitta Ejdervik Lindblad Holger Brüggemann Bo Söderquist
author_sort	Susanna Sagerfors
title	Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis
title_short	Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis
title_full	Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis
title_fullStr	Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis
title_full_unstemmed	Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis
title_sort	clinical and genomic features of corynebacterium macginleyi-associated infectious keratitis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0e1d2813d824aa8983f3f09775001da
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Clinical and genomic features of Corynebacterium macginleyi-associated infectious keratitis

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