Identification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis
<i>Fusarium</i> wilt (<i>FW</i>) in cucumber (<i>Cucumis sativus</i> L.), caused by <i>Fusarium oxysporum</i> f. sp. cucumerinum (Foc), poses a major threat to cucumber growth and productivity. However, lack of available natural resistance resources fo...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/b0e7bd23616b480da757651837239f85 |
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| Sumario: | <i>Fusarium</i> wilt (<i>FW</i>) in cucumber (<i>Cucumis sativus</i> L.), caused by <i>Fusarium oxysporum</i> f. sp. cucumerinum (Foc), poses a major threat to cucumber growth and productivity. However, lack of available natural resistance resources for <i>FW</i> restricts the breeding of resistant cultivars via conventional approaches. Susceptibility (S) genes in susceptible host plants facilitate infection by the pathogen and contribute to susceptibility. Loss of function of these S genes might provide broad-spectrum and durable disease resistance. Here, we screened S genes via comparative proteomic analysis between cucumber cultivars Rijiecheng and Superina, which exhibited resistance and high -susceptibility to <i>FW</i>, respectively. We identified 210 and 243 differentially regulated proteins (DRPs) in the Rijiecheng and Superina, respectively, and further found that 32 DRPs were predominantly expressed in Superina and significantly up-regulated after Foc inoculation. Expression verification found that <i>TMEM115</i> (<i>CsaV3_5G025750</i>), encoding a transmembrane protein, <i>TET8</i> (<i>CsaV3_2G007840</i>), encoding function as a tetraspanin, <i>TPS10</i> (<i>CsaV3_2G017980</i>) encoding a terpene synthase, and <i>MGT2</i> (<i>CsaV3_7G006660</i>), encoding a glycosyltransferase, were significantly induced in both cultivars after Foc infection but were induced to a higher expression level in Superina. These candidate genes might act as negative regulators of <i>FW</i> resistance in cucumber and provide effective <i>FW</i>-susceptibility gene resources for improving cucumber <i>FW</i> resistance through breeding programs. |
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