Identification of Susceptibility Genes for Fusarium oxysporum in Cucumber via Comparative Proteomic Analysis

Fusarium wilt (FW) in cucumber (Cucumis sativus L.), caused by Fusarium oxysporum f. sp. cucumerinum (Foc), poses a major threat to cucumber growth and productivity. However, lack of available natural resistance resources fo...

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Autores principales:	Jun Xu, Ke Wang, Qianqian Xian, Ningyuan Zhang, Jingping Dong, Xuehao Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cucumber protein sequencing <i>Fusarium</i> wilt susceptibility genes Genetics QH426-470
Acceso en línea:	https://doaj.org/article/b0e7bd23616b480da757651837239f85
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spelling	oai:doaj.org-article:b0e7bd23616b480da757651837239f852021-11-25T17:41:55ZIdentification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis10.3390/genes121117812073-4425https://doaj.org/article/b0e7bd23616b480da757651837239f852021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1781https://doaj.org/toc/2073-4425<i>Fusarium</i> wilt (<i>FW</i>) in cucumber (<i>Cucumis sativus</i> L.), caused by <i>Fusarium oxysporum</i> f. sp. cucumerinum (Foc), poses a major threat to cucumber growth and productivity. However, lack of available natural resistance resources for <i>FW</i> restricts the breeding of resistant cultivars via conventional approaches. Susceptibility (S) genes in susceptible host plants facilitate infection by the pathogen and contribute to susceptibility. Loss of function of these S genes might provide broad-spectrum and durable disease resistance. Here, we screened S genes via comparative proteomic analysis between cucumber cultivars Rijiecheng and Superina, which exhibited resistance and high -susceptibility to <i>FW</i>, respectively. We identified 210 and 243 differentially regulated proteins (DRPs) in the Rijiecheng and Superina, respectively, and further found that 32 DRPs were predominantly expressed in Superina and significantly up-regulated after Foc inoculation. Expression verification found that <i>TMEM115</i> (<i>CsaV3_5G025750</i>), encoding a transmembrane protein, <i>TET8</i> (<i>CsaV3_2G007840</i>), encoding function as a tetraspanin, <i>TPS10</i> (<i>CsaV3_2G017980</i>) encoding a terpene synthase, and <i>MGT2</i> (<i>CsaV3_7G006660</i>), encoding a glycosyltransferase, were significantly induced in both cultivars after Foc infection but were induced to a higher expression level in Superina. These candidate genes might act as negative regulators of <i>FW</i> resistance in cucumber and provide effective <i>FW</i>-susceptibility gene resources for improving cucumber <i>FW</i> resistance through breeding programs.Jun XuKe WangQianqian XianNingyuan ZhangJingping DongXuehao ChenMDPI AGarticlecucumberprotein sequencing<i>Fusarium</i> wiltsusceptibility genesGeneticsQH426-470ENGenes, Vol 12, Iss 1781, p 1781 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cucumber protein sequencing <i>Fusarium</i> wilt susceptibility genes Genetics QH426-470
spellingShingle	cucumber protein sequencing <i>Fusarium</i> wilt susceptibility genes Genetics QH426-470 Jun Xu Ke Wang Qianqian Xian Ningyuan Zhang Jingping Dong Xuehao Chen Identification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis
description	<i>Fusarium</i> wilt (<i>FW</i>) in cucumber (<i>Cucumis sativus</i> L.), caused by <i>Fusarium oxysporum</i> f. sp. cucumerinum (Foc), poses a major threat to cucumber growth and productivity. However, lack of available natural resistance resources for <i>FW</i> restricts the breeding of resistant cultivars via conventional approaches. Susceptibility (S) genes in susceptible host plants facilitate infection by the pathogen and contribute to susceptibility. Loss of function of these S genes might provide broad-spectrum and durable disease resistance. Here, we screened S genes via comparative proteomic analysis between cucumber cultivars Rijiecheng and Superina, which exhibited resistance and high -susceptibility to <i>FW</i>, respectively. We identified 210 and 243 differentially regulated proteins (DRPs) in the Rijiecheng and Superina, respectively, and further found that 32 DRPs were predominantly expressed in Superina and significantly up-regulated after Foc inoculation. Expression verification found that <i>TMEM115</i> (<i>CsaV3_5G025750</i>), encoding a transmembrane protein, <i>TET8</i> (<i>CsaV3_2G007840</i>), encoding function as a tetraspanin, <i>TPS10</i> (<i>CsaV3_2G017980</i>) encoding a terpene synthase, and <i>MGT2</i> (<i>CsaV3_7G006660</i>), encoding a glycosyltransferase, were significantly induced in both cultivars after Foc infection but were induced to a higher expression level in Superina. These candidate genes might act as negative regulators of <i>FW</i> resistance in cucumber and provide effective <i>FW</i>-susceptibility gene resources for improving cucumber <i>FW</i> resistance through breeding programs.
format	article
author	Jun Xu Ke Wang Qianqian Xian Ningyuan Zhang Jingping Dong Xuehao Chen
author_facet	Jun Xu Ke Wang Qianqian Xian Ningyuan Zhang Jingping Dong Xuehao Chen
author_sort	Jun Xu
title	Identification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis
title_short	Identification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis
title_full	Identification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis
title_fullStr	Identification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis
title_full_unstemmed	Identification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis
title_sort	identification of susceptibility genes for <i>fusarium oxysporum</i> in cucumber via comparative proteomic analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b0e7bd23616b480da757651837239f85
work_keys_str_mv	AT junxu identificationofsusceptibilitygenesforifusariumoxysporumiincucumberviacomparativeproteomicanalysis AT kewang identificationofsusceptibilitygenesforifusariumoxysporumiincucumberviacomparativeproteomicanalysis AT qianqianxian identificationofsusceptibilitygenesforifusariumoxysporumiincucumberviacomparativeproteomicanalysis AT ningyuanzhang identificationofsusceptibilitygenesforifusariumoxysporumiincucumberviacomparativeproteomicanalysis AT jingpingdong identificationofsusceptibilitygenesforifusariumoxysporumiincucumberviacomparativeproteomicanalysis AT xuehaochen identificationofsusceptibilitygenesforifusariumoxysporumiincucumberviacomparativeproteomicanalysis
_version_	1718412125672570880

Identification of Susceptibility Genes for <i>Fusarium oxysporum</i> in Cucumber via Comparative Proteomic Analysis

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