Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60

Introduction. In recent years, due to wide applications of nanotechnologies in various fields, the safety of nanomaterials has become a pressing issue. Fullerene C60 is not an exception. Research on the activity of microorganisms and their interaction with nanoparticles is of major importance, both...

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Autores principales:	Olga POSTOLACHI, Inna RASTIMESINA, Valentina JOSAN
Formato:	article
Lenguaje:	EN FR RO RU
Publicado:	Asociatia de Biosiguranta si Biosecuritate 2021
Materias:	fullerene c60 rhodococci viability phenotypic heterogeneity. Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0e97e4275bb4dccb4d2a56cf45ab992
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spelling	oai:doaj.org-article:b0e97e4275bb4dccb4d2a56cf45ab9922021-12-02T17:40:15ZViability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C6010.38045/ohrm.2021.3.012887-34582587-3466https://doaj.org/article/b0e97e4275bb4dccb4d2a56cf45ab9922021-06-01T00:00:00Zhttps://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/83/91https://doaj.org/toc/2887-3458https://doaj.org/toc/2587-3466Introduction. In recent years, due to wide applications of nanotechnologies in various fields, the safety of nanomaterials has become a pressing issue. Fullerene C60 is not an exception. Research on the activity of microorganisms and their interaction with nanoparticles is of major importance, both for microorganisms and for the ecosystem as a whole. Material and methods. Fullerene C60powder was purchased from Sigma-Aldrich. The object of study was R. rhodochrous CNMN-Ac-05 strain. The number of viable bacterial cells was estimated by colony-forming units (CFU). The morphological features of the rhodococci colonies have been described according to the usual microbiological method. Results. It was established that fullerene C60 in concentrations of 1-25 mg/L fullerene C60 stimulated the growth of R. rhodochrous by 2.4-2.8 times. As the concentration of fullerene C60 increased up to 50-100 mg/L, the multiplication and growth of rhodococci decreased by 29.5% and 38% respectively. In the presence of 1-10 mg/L fullerene C60 the rhodococci population remained homogeneous, being composed of 100% S type colonies. The increase of fullerene C60 concentration led both to the decrease in the CFU number and to the appearance of R type colonies, up to 1.3% of population. Conclusions. Fullerene C60 in concentrations 1-100 mg/L had no obvious toxic effect on the rhodococci strain. The optimum concentration is 10 mg/L. The concentrations higher than 25 mg/L led to the dissociation of rhodococcal population and diminution in the CFU counts, but not to the total inhibition.Olga POSTOLACHIInna RASTIMESINAValentina JOSANAsociatia de Biosiguranta si Biosecuritatearticlefullerene c60rhodococciviabilityphenotypic heterogeneity.MedicineRScienceQENFRRORUOne Health & Risk Management, Vol 2, Iss 3, Pp 4-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR RO RU
topic	fullerene c60 rhodococci viability phenotypic heterogeneity. Medicine R Science Q
spellingShingle	fullerene c60 rhodococci viability phenotypic heterogeneity. Medicine R Science Q Olga POSTOLACHI Inna RASTIMESINA Valentina JOSAN Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60
description	Introduction. In recent years, due to wide applications of nanotechnologies in various fields, the safety of nanomaterials has become a pressing issue. Fullerene C60 is not an exception. Research on the activity of microorganisms and their interaction with nanoparticles is of major importance, both for microorganisms and for the ecosystem as a whole. Material and methods. Fullerene C60powder was purchased from Sigma-Aldrich. The object of study was R. rhodochrous CNMN-Ac-05 strain. The number of viable bacterial cells was estimated by colony-forming units (CFU). The morphological features of the rhodococci colonies have been described according to the usual microbiological method. Results. It was established that fullerene C60 in concentrations of 1-25 mg/L fullerene C60 stimulated the growth of R. rhodochrous by 2.4-2.8 times. As the concentration of fullerene C60 increased up to 50-100 mg/L, the multiplication and growth of rhodococci decreased by 29.5% and 38% respectively. In the presence of 1-10 mg/L fullerene C60 the rhodococci population remained homogeneous, being composed of 100% S type colonies. The increase of fullerene C60 concentration led both to the decrease in the CFU number and to the appearance of R type colonies, up to 1.3% of population. Conclusions. Fullerene C60 in concentrations 1-100 mg/L had no obvious toxic effect on the rhodococci strain. The optimum concentration is 10 mg/L. The concentrations higher than 25 mg/L led to the dissociation of rhodococcal population and diminution in the CFU counts, but not to the total inhibition.
format	article
author	Olga POSTOLACHI Inna RASTIMESINA Valentina JOSAN
author_facet	Olga POSTOLACHI Inna RASTIMESINA Valentina JOSAN
author_sort	Olga POSTOLACHI
title	Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60
title_short	Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60
title_full	Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60
title_fullStr	Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60
title_full_unstemmed	Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60
title_sort	viability and phenotypic heterogeneity of rhodococcus rhodochrous cnmn-ac-05 in the presence of fullerene c60
publisher	Asociatia de Biosiguranta si Biosecuritate
publishDate	2021
url	https://doaj.org/article/b0e97e4275bb4dccb4d2a56cf45ab992
work_keys_str_mv	AT olgapostolachi viabilityandphenotypicheterogeneityofrhodococcusrhodochrouscnmnac05inthepresenceoffullerenec60 AT innarastimesina viabilityandphenotypicheterogeneityofrhodococcusrhodochrouscnmnac05inthepresenceoffullerenec60 AT valentinajosan viabilityandphenotypicheterogeneityofrhodococcusrhodochrouscnmnac05inthepresenceoffullerenec60
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Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60

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