Translational control of enzyme scavenger expression with toxin-induced micro RNA switches

Abstract Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic netwo...

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Autores principales:	Nina M. Pollak, Justin J. Cooper-White, Joanne Macdonald
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0e97f0d97544fadb1b5f8b8a0c9ba75
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spelling	oai:doaj.org-article:b0e97f0d97544fadb1b5f8b8a0c9ba752021-12-02T14:16:26ZTranslational control of enzyme scavenger expression with toxin-induced micro RNA switches10.1038/s41598-021-81679-62045-2322https://doaj.org/article/b0e97f0d97544fadb1b5f8b8a0c9ba752021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81679-6https://doaj.org/toc/2045-2322Abstract Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxification functionality. We employed a modular design strategy by engineering toxin-induced control of an enzyme scavenger. Our miRNA switch results show moderate synthetic expression control over a biologically active detoxification enzyme molecule, using an established design protocol. However, following a new design approach, we demonstrated an evolutionarily designed miRNA switch to more effectively activate enzyme activity than synthetically designed versions, allowing markedly improved extrinsic user-defined control with a toxin as inducer. Our straightforward new design approach is simple to implement and uses easily accessible web-based databases and prediction tools. The ability to exert control of toxicity demonstrates potential for modular detoxification systems that provide a pathway to new therapeutic and biocomputing applications.Nina M. PollakJustin J. Cooper-WhiteJoanne MacdonaldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nina M. Pollak Justin J. Cooper-White Joanne Macdonald Translational control of enzyme scavenger expression with toxin-induced micro RNA switches
description	Abstract Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxification functionality. We employed a modular design strategy by engineering toxin-induced control of an enzyme scavenger. Our miRNA switch results show moderate synthetic expression control over a biologically active detoxification enzyme molecule, using an established design protocol. However, following a new design approach, we demonstrated an evolutionarily designed miRNA switch to more effectively activate enzyme activity than synthetically designed versions, allowing markedly improved extrinsic user-defined control with a toxin as inducer. Our straightforward new design approach is simple to implement and uses easily accessible web-based databases and prediction tools. The ability to exert control of toxicity demonstrates potential for modular detoxification systems that provide a pathway to new therapeutic and biocomputing applications.
format	article
author	Nina M. Pollak Justin J. Cooper-White Joanne Macdonald
author_facet	Nina M. Pollak Justin J. Cooper-White Joanne Macdonald
author_sort	Nina M. Pollak
title	Translational control of enzyme scavenger expression with toxin-induced micro RNA switches
title_short	Translational control of enzyme scavenger expression with toxin-induced micro RNA switches
title_full	Translational control of enzyme scavenger expression with toxin-induced micro RNA switches
title_fullStr	Translational control of enzyme scavenger expression with toxin-induced micro RNA switches
title_full_unstemmed	Translational control of enzyme scavenger expression with toxin-induced micro RNA switches
title_sort	translational control of enzyme scavenger expression with toxin-induced micro rna switches
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0e97f0d97544fadb1b5f8b8a0c9ba75
work_keys_str_mv	AT ninampollak translationalcontrolofenzymescavengerexpressionwithtoxininducedmicrornaswitches AT justinjcooperwhite translationalcontrolofenzymescavengerexpressionwithtoxininducedmicrornaswitches AT joannemacdonald translationalcontrolofenzymescavengerexpressionwithtoxininducedmicrornaswitches
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Translational control of enzyme scavenger expression with toxin-induced micro RNA switches

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