Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors

Matthieu F Dumont,1 Sridevi Yadavilli,2 Raymond W Sze,1,4 Javad Nazarian,2,3 Rohan Fernandes1,4,5 1Sheikh Zayed Institute for Pediatric Surgical Innovation, 2Center for Genetic Medicine Research, Children’s National Medical Center, Washington, DC, USA; 3Department of Integrative Systems...

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Autores principales:	Dumont MF, Yadavilli S, Sze RW, Nazarian J, Fernandes R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b0ea1e2712c94db482381fedf0191e49
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spelling	oai:doaj.org-article:b0ea1e2712c94db482381fedf0191e492021-12-02T01:35:23ZManganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors1178-2013https://doaj.org/article/b0ea1e2712c94db482381fedf0191e492014-05-01T00:00:00Zhttp://www.dovepress.com/manganese-containing-prussian-blue-nanoparticles-for-imaging-of-pediat-a16989https://doaj.org/toc/1178-2013 Matthieu F Dumont,1 Sridevi Yadavilli,2 Raymond W Sze,1,4 Javad Nazarian,2,3 Rohan Fernandes1,4,5 1Sheikh Zayed Institute for Pediatric Surgical Innovation, 2Center for Genetic Medicine Research, Children’s National Medical Center, Washington, DC, USA; 3Department of Integrative Systems Biology, 4Department of Radiology, 5Department of Pediatrics, George Washington University, Washington, DC, USA Abstract: Pediatric brain tumors (PBTs) are a leading cause of death in children. For an improved prognosis in patients with PBTs, there is a critical need to develop molecularly-specific imaging agents to monitor disease progression and response to treatment. In this paper, we describe manganese-containing Prussian blue nanoparticles as agents for molecular magnetic resonance imaging (MRI) and fluorescence-based imaging of PBTs. Our core-shell nanoparticles consist of a core lattice structure that incorporates and retains paramagnetic Mn2+ ions, and generates MRI contrast (both negative and positive). The biofunctionalized shell is comprised of fluorescent avidin, which serves the dual purpose of enabling fluorescence imaging and functioning as a platform for the attachment of biotinylated ligands that target PBTs. The surfaces of our nanoparticles are modified with biotinylated antibodies targeting neuron-glial antigen 2 or biotinylated transferrin. Both neuron-glial antigen 2 and the transferrin receptor are protein markers overexpressed in PBTs. We describe the synthesis, biofunctionalization, and characterization of these multimodal nanoparticles. Further, we demonstrate the MRI and fluorescence imaging capabilities of manganese-containing Prussian blue nanoparticles in vitro. Finally, we demonstrate the potential of these nanoparticles as PBT imaging agents by measuring their organ and brain biodistribution in an orthotopic mouse model of PBTs using ex vivo fluorescence imaging. Keywords: Prussian blue, nanoparticles, imaging, pediatric brain tumors, fluorescence, magnetic resonance imaging, manganese, multimodalDumont MFYadavilli SSze RWNazarian JFernandes RDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2581-2595 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Dumont MF Yadavilli S Sze RW Nazarian J Fernandes R Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors
description	Matthieu F Dumont,1 Sridevi Yadavilli,2 Raymond W Sze,1,4 Javad Nazarian,2,3 Rohan Fernandes1,4,5 1Sheikh Zayed Institute for Pediatric Surgical Innovation, 2Center for Genetic Medicine Research, Children’s National Medical Center, Washington, DC, USA; 3Department of Integrative Systems Biology, 4Department of Radiology, 5Department of Pediatrics, George Washington University, Washington, DC, USA Abstract: Pediatric brain tumors (PBTs) are a leading cause of death in children. For an improved prognosis in patients with PBTs, there is a critical need to develop molecularly-specific imaging agents to monitor disease progression and response to treatment. In this paper, we describe manganese-containing Prussian blue nanoparticles as agents for molecular magnetic resonance imaging (MRI) and fluorescence-based imaging of PBTs. Our core-shell nanoparticles consist of a core lattice structure that incorporates and retains paramagnetic Mn2+ ions, and generates MRI contrast (both negative and positive). The biofunctionalized shell is comprised of fluorescent avidin, which serves the dual purpose of enabling fluorescence imaging and functioning as a platform for the attachment of biotinylated ligands that target PBTs. The surfaces of our nanoparticles are modified with biotinylated antibodies targeting neuron-glial antigen 2 or biotinylated transferrin. Both neuron-glial antigen 2 and the transferrin receptor are protein markers overexpressed in PBTs. We describe the synthesis, biofunctionalization, and characterization of these multimodal nanoparticles. Further, we demonstrate the MRI and fluorescence imaging capabilities of manganese-containing Prussian blue nanoparticles in vitro. Finally, we demonstrate the potential of these nanoparticles as PBT imaging agents by measuring their organ and brain biodistribution in an orthotopic mouse model of PBTs using ex vivo fluorescence imaging. Keywords: Prussian blue, nanoparticles, imaging, pediatric brain tumors, fluorescence, magnetic resonance imaging, manganese, multimodal
format	article
author	Dumont MF Yadavilli S Sze RW Nazarian J Fernandes R
author_facet	Dumont MF Yadavilli S Sze RW Nazarian J Fernandes R
author_sort	Dumont MF
title	Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors
title_short	Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors
title_full	Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors
title_fullStr	Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors
title_full_unstemmed	Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors
title_sort	manganese-containing prussian blue nanoparticles for imaging of pediatric brain tumors
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/b0ea1e2712c94db482381fedf0191e49
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Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors

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