Arabidopsis antibody resources for functional studies in plants

Abstract Here we report creation of a unique and a very valuable resource for Plant Scientific community worldwide. In this era of post-genomics and modelling of multi-cellular systems using an integrative systems biology approach, better understanding of protein localization at sub-cellular, cellul...

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Autores principales:	Jaesung Oh, Michael Wilson, Kristine Hill, Nicola Leftley, Charlie Hodgman, Malcolm J. Bennett, Ranjan Swarup
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0edab4548f34b41bf5850959e515dd8
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spelling	oai:doaj.org-article:b0edab4548f34b41bf5850959e515dd82021-12-02T13:58:14ZArabidopsis antibody resources for functional studies in plants10.1038/s41598-020-78689-12045-2322https://doaj.org/article/b0edab4548f34b41bf5850959e515dd82020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78689-1https://doaj.org/toc/2045-2322Abstract Here we report creation of a unique and a very valuable resource for Plant Scientific community worldwide. In this era of post-genomics and modelling of multi-cellular systems using an integrative systems biology approach, better understanding of protein localization at sub-cellular, cellular and tissue levels is likely to result in better understanding of their function and role in cell and tissue dynamics, protein–protein interactions and protein regulatory networks. We have raised 94 antibodies against key Arabidopsis root proteins, using either small peptides or recombinant proteins. The success rate with the peptide antibodies was very low. We show that affinity purification of antibodies massively improved the detection rate. Of 70 protein antibodies, 38 (55%) antibodies could detect a signal with high confidence and 22 of these antibodies are of immunocytochemistry grade. The targets include key proteins involved in hormone synthesis, transport and perception, membrane trafficking related proteins and several sub cellular marker proteins. These antibodies are available from the Nottingham Arabidopsis Stock Centre.Jaesung OhMichael WilsonKristine HillNicola LeftleyCharlie HodgmanMalcolm J. BennettRanjan SwarupNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jaesung Oh Michael Wilson Kristine Hill Nicola Leftley Charlie Hodgman Malcolm J. Bennett Ranjan Swarup Arabidopsis antibody resources for functional studies in plants
description	Abstract Here we report creation of a unique and a very valuable resource for Plant Scientific community worldwide. In this era of post-genomics and modelling of multi-cellular systems using an integrative systems biology approach, better understanding of protein localization at sub-cellular, cellular and tissue levels is likely to result in better understanding of their function and role in cell and tissue dynamics, protein–protein interactions and protein regulatory networks. We have raised 94 antibodies against key Arabidopsis root proteins, using either small peptides or recombinant proteins. The success rate with the peptide antibodies was very low. We show that affinity purification of antibodies massively improved the detection rate. Of 70 protein antibodies, 38 (55%) antibodies could detect a signal with high confidence and 22 of these antibodies are of immunocytochemistry grade. The targets include key proteins involved in hormone synthesis, transport and perception, membrane trafficking related proteins and several sub cellular marker proteins. These antibodies are available from the Nottingham Arabidopsis Stock Centre.
format	article
author	Jaesung Oh Michael Wilson Kristine Hill Nicola Leftley Charlie Hodgman Malcolm J. Bennett Ranjan Swarup
author_facet	Jaesung Oh Michael Wilson Kristine Hill Nicola Leftley Charlie Hodgman Malcolm J. Bennett Ranjan Swarup
author_sort	Jaesung Oh
title	Arabidopsis antibody resources for functional studies in plants
title_short	Arabidopsis antibody resources for functional studies in plants
title_full	Arabidopsis antibody resources for functional studies in plants
title_fullStr	Arabidopsis antibody resources for functional studies in plants
title_full_unstemmed	Arabidopsis antibody resources for functional studies in plants
title_sort	arabidopsis antibody resources for functional studies in plants
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b0edab4548f34b41bf5850959e515dd8
work_keys_str_mv	AT jaesungoh arabidopsisantibodyresourcesforfunctionalstudiesinplants AT michaelwilson arabidopsisantibodyresourcesforfunctionalstudiesinplants AT kristinehill arabidopsisantibodyresourcesforfunctionalstudiesinplants AT nicolaleftley arabidopsisantibodyresourcesforfunctionalstudiesinplants AT charliehodgman arabidopsisantibodyresourcesforfunctionalstudiesinplants AT malcolmjbennett arabidopsisantibodyresourcesforfunctionalstudiesinplants AT ranjanswarup arabidopsisantibodyresourcesforfunctionalstudiesinplants
_version_	1718392221149954048

Arabidopsis antibody resources for functional studies in plants

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