Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients

Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients

Abstract Polycystic ovary syndrome (PCOS) is a common endocrine disease in females that is characterized by hyperandrogenemia, chronic anovulation, and polycystic ovaries. However, the exact etiology and pathogenesis of PCOS are still unknown. The aim of this study was to clarify the bacterial, stre...

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Autores principales: Fu Chen, Zhangran Chen, Minjie Chen, Guishan Chen, Qingxia Huang, Xiaoping Yang, Huihuang Yin, Lan Chen, Weichun Zhang, Hong Lin, Miaoqiong Ou, Luanhong Wang, Yongsong Chen, Chujia Lin, Wencan Xu, Guoshu Yin
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Microbial ecology
QR100-130
Acceso en línea:https://doaj.org/article/b0ee7f2f94374437be9ac9a4eb8619ec
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spelling oai:doaj.org-article:b0ee7f2f94374437be9ac9a4eb8619ec2021-12-02T15:33:14ZReduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients10.1038/s41522-021-00231-62055-5008https://doaj.org/article/b0ee7f2f94374437be9ac9a4eb8619ec2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00231-6https://doaj.org/toc/2055-5008Abstract Polycystic ovary syndrome (PCOS) is a common endocrine disease in females that is characterized by hyperandrogenemia, chronic anovulation, and polycystic ovaries. However, the exact etiology and pathogenesis of PCOS are still unknown. The aim of this study was to clarify the bacterial, stress status, and metabolic differences in the gut microbiomes of healthy individuals and patients with high body mass index (BMI) PCOS (PCOS-HB) and normal BMI PCOS (PCOS-LB), respectively. Here, we compared the gut microbiota characteristics of PCOS-HB, PCOS-LB, and healthy controls by 16S rRNA gene sequencing, FK506-binding protein 5 (FKBP5) DNA methylation and plasma metabolite determination. Clinical parameter comparisons indicated that PCOS patients had higher concentrations of total testosterone, androstenedione, dehydroepiandrosterone sulfate, luteinizing hormone, and HOMA-IR while lower FKBP5 DNA methylation. Significant differences in bacterial diversity and community were observed between the PCOS and healthy groups but not between the PCOS-HB and PCOS-LB groups. Bacterial species number was negatively correlated with insulin concentrations (both under fasting status and 120 min after glucose load) and HOMA-IR but positively related to FKBP5 DNA methylation. Compared to the healthy group, both PCOS groups had significant changes in bacterial genera, including Prevotella_9, Dorea, Maihella, and Slackia, and plasma metabolites, including estrone sulfate, lysophosphatidyl choline 18:2, and phosphatidylcholine (22:6e/19:1). The correlation network revealed the complicated interaction of the clinical index, bacterial genus, stress indices, and metabolites. Our work links the stress responses and gut microbiota characteristics of PCOS disease, which might afford perspectives to understand the progression of PCOS.Fu ChenZhangran ChenMinjie ChenGuishan ChenQingxia HuangXiaoping YangHuihuang YinLan ChenWeichun ZhangHong LinMiaoqiong OuLuanhong WangYongsong ChenChujia LinWencan XuGuoshu YinNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Microbial ecology
QR100-130
spellingShingle Microbial ecology
QR100-130
Fu Chen
Zhangran Chen
Minjie Chen
Guishan Chen
Qingxia Huang
Xiaoping Yang
Huihuang Yin
Lan Chen
Weichun Zhang
Hong Lin
Miaoqiong Ou
Luanhong Wang
Yongsong Chen
Chujia Lin
Wencan Xu
Guoshu Yin
Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients
description Abstract Polycystic ovary syndrome (PCOS) is a common endocrine disease in females that is characterized by hyperandrogenemia, chronic anovulation, and polycystic ovaries. However, the exact etiology and pathogenesis of PCOS are still unknown. The aim of this study was to clarify the bacterial, stress status, and metabolic differences in the gut microbiomes of healthy individuals and patients with high body mass index (BMI) PCOS (PCOS-HB) and normal BMI PCOS (PCOS-LB), respectively. Here, we compared the gut microbiota characteristics of PCOS-HB, PCOS-LB, and healthy controls by 16S rRNA gene sequencing, FK506-binding protein 5 (FKBP5) DNA methylation and plasma metabolite determination. Clinical parameter comparisons indicated that PCOS patients had higher concentrations of total testosterone, androstenedione, dehydroepiandrosterone sulfate, luteinizing hormone, and HOMA-IR while lower FKBP5 DNA methylation. Significant differences in bacterial diversity and community were observed between the PCOS and healthy groups but not between the PCOS-HB and PCOS-LB groups. Bacterial species number was negatively correlated with insulin concentrations (both under fasting status and 120 min after glucose load) and HOMA-IR but positively related to FKBP5 DNA methylation. Compared to the healthy group, both PCOS groups had significant changes in bacterial genera, including Prevotella_9, Dorea, Maihella, and Slackia, and plasma metabolites, including estrone sulfate, lysophosphatidyl choline 18:2, and phosphatidylcholine (22:6e/19:1). The correlation network revealed the complicated interaction of the clinical index, bacterial genus, stress indices, and metabolites. Our work links the stress responses and gut microbiota characteristics of PCOS disease, which might afford perspectives to understand the progression of PCOS.
format article
author Fu Chen
Zhangran Chen
Minjie Chen
Guishan Chen
Qingxia Huang
Xiaoping Yang
Huihuang Yin
Lan Chen
Weichun Zhang
Hong Lin
Miaoqiong Ou
Luanhong Wang
Yongsong Chen
Chujia Lin
Wencan Xu
Guoshu Yin
author_facet Fu Chen
Zhangran Chen
Minjie Chen
Guishan Chen
Qingxia Huang
Xiaoping Yang
Huihuang Yin
Lan Chen
Weichun Zhang
Hong Lin
Miaoqiong Ou
Luanhong Wang
Yongsong Chen
Chujia Lin
Wencan Xu
Guoshu Yin
author_sort Fu Chen
title Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients
title_short Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients
title_full Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients
title_fullStr Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients
title_full_unstemmed Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients
title_sort reduced stress-associated fkbp5 dna methylation together with gut microbiota dysbiosis is linked with the progression of obese pcos patients
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/b0ee7f2f94374437be9ac9a4eb8619ec
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