The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.

Purpose: The efficiency of callus induction as source of bioactive compound is a critical step in the cell suspension culture for commercial production of important secondary metabolites. One of the main factors affecting callus efficiency is the developmental stage of explants. On the other hands,...

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Autores principales:	Seyed Mahdi Ziaratnia, Somayeh Amini
Formato:	article
Lenguaje:	EN
Publicado:	University of Birjand 2021
Materias:	callogenesis culture medium developmental stage plant growth regulators saffron Agriculture S
Acceso en línea:	https://doaj.org/article/b0f4aee9c7cd4bf9a6aadf1cabbb4cec
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spelling	oai:doaj.org-article:b0f4aee9c7cd4bf9a6aadf1cabbb4cec2021-11-06T04:22:47ZThe effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.2588-48832588-616910.22077/jhpr.2021.3684.1166https://doaj.org/article/b0f4aee9c7cd4bf9a6aadf1cabbb4cec2021-08-01T00:00:00Zhttps://jhpr.birjand.ac.ir/article_1700_5be02522351884bfe9945ad5c00bfc75.pdfhttps://doaj.org/toc/2588-4883https://doaj.org/toc/2588-6169Purpose: The efficiency of callus induction as source of bioactive compound is a critical step in the cell suspension culture for commercial production of important secondary metabolites. One of the main factors affecting callus efficiency is the developmental stage of explants. On the other hands, it has been declared that the optimization of medium composition has a significant influence on callogenesis and increment of biomass. Research method: The aim of this study was the evaluation of different developmental stages of saffron corms as a source of explant preparation (immature and mature corms), plant growth regulators (PGRs) combinations (1-Naphthaleneacetic acid (NAA); 2, 4-dichlorophenoxyacetic acid (2,4-D); 6-Benzyladenine (BA) and kinetin (Kin)) and type of medium (Gamborg (B5) medium and Murashige and Skoog (MS) medium) in callus induction in saffron corms to increase cell biomass. The media were supplemented with different combinations of 1-Naphthaleneacetic acid or 2, 4-dichlorophenoxyacetic acid (2, 4, 8 mgL-1) as auxins and 6-Benzyladenine or kinetin (1, 4, 8 mgL-1) as cytokinin. Findings: The results showed that mature corms harvested in May had the best developmental stage for callogenesis. The maximum callus formation was recorded in B5 medium supplemented with 2,4-dichlorophenoxyacetic acid (4 mgL-1) and kinetin (1 mgL-1) with 2.61 g fresh weight. Limitations: No limitations to report. Originality/Value: This protocol for sampling explant and callus formation was found to make suitable sources of plant material for further study in production of bioactive compounds via cell suspension cultures.Seyed Mahdi ZiaratniaSomayeh AminiUniversity of Birjandarticlecallogenesisculture mediumdevelopmental stageplant growth regulatorssaffronAgricultureSENJournal of Horticulture and Postharvest Research, Vol 4, Iss Special Issue - Recent Advances in Saffron, Pp 43-56 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	callogenesis culture medium developmental stage plant growth regulators saffron Agriculture S
spellingShingle	callogenesis culture medium developmental stage plant growth regulators saffron Agriculture S Seyed Mahdi Ziaratnia Somayeh Amini The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.
description	Purpose: The efficiency of callus induction as source of bioactive compound is a critical step in the cell suspension culture for commercial production of important secondary metabolites. One of the main factors affecting callus efficiency is the developmental stage of explants. On the other hands, it has been declared that the optimization of medium composition has a significant influence on callogenesis and increment of biomass. Research method: The aim of this study was the evaluation of different developmental stages of saffron corms as a source of explant preparation (immature and mature corms), plant growth regulators (PGRs) combinations (1-Naphthaleneacetic acid (NAA); 2, 4-dichlorophenoxyacetic acid (2,4-D); 6-Benzyladenine (BA) and kinetin (Kin)) and type of medium (Gamborg (B5) medium and Murashige and Skoog (MS) medium) in callus induction in saffron corms to increase cell biomass. The media were supplemented with different combinations of 1-Naphthaleneacetic acid or 2, 4-dichlorophenoxyacetic acid (2, 4, 8 mgL-1) as auxins and 6-Benzyladenine or kinetin (1, 4, 8 mgL-1) as cytokinin. Findings: The results showed that mature corms harvested in May had the best developmental stage for callogenesis. The maximum callus formation was recorded in B5 medium supplemented with 2,4-dichlorophenoxyacetic acid (4 mgL-1) and kinetin (1 mgL-1) with 2.61 g fresh weight. Limitations: No limitations to report. Originality/Value: This protocol for sampling explant and callus formation was found to make suitable sources of plant material for further study in production of bioactive compounds via cell suspension cultures.
format	article
author	Seyed Mahdi Ziaratnia Somayeh Amini
author_facet	Seyed Mahdi Ziaratnia Somayeh Amini
author_sort	Seyed Mahdi Ziaratnia
title	The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.
title_short	The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.
title_full	The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.
title_fullStr	The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.
title_full_unstemmed	The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.
title_sort	effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of crocus sativus l.
publisher	University of Birjand
publishDate	2021
url	https://doaj.org/article/b0f4aee9c7cd4bf9a6aadf1cabbb4cec
work_keys_str_mv	AT seyedmahdiziaratnia theeffectofdevelopmentalstagesofcormtypeofmediumandplantgrowthregulatorsincallusinductionofcrocussativusl AT somayehamini theeffectofdevelopmentalstagesofcormtypeofmediumandplantgrowthregulatorsincallusinductionofcrocussativusl AT seyedmahdiziaratnia effectofdevelopmentalstagesofcormtypeofmediumandplantgrowthregulatorsincallusinductionofcrocussativusl AT somayehamini effectofdevelopmentalstagesofcormtypeofmediumandplantgrowthregulatorsincallusinductionofcrocussativusl
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The effect of developmental stages of corm, type of medium and plant growth regulators in callus induction of Crocus sativus L.

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