Chlorogenic acid inhibits human platelet activation and thrombus formation.

<h4>Background</h4>Chlorogenic acid is a potent phenolic antioxidant. However, its effect on platelet aggregation, a critical factor in arterial thrombosis, remains unclear. Consequently, chlorogenic acid-action mechanisms in preventing platelet activation and thrombus formation were exa...

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Autores principales:	Eduardo Fuentes, Julio Caballero, Marcelo Alarcón, Armando Rojas, Iván Palomo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0f9614acde64868889b9b3078452c50
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spelling	oai:doaj.org-article:b0f9614acde64868889b9b3078452c502021-11-18T08:29:43ZChlorogenic acid inhibits human platelet activation and thrombus formation.1932-620310.1371/journal.pone.0090699https://doaj.org/article/b0f9614acde64868889b9b3078452c502014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24598787/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Chlorogenic acid is a potent phenolic antioxidant. However, its effect on platelet aggregation, a critical factor in arterial thrombosis, remains unclear. Consequently, chlorogenic acid-action mechanisms in preventing platelet activation and thrombus formation were examined.<h4>Methods and results</h4>Chlorogenic acid in a dose-dependent manner (0.1 to 1 mmol/L) inhibited platelet secretion and aggregation induced by ADP, collagen, arachidonic acid and TRAP-6, and diminished platelet firm adhesion/aggregation and platelet-leukocyte interactions under flow conditions. At these concentrations chlorogenic acid significantly decreased platelet inflammatory mediators (sP-selectin, sCD40L, CCL5 and IL-1β) and increased intraplatelet cAMP levels/PKA activation. Interestingly, SQ22536 (an adenylate cyclase inhibitor) and ZM241385 (a potent A2A receptor antagonist) attenuated the antiplatelet effect of chlorogenic acid. Chlorogenic acid is compatible to the active site of the adenosine A2A receptor as revealed through molecular modeling. In addition, chlorogenic acid had a significantly lower effect on mouse bleeding time when compared to the same dose of aspirin.<h4>Conclusions</h4>Antiplatelet and antithrombotic effects of chlorogenic acid are associated with the A2A receptor/adenylate cyclase/cAMP/PKA signaling pathway.Eduardo FuentesJulio CaballeroMarcelo AlarcónArmando RojasIván PalomoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e90699 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eduardo Fuentes Julio Caballero Marcelo Alarcón Armando Rojas Iván Palomo Chlorogenic acid inhibits human platelet activation and thrombus formation.
description	<h4>Background</h4>Chlorogenic acid is a potent phenolic antioxidant. However, its effect on platelet aggregation, a critical factor in arterial thrombosis, remains unclear. Consequently, chlorogenic acid-action mechanisms in preventing platelet activation and thrombus formation were examined.<h4>Methods and results</h4>Chlorogenic acid in a dose-dependent manner (0.1 to 1 mmol/L) inhibited platelet secretion and aggregation induced by ADP, collagen, arachidonic acid and TRAP-6, and diminished platelet firm adhesion/aggregation and platelet-leukocyte interactions under flow conditions. At these concentrations chlorogenic acid significantly decreased platelet inflammatory mediators (sP-selectin, sCD40L, CCL5 and IL-1β) and increased intraplatelet cAMP levels/PKA activation. Interestingly, SQ22536 (an adenylate cyclase inhibitor) and ZM241385 (a potent A2A receptor antagonist) attenuated the antiplatelet effect of chlorogenic acid. Chlorogenic acid is compatible to the active site of the adenosine A2A receptor as revealed through molecular modeling. In addition, chlorogenic acid had a significantly lower effect on mouse bleeding time when compared to the same dose of aspirin.<h4>Conclusions</h4>Antiplatelet and antithrombotic effects of chlorogenic acid are associated with the A2A receptor/adenylate cyclase/cAMP/PKA signaling pathway.
format	article
author	Eduardo Fuentes Julio Caballero Marcelo Alarcón Armando Rojas Iván Palomo
author_facet	Eduardo Fuentes Julio Caballero Marcelo Alarcón Armando Rojas Iván Palomo
author_sort	Eduardo Fuentes
title	Chlorogenic acid inhibits human platelet activation and thrombus formation.
title_short	Chlorogenic acid inhibits human platelet activation and thrombus formation.
title_full	Chlorogenic acid inhibits human platelet activation and thrombus formation.
title_fullStr	Chlorogenic acid inhibits human platelet activation and thrombus formation.
title_full_unstemmed	Chlorogenic acid inhibits human platelet activation and thrombus formation.
title_sort	chlorogenic acid inhibits human platelet activation and thrombus formation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b0f9614acde64868889b9b3078452c50
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Chlorogenic acid inhibits human platelet activation and thrombus formation.

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