Mechanism of autonomous synchronization of the circadian KaiABC rhythm

Abstract The cyanobacterial circadian clock can be reconstituted by mixing three proteins, KaiA, KaiB, and KaiC, in vitro. In this protein mixture, oscillations of the phosphorylation level of KaiC molecules are synchronized to show the coherent oscillations of the ensemble of many molecules. Howeve...

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Autor principal:	Masaki Sasai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0fc52bbd2fb4a19a4e9aa1b31843ff1
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spelling	oai:doaj.org-article:b0fc52bbd2fb4a19a4e9aa1b31843ff12021-12-02T15:53:43ZMechanism of autonomous synchronization of the circadian KaiABC rhythm10.1038/s41598-021-84008-z2045-2322https://doaj.org/article/b0fc52bbd2fb4a19a4e9aa1b31843ff12021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84008-zhttps://doaj.org/toc/2045-2322Abstract The cyanobacterial circadian clock can be reconstituted by mixing three proteins, KaiA, KaiB, and KaiC, in vitro. In this protein mixture, oscillations of the phosphorylation level of KaiC molecules are synchronized to show the coherent oscillations of the ensemble of many molecules. However, the molecular mechanism of this synchronization has not yet been fully elucidated. In this paper, we explain a theoretical model that considers the multifold feedback relations among the structure and reactions of KaiC. The simulated KaiC hexamers show stochastic switch-like transitions at the level of single molecules, which are synchronized in the ensemble through the sequestration of KaiA into the KaiC–KaiB–KaiA complexes. The proposed mechanism quantitatively reproduces the synchronization that was observed by mixing two solutions oscillating in different phases. The model results suggest that biochemical assays with varying concentrations of KaiA or KaiB can be used to test this hypothesis.Masaki SasaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masaki Sasai Mechanism of autonomous synchronization of the circadian KaiABC rhythm
description	Abstract The cyanobacterial circadian clock can be reconstituted by mixing three proteins, KaiA, KaiB, and KaiC, in vitro. In this protein mixture, oscillations of the phosphorylation level of KaiC molecules are synchronized to show the coherent oscillations of the ensemble of many molecules. However, the molecular mechanism of this synchronization has not yet been fully elucidated. In this paper, we explain a theoretical model that considers the multifold feedback relations among the structure and reactions of KaiC. The simulated KaiC hexamers show stochastic switch-like transitions at the level of single molecules, which are synchronized in the ensemble through the sequestration of KaiA into the KaiC–KaiB–KaiA complexes. The proposed mechanism quantitatively reproduces the synchronization that was observed by mixing two solutions oscillating in different phases. The model results suggest that biochemical assays with varying concentrations of KaiA or KaiB can be used to test this hypothesis.
format	article
author	Masaki Sasai
author_facet	Masaki Sasai
author_sort	Masaki Sasai
title	Mechanism of autonomous synchronization of the circadian KaiABC rhythm
title_short	Mechanism of autonomous synchronization of the circadian KaiABC rhythm
title_full	Mechanism of autonomous synchronization of the circadian KaiABC rhythm
title_fullStr	Mechanism of autonomous synchronization of the circadian KaiABC rhythm
title_full_unstemmed	Mechanism of autonomous synchronization of the circadian KaiABC rhythm
title_sort	mechanism of autonomous synchronization of the circadian kaiabc rhythm
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b0fc52bbd2fb4a19a4e9aa1b31843ff1
work_keys_str_mv	AT masakisasai mechanismofautonomoussynchronizationofthecircadiankaiabcrhythm
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Mechanism of autonomous synchronization of the circadian KaiABC rhythm

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